Skip to main content
Erschienen in: Journal of Visualization 3/2014

01.08.2014 | Regular Paper

Cellular imaging using phase holographic microscopy: for the study of pathophysiology of red blood cells and human umbilical vein endothelial cells

verfasst von: Kyung Won Seo, Eunseok Seo, Sang Joon Lee

Erschienen in: Journal of Visualization | Ausgabe 3/2014

Einloggen

Aktivieren Sie unsere intelligente Suche um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

Digital holographic microscopy (DHM) for phase-contrast imaging is employed for live cell imaging to study pathophysiology of red blood cells (RBCs) and human umbilical vein endothelial cells (HUVECs). The morphology and membrane fluctuation of healthy RBCs are investigated by adapting post-image processing which automatically quantify the morphological properties of RBCs, such as perimeter, mean diameter, cell volume, cell thickness and membrane fluctuation. Moreover, morphological variation of malaria parasite-infected RBCs and hypertonic-shocked RBCs was observed. As another biological application, HUVECs cultured on collagen films and their abnormal deformations caused by collagenase are observed using DHM to understand the role of extracellular matrix (ECM) structure on atheroprotective effect and endothelial dysfunction.

Graphical Abstract

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Springer Professional "Wirtschaft"

Online-Abonnement

Mit Springer Professional "Wirtschaft" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 340 Zeitschriften

aus folgenden Fachgebieten:

  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Versicherung + Risiko




Jetzt Wissensvorsprung sichern!

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Literatur
Zurück zum Zitat Byun H, Hillman TR, Higgins JM, Diez-Silva M, Peng ZL, Dao M et al (2012) Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient. Acta Biomater 8:8–4130CrossRef Byun H, Hillman TR, Higgins JM, Diez-Silva M, Peng ZL, Dao M et al (2012) Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient. Acta Biomater 8:8–4130CrossRef
Zurück zum Zitat Cuche E, Marquet P, Depeursinge C (1999) Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. Appl Opt 38:6994–7001CrossRef Cuche E, Marquet P, Depeursinge C (1999) Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. Appl Opt 38:6994–7001CrossRef
Zurück zum Zitat Herraez MA, Burton DR, Lalor MJ, Gdeisat MA (2002) Fast two-dimensional phase-unwrapping algorithm based on sorting by reliability following a noncontinuous path. Appl Opt 41:7437–7444CrossRef Herraez MA, Burton DR, Lalor MJ, Gdeisat MA (2002) Fast two-dimensional phase-unwrapping algorithm based on sorting by reliability following a noncontinuous path. Appl Opt 41:7437–7444CrossRef
Zurück zum Zitat Kemper B, Bauwens A, Vollmer A, Ketelhut S, Langehanenberg P, Muthing J et al (2010) Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy. J Biomed Opt 15:036009CrossRef Kemper B, Bauwens A, Vollmer A, Ketelhut S, Langehanenberg P, Muthing J et al (2010) Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy. J Biomed Opt 15:036009CrossRef
Zurück zum Zitat Mann CJ, Yu LF, Lo CM, Kim MK (2005) High-resolution quantitative phase-contrast microscopy by digital holography. Opt Express 13:8693–8698CrossRef Mann CJ, Yu LF, Lo CM, Kim MK (2005) High-resolution quantitative phase-contrast microscopy by digital holography. Opt Express 13:8693–8698CrossRef
Zurück zum Zitat Marquet P, Rappaz B, Magistretti PJ, Cuche E, Emery Y, Colomb T et al (2005) Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. Opt Lett 30:468–470CrossRef Marquet P, Rappaz B, Magistretti PJ, Cuche E, Emery Y, Colomb T et al (2005) Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. Opt Lett 30:468–470CrossRef
Zurück zum Zitat McLaren CE, Brittenham GM, Hasselblad V (1987) Statistical and graphical evaluation of erythrocyte volume distributions. Am J Physiol 252:H857–H866 McLaren CE, Brittenham GM, Hasselblad V (1987) Statistical and graphical evaluation of erythrocyte volume distributions. Am J Physiol 252:H857–H866
Zurück zum Zitat Nomarski G (1955) Differential microinterferometer with polarized waves. J Phys Radium 16:6–13 Nomarski G (1955) Differential microinterferometer with polarized waves. J Phys Radium 16:6–13
Zurück zum Zitat Park YK, Diez-Silva M, Popescu G, Lykotrafitis G, Choi WS, Feld MS et al (2008) Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum. Proc Natl Acad Sci USA 105:13730–13735CrossRef Park YK, Diez-Silva M, Popescu G, Lykotrafitis G, Choi WS, Feld MS et al (2008) Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum. Proc Natl Acad Sci USA 105:13730–13735CrossRef
Zurück zum Zitat Park Y, Best CA, Badizadegan K, Dasari RR, Feld MS, Kuriabova T et al (2010) Measurement of red blood cell mechanics during morphological changes. Proc Natl Acad Sci USA 107:6731–6736CrossRef Park Y, Best CA, Badizadegan K, Dasari RR, Feld MS, Kuriabova T et al (2010) Measurement of red blood cell mechanics during morphological changes. Proc Natl Acad Sci USA 107:6731–6736CrossRef
Zurück zum Zitat Park Y, Best CA, Kuriabova T, Henle ML, Feld MS, Levine AJ et al (2011) Measurement of the nonlinear elasticity of red blood cell membranes. Phys Review E 83:051925CrossRef Park Y, Best CA, Kuriabova T, Henle ML, Feld MS, Levine AJ et al (2011) Measurement of the nonlinear elasticity of red blood cell membranes. Phys Review E 83:051925CrossRef
Zurück zum Zitat Popescu G, Ikeda T, Best CA, Badizadegan K, Dasari RR, and Feld MS (2005) Erythrocyte structure and dynamics quantified by Hilbert phase microscopy. J Biomed Opt 10 Popescu G, Ikeda T, Best CA, Badizadegan K, Dasari RR, and Feld MS (2005) Erythrocyte structure and dynamics quantified by Hilbert phase microscopy. J Biomed Opt 10
Zurück zum Zitat Popescu G, Ikeda T, Dasari RR, Feld MS (2006) Diffraction phase microscopy for quantifying cell structure and dynamics. Opt Lett 31:7–775CrossRef Popescu G, Ikeda T, Dasari RR, Feld MS (2006) Diffraction phase microscopy for quantifying cell structure and dynamics. Opt Lett 31:7–775CrossRef
Zurück zum Zitat Popescu G, Park Y, Choi W, Dasari RR, Feld MS, Badizadegan K (2008) Imaging red blood cell dynamics by quantitative phase microscopy. Blood Cells Mol Dis 41:10–16CrossRef Popescu G, Park Y, Choi W, Dasari RR, Feld MS, Badizadegan K (2008) Imaging red blood cell dynamics by quantitative phase microscopy. Blood Cells Mol Dis 41:10–16CrossRef
Zurück zum Zitat Rappaz B, Marquet P, Cuche E, Emery Y, Depeursinge C, Magistretti PJ (2005) Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy. Opt Express 13:9361–9373CrossRef Rappaz B, Marquet P, Cuche E, Emery Y, Depeursinge C, Magistretti PJ (2005) Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy. Opt Express 13:9361–9373CrossRef
Zurück zum Zitat Seo KW, Choi YS, Seo ES, Lee SJ (2012) Aberration compensation for objective phase curvature in phase holographic microscopy. Opt Lett 37:8–4976CrossRef Seo KW, Choi YS, Seo ES, Lee SJ (2012) Aberration compensation for objective phase curvature in phase holographic microscopy. Opt Lett 37:8–4976CrossRef
Zurück zum Zitat Yamaguchi I, Zhang T (1997) Phase-shifting digital holography. Opt Lett 22:1268–1270CrossRef Yamaguchi I, Zhang T (1997) Phase-shifting digital holography. Opt Lett 22:1268–1270CrossRef
Zurück zum Zitat Yu LF, Kim MK (2005) Wavelength-scanning digital interference holography for tomographic three-dimensional imaging by use of the angular spectrum method. Opt Lett 30:2092–2094CrossRef Yu LF, Kim MK (2005) Wavelength-scanning digital interference holography for tomographic three-dimensional imaging by use of the angular spectrum method. Opt Lett 30:2092–2094CrossRef
Zurück zum Zitat Zernike F (1942a) Phase contrast, a new method for the microscopic observation of transparent objects. Physica 9:686–698CrossRef Zernike F (1942a) Phase contrast, a new method for the microscopic observation of transparent objects. Physica 9:686–698CrossRef
Zurück zum Zitat Zernike F (1942b) Phase contrast, a new method for the microscopic observation of transparent objects part II. Physica 9:974–980CrossRef Zernike F (1942b) Phase contrast, a new method for the microscopic observation of transparent objects part II. Physica 9:974–980CrossRef
Zurück zum Zitat Zhang T, Yamaguchi I (1998) Three-dimensional microscopy with phase-shifting digital holography. Opt Lett 23:3–1221 Zhang T, Yamaguchi I (1998) Three-dimensional microscopy with phase-shifting digital holography. Opt Lett 23:3–1221
Metadaten
Titel
Cellular imaging using phase holographic microscopy: for the study of pathophysiology of red blood cells and human umbilical vein endothelial cells
verfasst von
Kyung Won Seo
Eunseok Seo
Sang Joon Lee
Publikationsdatum
01.08.2014
Verlag
Springer Berlin Heidelberg
Erschienen in
Journal of Visualization / Ausgabe 3/2014
Print ISSN: 1343-8875
Elektronische ISSN: 1875-8975
DOI
https://doi.org/10.1007/s12650-014-0200-y

Weitere Artikel der Ausgabe 3/2014

Journal of Visualization 3/2014 Zur Ausgabe