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Journal of Materials Science

Erschienen in:

03.12.2018 | Materials for life sciences

Bioinformatics and enzymatics investigation of Trametes laccase for optical biosensing application

verfasst von: Yang Wang, Zhi-Hui Chen

Erschienen in: Journal of Materials Science | Ausgabe 6/2019

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Abstract

Laccase has recently drawn a considerable attention as a promising tool for optical biosensing application, mainly due to its attractive intrinsic optical properties. The discovery of a laccase with great enzyme stability is the principal step for enzyme-based optical biosensing application in in vitro environments. In this paper, we found that Trametes sp. SQ1 laccase retained more than 150% activity after storage in different conditions for 96 h, and the laccase activity was not affected by freeze–thaw. The reduced form of enzyme showed a new maximal increase in absorbance peak at 400 nm, while the fluorescence intensity of the oxidized form of enzyme was much stronger than that of the reduced enzyme. Therefore, Trametes sp. SQ1 laccase with good stability and optical properties will be a competitive candidate for optical biosensing application. Moreover, bioinformatics analysis on Trametes laccases revealed that the enzymes may be modified by N-glycosylation and have intermolecular disulfide bond for forming functional oligomer, which plays a role in stabilizing enzyme function. The results indicate that Trametes laccases are able to be with good stability. It will provide a guide in searching stable laccases for optical biosensing application.

Vorheriger Artikel Biomacromolecules in bivalve shells with crossed lamellar architecture

Nächster Artikel The strengthening of woven jute fiber/polylactide biocomposite without loss of ductility using rigid core–soft shell nanoparticles

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Titel: Bioinformatics and enzymatics investigation of Trametes laccase for optical biosensing application
verfasst von: Yang Wang
Zhi-Hui Chen
Publikationsdatum: 03.12.2018
Verlag: Springer US
Erschienen in: Journal of Materials Science / Ausgabe 6/2019
Print ISSN: 0022-2461
Elektronische ISSN: 1573-4803
DOI: https://doi.org/10.1007/s10853-018-03187-9

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