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2019 | OriginalPaper | Buchkapitel

A Multimodular System to Study the Impact of a Focal Lesion in Neuronal Cell Cultures

verfasst von : Alberto Averna, Marta Carè, Stefano Buccelli, Marianna Semprini, Francesco Difato, Michela Chiappalone

Erschienen in: Hybrid Systems Biology

Verlag: Springer International Publishing

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Abstract

Characterizing neuronal networks activity and their dynamical changes due to endogenous and exogenous causes is a key issue of computational neuroscience and constitutes a fundamental contribution towards the development of innovative intervention strategies in case of brain damage. We address this challenge by making use of a multimodular system able to confine the growth of cells on substrate-embedded microelectrode arrays to investigate the interactions between networks of neurons. We observed their spontaneous and electrically induced network activity before and after a laser cut disconnecting one of the modules from all the others. We found that laser dissection induced de-synchronized activity among different modules during spontaneous activity, and prevented the propagation of evoked responses among modules during electrical stimulation. This reproducible experimental model constitutes a test-bed for the design and development of innovative computational tools for characterizing neural damage, and of novel neuro-prostheses aimed at restoring lost neuronal functionality between distinct brain areas.

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Nächstes Kapitel Reachability Analysis and Hybrid Systems Biology - In Memoriam Oded Maler

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Titel: A Multimodular System to Study the Impact of a Focal Lesion in Neuronal Cell Cultures
verfasst von: Alberto Averna
Marta Carè
Stefano Buccelli
Marianna Semprini
Francesco Difato
Michela Chiappalone
Verlag: Springer International Publishing
Buch: Hybrid Systems Biology
Print ISBN: 978-3-030-28041-3

Electronic ISBN: 978-3-030-28042-0

Copyright-Jahr: 2019
DOI: https://doi.org/10.1007/978-3-030-28042-0_1

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