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Erschienen in:

2015 | Online First | Buchkapitel

Applying Shear Stress to Pluripotent Stem Cells

verfasst von : Russell P. Wolfe, Julia B. Guidry, Stephanie L. Messina, Tabassum Ahsan

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

Thorough understanding of the effects of shear stress on stem cells is critical for the rationale design of large-scale production of cell-based therapies. This is of growing importance as emerging tissue engineering and regenerative medicine applications drive the need for clinically relevant numbers of both pluripotent stem cells (PSCs) and cells derived from PSCs. Here, we describe the use of a custom parallel plate bioreactor system to impose fluid shear stress on a layer of PSCs adhered to protein-coated glass slides. This system can be useful both for basic science studies in mechanotransduction and as a surrogate model for bioreactors used in large-scale production.
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Metadaten
Titel
Applying Shear Stress to Pluripotent Stem Cells
verfasst von
Russell P. Wolfe
Julia B. Guidry
Stephanie L. Messina
Tabassum Ahsan
Copyright-Jahr
2015
Verlag
Springer New York
DOI
https://doi.org/10.1007/7651_2015_210