nach oben

Erschienen in:

2012 | OriginalPaper | Buchkapitel

Action Potentials in Heart Cells

verfasst von : Lars Kaestner, Qinghai Tian, Peter Lipp

Erschienen in: Fluorescent Proteins II

Verlag: Springer Berlin Heidelberg

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

Action potentials are a basic and fast communication mode within or in between neuronal and muscular cells in the human body. The rhythmic initiation and structured propagation of action potentials in the heart are most essential for a vital organism. The use of genetically encoded biosensors based on fluorescent proteins allows a non-invasive biocompatible way to read-out action potentials in cardiac myocytes. This comprises the physiological situation as well as pathophysiological models of the diseased heart. Although the approaches to design such biosensors date back to the time when the first fluorescent protein-based FRET sensors were constructed, it took 15 years until first reliable sensors became available. In this chapter, it is shown in cardiac myocytes how fluorescent protein-based action potential measurements can be used in pharmacological screening applications as well as in basic biomedical research. Potentials and limitations will be discussed and perspectives of possible future developments will be provided.

Graphical Abstract

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Vorheriges Kapitel Fluorescent Genetically Encoded Calcium Indicators and Their In Vivo Application

Nächstes Kapitel Probing Structure and Dynamics of the Cell Membrane with Single Fluorescent Proteins

Viero C, Kraushaar U, Ruppenthal S, Kaestner L, Lipp P (2008) A primary culture system for sustained expression of a calcium sensor in preserved adult rat ventricular myocytes. Cell Calcium 43:59–71CrossRef

(1991) The Sicilian gambit. A new approach to the classification of antiarrhythmic drugs based on their actions on arrhythmogenic mechanisms. Task Force of the working group on Arrhythmias of the European Society of Cardiology. Circulation 84:1831–1851

Matteucci C (1842) Sur un phenomene physiologique produit par les muscles en contraction. Ann Chim Phys 6:339–341

Bois-Reymond (ed) (1848) Untersuchungen über Thierische Elektricität. Verlag von G. Reimer, Berlin

Hv H (1867) Handbuch der physiologischen Optik. Leopold Voss, Leipzig

Cole KS (1979) Mostly membranes (Kenneth S. Cole). Annu Rev Physiol 41:1–24CrossRef

Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391:85–100CrossRef

Sigworth FJ (1986) The patch clamp is more useful than anyone had expected. Fed Proc 45:2673–2677

Kaestner L, Lipp P (2007) Towards imaging the dynamics of protein signalling. In: Shorte SL, Frischknecht F (eds) Imaging cellular and molecular biological functions. Springer, Berlin, pp 289–312CrossRef

10.

ICH. (2005) S7B nonclinical evaluation of the potential for delayed ventricular repolarization (QT interval prolongation) by human pharmaceuticals. FDA & EMEA, pp 1–10

11.

Arrigoni C, Crivori P (2007) Assessment of QT liabilities in drug development. Cell Biol Toxicol 23:1–13CrossRef

12.

Lipp P, Kaestner L (2006) Image based high content screening – a view from basic science. In: Hüser J (ed) High-throughput screening in drug discovery. Wiley VCH, Weinheim, pp 129–149CrossRef

13.

Kaestner L, Scholz A, Hammer K, Vecerdea A, Ruppenthal S, Lipp P (2009) Isolation and genetic manipulation of adult cardiac myocytes for confocal imaging. J Vis Exp 31

14.

Hammer K, Ruppenthal S, Viero C, Scholz A, Edelmann L, Kaestner L, Lipp P (2010) Remodelling of Ca(2+) handling organelles in adult rat ventricular myocytes during long term culture. J Mol Cell Cardiol 49:427–437CrossRef

15.

Hardy ME, Pollard CE, Small BG, Bridgland-Taylor M, Woods AJ, Valentin JP, Abi-Gerges N (2009) Validation of a voltage-sensitive dye (di-4-ANEPPS)-based method for assessing drug-induced delayed repolarisation in beagle dog left ventricular midmyocardial myocytes. J Pharmacol Toxicol Meth 60:94–106CrossRef

16.

Bullen A, Saggau P (1999) High-speed, random-access fluorescence microscopy: II fast quantitative measurements with voltage-sensitive dyes. Biophys J 76:2272–2287CrossRef

17.

Bu G, Adams H, Berbari EJ, Rubart M (2009) Uniform action potential repolarization within the sarcolemma of in situ ventricular cardiomyocytes. Biophys J 96:2532–2546CrossRef

18.

Coates C, Fowler B, Holst G (2009) Scientific CMOS technology - a high-performance imaging breakthrough. In: FI Andor Technology PLC, PCO AG (ed) www.scmos.com. Andor Technology PLC, Fairchild imaging, PCO AG, Belfast, Milpitas, Kehlheim, pp 1–14

19.

Sims PJ, Waggoner AS, Wang CH, Hoffman JF (1974) Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. Biochemistry 13:3315–3330CrossRef

20.

Haugland RP (2002) Handbook of fluorescent probes and research products. Molecular Probes, Eugene

21.

Huser J, Lipp P, Niggli E (1996) Confocal microscopic detection of potential-sensitive dyes used to reveal loss of voltage control during patch-clamp experiments. Pflugers Arch 433:194–199CrossRef

22.

Lipp P, Huser J, Pott L, Niggli E (1996) Spatially non-uniform Ca²⁺ signals induced by the reduction of transverse tubules in citrate-loaded guinea-pig ventricular myocytes in culture. J Physiol 497(Pt 3):589–597

23.

Dibb KM, Clarke JD, Horn MA, Richards MA, Graham HK, Eisner DA, Trafford AW (2009) Characterization of an extensive transverse tubular network in sheep atrial myocytes and its depletion in heart failure. Circ Heart Fail 2:482–489CrossRef

24.

Sill B, Hammer PE, Cowan DB (2009) Optical mapping of Langendorff-perfused rat hearts. J Vis Exp. doi:10.3791/1138

25.

Loew LM (1996) Potentiometric dyes: imaging electrical activity of cell membranes. Pure Appl Chem 68:1405–1409CrossRef

26.

Bedlack RS Jr, Wei M, Loew LM (1992) Localized membrane depolarizations and localized calcium influx during electric field-guided neurite growth. Neuron 9:393–403CrossRef

27.

Gross E, Bedlack RS Jr, Loew LM (1994) Dual-wavelength ratiometric fluorescence measurement of the membrane dipole potential. Biophys J 67:208–216CrossRef

28.

Hardy ME, Lawrence CL, Standen NB, Rodrigo GC (2006) Can optical recordings of membrane potential be used to screen for drug-induced action potential prolongation in single cardiac myocytes? J Pharmacol Toxicol Meth 54:173–182CrossRef

29.

Kaestner L, Tian Q, Lipp P (2011) Cardiac Safety Screens: Molecular, cellular and optical advancements. In: Lin C P, Ntziachistos V (eds) SPIE biomedical optics III, vol. 8089, SPIE, Munich, in press

30.

Kuhn B, Fromherz P, Denk W (2004) High sensitivity of Stark-shift voltage-sensing dyes by one- or two-photon excitation near the red spectral edge. Biophys J 87:631–639CrossRef

31.

Fromherz P, Hubener G, Kuhn B, Hinner MJ (2008) ANNINE-6plus, a voltage-sensitive dye with good solubility, strong membrane binding and high sensitivity. Eur Biophys J 37:509–514CrossRef

32.

Hinner MJ, Hbener G, Fromherz P (2004) Enzyme-induced staining of biomembranes with voltage-sensitive fluorescent dyes. J Phys Chem B 108:2445–2453CrossRef

33.

Hinner MJ, Hubener G, Fromherz P (2006) Genetic targeting of individual cells with a voltage-sensitive dye through enzymatic activation of membrane binding. Chembiochem 7:495–505CrossRef

34.

Chanda B, Blunck R, Faria LC, Schweizer FE, Mody I, Bezanilla F (2005) A hybrid approach to measuring electrical activity in genetically specified neurons. Nat Neurosci 8:1619–1626CrossRef

35.

DiFranco M, Capote J, Quinonez M, Vergara JL (2007) Voltage-dependent dynamic FRET signals from the transverse tubules in mammalian skeletal muscle fibers. J Gen Physiol 130:581–600CrossRef

36.

Sjulson L, Miesenbock G (2008) Rational optimization and imaging in vivo of a genetically encoded optical voltage reporter. J Neurosci 28:5582–5593CrossRef

37.

Siegel MS, Isacoff EY (1997) A genetically encoded optical probe of membrane voltage. Neuron 19:735–741CrossRef

38.

Guerrero G, Siegel MS, Roska B, Loots E, Isacoff EY (2002) Tuning FlaSh: redesign of the dynamics, voltage range, and color of the genetically encoded optical sensor of membrane potential. Biophys J 83:3607–3618CrossRef

39.

Sakai R, Repunte-Canonigo V, Raj CD, Knopfel T (2001) Design and characterization of a DNA-encoded, voltage-sensitive fluorescent protein. Eur J Neurosci 13:2314–2318CrossRef

40.

Knopfel T, Tomita K, Shimazaki R, Sakai R (2003) Optical recordings of membrane potential using genetically targeted voltage-sensitive fluorescent proteins. Methods 30:42–48CrossRef

41.

Ataka K, Pieribone VA (2002) A genetically targetable fluorescent probe of channel gating with rapid kinetics. Biophys J 82:509–516CrossRef

42.

Baker BJ, Mutoh H, Dimitrov D, Akemann W, Perron A, Iwamoto Y, Jin L, Cohen LB, Isacoff EY, Pieribone VA, Hughes T, Knopfel T (2008) Genetically encoded fluorescent sensors of membrane potential. Brain Cell Biol 36:53–67CrossRef

43.

Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK (2007) Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells. J Neurosci Meth 161:32–38CrossRef

44.

Murata Y, Iwasaki H, Sasaki M, Inaba K, Okamura Y (2005) Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor. Nature 435:1239–1243CrossRef

45.

Ramsey IS, Moran MM, Chong JA, Clapham DE (2006) A voltage-gated proton-selective channel lacking the pore domain. Nature 440:1213–1216CrossRef

46.

Dimitrov D, He Y, Mutoh H, Baker BJ, Cohen L, Akemann W, Knopfel T (2007) Engineering and characterization of an enhanced fluorescent protein voltage sensor. PLoS ONE 2:e440CrossRef

47.

Tsutsui H, Karasawa S, Okamura Y, Miyawaki A (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat Methods 5:683–685CrossRef

48.

Lundby A, Akemann W, Knopfel T (2010) Biophysical characterization of the fluorescent protein voltage probe VSFP2.3 based on the voltage-sensing domain of Ci-VSP. Eur Biophys J 39:1625–1635CrossRef

49.

Akemann W, Mutoh H, Perron A, Rossier J, Knopfel T (2010) Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins. Nat Methods 7:643–649CrossRef

50.

Mutoh H, Perron A, Dimitrov D, Iwamoto Y, Akemann W, Chudakov DM, Knopfel T (2009) Spectrally-resolved response properties of the three most advanced FRET based fluorescent protein voltage probes. PLoS ONE 4:e4555CrossRef

51.

Gautam SG, Perron A, Mutoh H, Knopfel T (2009) Exploration of fluorescent protein voltage probes based on circularly permuted fluorescent proteins. Front Neuroeng 2:14CrossRef

52.

Perron A, Mutoh H, Launey T, Knopfel T (2009) Red-shifted voltage-sensitive fluorescent proteins. Chem Biol 16:1268–1277CrossRef

53.

Villalba-Galea CA, Sandtner W, Dimitrov D, Mutoh H, Knopfel T, Bezanilla F (2009) Charge movement of a voltage-sensitive fluorescent protein. Biophys J 96:L19–L21CrossRef

54.

Lundby A, Mutoh H, Dimitrov D, Akemann W, Knopfel T (2008) Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. PLoS ONE 3:e2514CrossRef

55.

Perron A, Mutoh H, Akemann W, Gautam SG, Dimitrov D, Iwamoto Y, Knopfel T (2009) Second and third generation voltage-sensitive fluorescent proteins for monitoring membrane potential. Front Mol Neurosci 2:5CrossRef

56.

Lundstrom K (2003) Semliki forest virus vectors for gene therapy. Expert Opin Biol Ther 3:771–777CrossRef

57.

Delenda C (2004) Lentiviral vectors: optimization of packaging, transduction and gene expression. J Gene Med 6(Suppl 1):S125–S138CrossRef

58.

Russell WC (2000) Update on adenovirus and its vectors. J Gen Virol 81:2573–2604

59.

Kaestner L, Ruppenthal S, Schwarz S, Scholz A, Lipp P (2009) Concepts for optical high content screens of excitable primary isolated cells for molecular imaging. In: Licha K, Lin CP (eds) SPIE biomedical optics, vol 7370. SPIE, Munich, pp 737–745

60.

Tallini YN, Ohkura M, Choi BR, Ji G, Imoto K, Doran R, Lee J, Plan P, Wilson J, Xin HB, Sanbe A, Gulick J, Mathai J, Robbins J, Salama G, Nakai J, Kotlikoff MI (2006) Imaging cellular signals in the heart in vivo: cardiac expression of the high-signal Ca²⁺ indicator GCaMP2. Proc Natl Acad Sci USA 103:4753–4758CrossRef

61.

Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR (2000) High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates. Circulation 101:178–184

62.

Buning H, Perabo L, Coutelle O, Quadt-Humme S, Hallek M (2008) Recent developments in adeno-associated virus vector technology. J Gene Med 10:717–733CrossRef

63.

Schultz BR, Chamberlain JS (2008) Recombinant adeno-associated virus transduction and integration. Mol Ther 16:1189–1199CrossRef

64.

Du L, Kido M, Lee DV, Rabinowitz JE, Samulski RJ, Jamieson SW, Weitzman MD, Thistlethwaite PA (2004) Differential myocardial gene delivery by recombinant serotype-specific adeno-associated viral vectors. Mol Ther 10:604–608CrossRef

65.

Schirmer JM, Miyagi N, Rao VP, Ricci D, Federspiel MJ, Kotin RM, Russell SJ, McGregor CG (2007) Recombinant adeno-associated virus vector for gene transfer to the transplanted rat heart. Transpl Int 20:550–557CrossRef

66.

Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J 381:307–312CrossRef

67.

Wlodarczyk J, Woehler A, Kobe F, Ponimaskin E, Zeug A, Neher E (2008) Analysis of FRET signals in the presence of free donors and acceptors. Biophys J 94:986–1000CrossRef

68.

Kaestner L, Lipp P (2009) Fluoreszenzproteine “fühlen” wo sie sind. BIOforum 32:17–18

69.

Valeur B (2002) Molecular fluorescence. Wiley-VCH, Winheim

70.

Tsutsui H, Higashijima S, Miyawaki A, Okamura Y (2010) Visualizing voltage dynamics in zebrafish heart. J Physiol 588:2017–2021CrossRef

71.

Asselberghs I, Flors C, Ferrighi L, Botek E, Champagne B, Mizuno H, Ando R, Miyawaki A, Hofkens J, Van der Auweraer M, Clays K (2008) Second-harmonic generation in GFP-like proteins. J Am Chem Soc 130:15713–15719CrossRef

72.

De Meulenaere E, Asselberghs I, de Wergifosse M, Botek E, Spaepen S, Champagne B, Vanderleyden J, Clays K (2009) Second-order nonlinear optical properties of fluorescent proteins for second-harmonic imaging. Journal of Materials Chemistry, 19:7514–7519

73.

Bublitz G, King B, Boxer S (1998) Electronic structure of the chromophore in green fluorescent protein. J Am Chem Soc 120:9370CrossRef

74.

Rosell FI, Boxer SG (2003) Polarized absorption spectra of green fluorescent protein single crystals: transition dipole moment directions. Biochemistry 42:177–183CrossRef

75.

Shi X, Basran J, Seward HE, Childs W, Bagshaw CR, Boxer SG (2007) Anomalous negative fluorescence anisotropy in yellow fluorescent protein (YFP 10C): quantitative analysis of FRET in YFP dimers. Biochemistry 46:14403–14417CrossRef

76.

Khatchatouriants A, Lewis A, Rothman Z, Loew L, Treinin M (2000) GFP is a selective non-linear optical sensor of electrophysiological processes in Caenorhabditis elegans. Biophys J 79:2345–2352CrossRef

77.

Topell S, Hennecke J, Glockshuber R (1999) Circularly permuted variants of the green fluorescent protein. FEBS Lett 457:283–289CrossRef

78.

Baird GS, Zacharias DA, Tsien RY (1999) Circular permutation and receptor insertion within green fluorescent proteins. Proc Natl Acad Sci USA 96:11241–11246CrossRef

79.

Kaestner L, Lipp P (2007) Non-linear and ultra high-speed imaging for explorations of the murine and human heart. In: Popp J, von Bally G (eds) Optics in life science, vol 6633. SPIE, Munich, pp 66330K-1–66330K-10

80.

Shimomura O, Johnson FH, Saiga Y (1962) Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol 59:223–239CrossRef

81.

Tasaki I, Watanabe A, Sandlin R, Carnay L (1968) Changes in fluorescence, turbidity, and birefringence associated with nerve excitation. Proc Natl Acad Sci USA 61:883–888CrossRef

82.

Heim R, Tsien RY (1996) Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol 6:178–182CrossRef

83.

Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ (1992) Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111:229–233CrossRef

84.

Zimmer M (2005) Glowing genes: a revolution in biotechnology. Prometheus Books, Amherst

85.

Fluhler E, Burnham VG, Loew LM (1985) Spectra, membrane binding, and potentiometric responses of new charge shift probes. Biochemistry 24:5749–5755CrossRef

86.

Salzberg BM, Grinvald A, Cohen LB, Davila HV, Ross WN (1977) Optical recording of neuronal activity in an invertebrate central nervous system: simultaneous monitoring of several neurons. J Neurophysiol 40:1281–1291

87.

Grinvald A, Salzberg BM, Cohen LB (1977) Simultaneous recording from several neurones in an invertebrate central nervous system. Nature 268:140–142CrossRef

88.

Salzberg BM, Davila HV, Cohen LB (1973) Optical recording of impulses in individual neurones of an invertebrate central nervous system. Nature 246:508–509CrossRef

Titel: Action Potentials in Heart Cells
verfasst von: Lars Kaestner
Qinghai Tian
Peter Lipp
Verlag: Springer Berlin Heidelberg
Buch: Fluorescent Proteins II
Print ISBN: 978-3-642-23376-0

Electronic ISBN: 978-3-642-23377-7

Copyright-Jahr: 2012
DOI: https://doi.org/10.1007/4243_2011_28

Premium Partner

Marktübersichten

Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen.

Zur Marktübersicht