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Erschienen in:

2015 | Online First | Buchkapitel

A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells

verfasst von : Peter F. Renz, Tobias A. Beyer

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

Human embryonic stem cells hold great promise for future biomedical applications such as disease modeling and regenerative medicine. However, these cells are notoriously difficult to culture and are refractory to common means of genetic manipulation, thereby limiting their range of applications. In this protocol, we present an easy and robust method of gene repression in human embryonic stem cells using lipofection of small interfering RNA (siRNA).
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Metadaten
Titel
A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells
verfasst von
Peter F. Renz
Tobias A. Beyer
Copyright-Jahr
2015
Verlag
Springer New York
DOI
https://doi.org/10.1007/7651_2015_212