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2015 | OriginalPaper | Buchkapitel

17. Catgut Waste Utilization for Protease Production Using Bacillus subtilis

verfasst von : Jerusha Kezia Clement, Raja Mohan, Jegannathan Kenthorai Raman

Erschienen in: Advances in Bioprocess Technology

Verlag: Springer International Publishing

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Abstract

Industrial enzymes represent the heart of biotechnology processes and utilization of industrial waste for enzyme production could make the enzymes cheap and process sustainable. In this study, catgut waste was used as a novel substrate for protease production using Bacillus subtilis. Catgut is an absorbable surgical suture obtained from the animal tissue containing >97 % pure bovine serosa. Catgut waste finely ground to powder was supplied to Bacillus subtilis as a substrate to produce protease enzyme in a submerged fermentation. The process conditions such as pH, substrate concentration, inoculum level, inoculum age, temperature, duration and mixing intensity were optimized using Plackett Burman design and Response Surface Methodology. The maximum protease activity was found to be 111.36 U/ml at optimum process condition level of 4 % substrate concentration, 5 % inoculum level, 18 h inoculum age, pH 7, 140 rpm, 40 °C and 96 h incubation period.

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Titel: Catgut Waste Utilization for Protease Production Using Bacillus subtilis
verfasst von: Jerusha Kezia Clement
Raja Mohan
Jegannathan Kenthorai Raman
Verlag: Springer International Publishing
Buch: Advances in Bioprocess Technology
Print ISBN: 978-3-319-17914-8

Electronic ISBN: 978-3-319-17915-5

Copyright-Jahr: 2015
DOI: https://doi.org/10.1007/978-3-319-17915-5_17