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15.04.2016 | Original Paper

Assessment of endoglucanase activity by analyzing the degree of cellulose polymerization and high-throughput analysis by near-infrared spectroscopy

verfasst von: Yoshiki Horikawa, Tomoya Imai, Kentaro Abe, Keita Sakakibara, Yoshinobu Tsujii, Asako Mihashi, Yoshinori Kobayashi, Junji Sugiyama

Erschienen in: Cellulose | Ausgabe 3/2016

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Abstract

We developed a novel and practical assessment technique for endoglucanase (EG) activity by measuring the degree of polymerization (DP) of cellulose from Eucalyptus globulus. This evaluation method demonstrated that EG II from Trichoderma reesei had higher endoglucanase activity than EG I, which has not been detected in conventional experiments using carboxymethyl cellulose as a model substrate. In addition, a high-throughput protocol for DP measurement was developed by using near-infrared spectroscopy combined with a multivariate analysis. Interpreting the regression coefficient, a reciprocal correlation was observed between the relative crystallinity of the cellulosic residue after enzymatic hydrolysis and the DP.

Vorheriger Artikel Chemically peeling layers of cellulose nanocrystals by periodate and chlorite oxidation

Nächster Artikel Coarse-grained model of the native cellulose and the transformation pathways to the allomorph

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Titel: Assessment of endoglucanase activity by analyzing the degree of cellulose polymerization and high-throughput analysis by near-infrared spectroscopy
verfasst von: Yoshiki Horikawa
Tomoya Imai
Kentaro Abe
Keita Sakakibara
Yoshinobu Tsujii
Asako Mihashi
Yoshinori Kobayashi
Junji Sugiyama
Publikationsdatum: 15.04.2016
Verlag: Springer Netherlands
Erschienen in: Cellulose / Ausgabe 3/2016
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-016-0927-9

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