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Characterization of depth-related bacterial communities and their relationships with the environmental factors in the river sediments

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Abstract

To better understand the bacterial processes in river sediments, it is necessary to investigate the depth-related bacterial communities in the whole sediment profile. Sediment samples were collected to a depth of 25 cm from the Pearl River. Bacterial abundance, activity, cell-specific respiration rate, and diversity were measured, respectively, by 4′, 6-diamidino-2-phenylindole direct count, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) staining, electron transport system by CTC reduction, and denaturing gradient gel electrophoresis analysis of 16S rRNA amplification fragments. Results showed that the bacterial metabolism activities decreased with the sediment depth. The total bacterial abundance was highest in the surface sediment with 65.1 × 107 cells g−1, and decreased to 11.1 × 107 cells g−1 below 20 cm in the sample location that suffered from heavy sewage inputs. The active bacteria accounted for 7.50–46.7% of the total bacterial number and decreased with the sediment depth. Electron transport system by the CTC reduction showed that bacterial respiration rate declined from 1.093 μmol CTC-formazan h−1 g−1 in the surface sediment to a half in the bottom sediment, while the cell-specific respiration increased significantly with the depth from 3.56 to 93.75 fmol CTC-formazan cell−1. The bacterial diversity also changed with the depth. Beta-Proteobacteria were the dominant species in the surface sediment, whereas Delta-Proteobacteria were the main species below 10 cm. Results of canonical correspondence analysis (CCA) indicated that the distribution of bacteria was affected by the combined effect of various dissolved inorganic matter, while the respiration rate was independent of the nutrient conditions. The specific bacterial distribution contributed to not only the nutrient cycle but also enhanced pollutant decomposition in sediment of the Pearl River. The results showed that some specific bacterial species had a strong activity in the deeper layers. Therefore, the metabolic functions of the deeper bacterial species should not be neglected.

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Acknowledgments

This work was partly supported by grants from the Chinese National Natural Science Foundation (Nos. 51039007 and 50779080) and the Yat-sen Innovative Talents Cultivation Program for Excellent Tutors.

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, Guangdong, 510275, China

    Shan Huang, Chen Chen, Yanyang Wu, Qunhe Wu & Renduo Zhang

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  1. Shan Huang
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  2. Chen Chen
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  3. Yanyang Wu
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  4. Qunhe Wu
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Correspondence to Qunhe Wu.

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Huang, S., Chen, C., Wu, Y. et al. Characterization of depth-related bacterial communities and their relationships with the environmental factors in the river sediments. World J Microbiol Biotechnol 27, 2655–2664 (2011). https://doi.org/10.1007/s11274-011-0739-x

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  • DOI: https://doi.org/10.1007/s11274-011-0739-x

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