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Erschienen in:
2012 | OriginalPaper | Buchkapitel
Cell Tracking for Automatic Migration and Proliferation Analysis in High-Throughput Screens
verfasst von : Nathalie Harder, Richa Batra, Sina Gogolin, Nicolle Diessl, Roland Eils, Frank Westermann, Rainer König, Karl Rohr
Erschienen in: Bildverarbeitung für die Medizin 2012
Verlag: Springer Berlin Heidelberg
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Systematic analysis of cell migration and proliferation in large-scale image-based screens is important for the final evaluation as well as for optimization of experimental conditions. We here present a tracking approach for high-throughput image data, and extract global movement and growth patterns on a large scale. In particular, we analyze between-spot movement on cell arrays to estimate the confidence of the phenotypic readout, we determine the cellular reproduction time, and we automatically detect multi-polar divisions to support the identification of target genes. A quantitative evaluation of our approach showed that high segmentation and tracking accuracies of 92% and 98% are reached.