Abstract
Introduction
An aerobic denitrifier was isolated from the Hua-Jia-Chi pond in China and identified as Pseudomonas mendocina 3-7 (Genbank No. HQ285879). This isolated strain could express periplasmic nitrate reductase which is essential for aerobic denitrification occurred when the dissolved oxygen (DO) level maintains at 3–10 mg L−1.
Methods
To determine whether the ability of isolated strain is exhibited in the bioremediation of polluted drinking source water, the heterotrophic nitrification and aerobic denitrification characteristics of P. mendocina 3-7 under different cultural conditions such as oxygen level, nitrate and organic concentrations were studied from the nitrogenous balance in the paper.
Results and conclusions
By measuring the nitrogen balance in all experiments under different culture conditions, the removal of total organic carbon and ammonium was positively correlated with total nitrogen removal, especially under high substrate level. With substrate concentration decreasing, ammonium and nitrate removal occurred separately, and ammonium was completely utilized first under low substrate concentration. Compared to that under high substrate level, the specific growth rate of P. mendocina 3-7 was not low under the low substrate level and the pollutant removal efficiencies remained high, which implies the stronger nitrogen removal and acclimatization capacities of the strain in oligotrophic niches.
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Acknowledgments
The work is funded by the National Key Technologies Research and Development Program of China (No. 2006BAJ08B01/2012BAJ25B07), the National Natural Science Foundation of China (No. 51078327/51008269), and the Fundamental Research Funds for the Central Universities.
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Zhu, L., Ding, W., Feng, Lj. et al. Characteristics of an aerobic denitrifier that utilizes ammonium and nitrate simultaneously under the oligotrophic niche. Environ Sci Pollut Res 19, 3185–3191 (2012). https://doi.org/10.1007/s11356-012-0822-3
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DOI: https://doi.org/10.1007/s11356-012-0822-3