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Like with chemical catalysts, a few guidelines should be observed with biocatalysts to preserve activity and to handle them safely. Depending on the formulation – liquid, lyophilized, spray-dried, immobilized – different rules apply. Enzymes are often perceived as unstable and delicate entities, however, when treated in the right way, they can be as sturdy as almost any chemical catalyst. For optimal stability, enzyme preparations are usually stored best in their original commercial form, either as lyophilizate, spray-dried powder or (stabilized) liquid.
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SP525 is a powder containing about 40% of weight protein, while SP435 is the same enzyme immobilized on macroporous polypropylene and contains about 1% of weight protein. For some time, Novo supplied a lipase SP382, which was a mixture of lipases A and B.
The amino acid sequences of lipases from Rhizopus delemar, R. javanicus and R. niveus are identical. The sequence of the lipase from R. oryzae differs by only two conservative substitutions.
The amino acid sequence and biochemical properties of the lipases from Pseudomonas glumae and Chromobacterium viscosum are identical; see: Taipa MA, Liebeton K, Costa JV, Cabral JMS, Jaeger KE (1995) Biochim. Biophys. Acta 1256: 395; Lang D, Hofmann B, Haalck L, Hecht HJ, Spener F, Schmid RD, Schomburg D (1996) J. Mol. Biol. 259: 704.
Prof. Dr. Kurt Faber
- Springer Berlin Heidelberg
- Chapter 5