The application of low-cost feedstock and optimizations as well as variables that influence the income aids of biobutanol is investigated. This research studies the influence concentration of ZnO nanoparticle as a catalyst on the trans-esterification of mango (Mangifera indica) seed bio-oil with methanols by means of KOH as catalysts. A yield of 85% wt was attained at optimal temperatures of 50 \(^\circ{\rm C}\). A comparable tendency was perceived on the influence of reagent concentrations, with the optimal being 85%wt at 10 mL of ZnO nanocatalyst. The performances and emission parameters, including BTE, BSFC, CO, UHC, NOx, and CO2, were also evaluated. The highest BTE for the base diesel are achieved at full load in the following circumstances: DE10, DE20, DE30, DE40, and DE50 are 27.4%, 27.3%, 17.7%, 20.3%, 20.2%, and 24.7%, respectively. BSFC of base diesel indicates that DE10, DE20, DE30, DE40, and DE50 was 0.77, 2.1, 1.61, 4.5, 4.7, and 2.6 kg/kWh, respectively. The exhaust gas temperature (EGT) of DE10 is greater than base diesel. There was a 10% reduction in HC emissions, indicating that the biodiesel blend for DE10 burns more efficiently. The decrease in carbon-monoxide releases for DE10, DE20, DE30, DE40 and DE50 are 0.24%, 0.25%, 0.28%, 0.29%, and 0.22%, respectively. NOx emanation of DE10 is greater than before by 2% in comparison to diesel at the esteemed loads, and the oxide of nitrogen (NOx) emanation of DE100 is increased by 20% as a result of the proportion of biodiesel in the blend. The variance of carbon dioxide (CO2) emanation with reverence to brake-power for base-diesel, DE10, DE20, DE30, DE40, and DE50, are 5.9%, 5.0%, 5.9%, 5.57%, 5.8%, and 5.2%, respectively. Exploration of the biodiesel created presented uniformity with the beginning customary value cited by ASTM and EN for biodiesel as well as fossil diesel. This indicates that the biodiesel created from mango seed extracts is of highly performed qualities and applied to combine the fossils grounded diesel.