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Erschienen in:

2015 | Online First | Buchkapitel

26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells

verfasst von : Malin Hernebring

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

The proteasome is a complex multisubunit protease that plays a major role in the degradation of proteins in eukaryotic cells. Proteasome function is one of the key players regulating the proteome and it is vital for many cellular processes. The method described here makes it possible to assay the proteolytic capacities of proteasome complexes separately in crude cytosolic extracts from ES cells. The method is based on hydrolysis of a fluorogenic peptide substrate in lysates prepared under conditions that favor the interactions of the 20S proteasomal catalytical core with either the 19S or the PA28αβ proteasome regulator.
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Metadaten
Titel
26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells
verfasst von
Malin Hernebring
Copyright-Jahr
2015
Verlag
Springer New York
DOI
https://doi.org/10.1007/7651_2015_216