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The volatile organic compounds of Floccularia luteovirens modulate plant growth and metabolism in Arabidopsis thaliana

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Abstract

Aims

The volatile organic compounds (VOCs) produced by soil microbes modulated plant growth and development. Floccularia luteovirens, an edible mushroom, is beneficial to the growth of alpine meadow plants on the Qinghai-Tibet Plateau. We aimed to elucidate the physiological and molecular mechanisms underlying the mushroom fungal VOC-mediated plant growth and development.

Methods

Here, we investigated the effects of VOCs produced by F. luteovirens on the root system development and seedling growth by integrating physiology, genetics, transcriptome and metabolome analysis using 1/2 MS medium-grown Arabidopsis thaliana seedlings.

Results

Treatment with F. luteovirens VOCs reduce primary root growth by aggravating auxin accumulation through the repression of the abundance of auxin efflux carrier PIN-FORMED 2 (PIN2) protein, whereas it increases the lateral root number of A. thaliana seedlings. In addition to modulating root system architecture, treatment with F. luteovirens VOCs markedly increased aboveground growth. The transcriptome and metabolome analyses further supported the notion that F. luteovirens VOCs modulate plant growth and development through the induction of carbon/nitrogen metabolism and antioxidant defense while repressing several secondary metabolism and amino acid catabolism pathways.

Conclusions

These results suggested that application of F. luteovirens VOCs promote growth by inducing changes in root system architecture through auxin pathway and regulating metabolism in plants.

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Acknowledgments

This research was supported by the National Key Research and Development Program of China (2016YFC0501901), the Basic Research Program of Qinghai Province (2019-ZJ-7033), Qinghai innovation platform construction project (2017-ZJ-Y20), the China National Natural Sciences Foundation (31772383, 31960268, 41761107), the Second Tibetan Plateau Scientific Expedition and Research (STEP) Program (2019QZKK0302) and Key Special Foundation/Project of Science and Technology Department of Qinghai Province (2019-SF-A12).

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Authors and Affiliations

  1. College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China

    Liangliang Sun & Jin Xu

  2. College of Geosciences, Qinghai Normal University, Xining, 810008, China

    Ming Cao & Wenying Wang

  3. College of Life Science, Qinghai Normal University, Xining, 810008, China

    Fei Liu & Wenying Wang

  4. GanSu Key Laboratory for Utilization of Agricultural Solid Waste Resources, College of Bioengineering and Biotechnology, TianShui Normal University, TianShui, 741000, GanSu, China

    Yibo Wang

  5. CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, 666303, Yunnan, China

    Jinpeng Wan & Ruling Wang

  6. Key Laboratory of Restoration Ecology of Cold Area in Qinghai Province, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, China

    Huakun Zhou

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  1. Liangliang Sun
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  2. Ming Cao
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  3. Fei Liu
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  4. Yibo Wang
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  5. Jinpeng Wan
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  6. Ruling Wang
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  7. Huakun Zhou
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  8. Wenying Wang
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  9. Jin Xu
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Correspondence to Huakun Zhou, Wenying Wang or Jin Xu.

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Sun, L., Cao, M., Liu, F. et al. The volatile organic compounds of Floccularia luteovirens modulate plant growth and metabolism in Arabidopsis thaliana. Plant Soil 456, 207–221 (2020). https://doi.org/10.1007/s11104-020-04709-8

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