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Doubly-Labeled Water Studies of Vertebrate Physiological Ecology

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Stable Isotopes in Ecological Research

Part of the book series: Ecological Studies ((ECOLSTUD,volume 68))

Abstract

Doubly-labeled water (DLW) is water containing enriched levels of stable or radioactive isotopes of hydrogen and oxygen. The hydrogen isotopes that have been used are tritium, which is radioactive and is denoted as 3H, H-3, or T, and deuterium, which is stable and is denoted as 2H, H-2, or D. Oxygen-18 (18O or O-18) is the oxygen isotope of choice. Oxygen-17 could be used but adequate measurement technology for its use in DLW studies on animals has not yet been developed.

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References

  • Bickler PE and Nagy KA (1980) Effects of parietalectomy on energy expenditure in free-ranging lizards. Copeia 1980:923–925.

    Article  Google Scholar 

  • Buscarlet LA and Proux J (1975) Etude à Taide de l’eau tritiée du renouvellement de l’eau corporelle chez Locusta migratoria migratorioides. C.R. Acad. Sci. Paris Ser. D 281:1409–1412.

    Google Scholar 

  • Buscarlet LA, Proux J, and Gerster R (1978) Utilisation du double marquage HT18O dans une étude de bilan metabolique chez Locusta migratoria migratorioides. J. Insect. Physiol. 24:225–232.

    Article  CAS  Google Scholar 

  • Buttemer WA, Hay worth AM, Weathers WW, and Nagy KA (1986) Time-budget es-timates of avian energy expenditure:physiological and meteorlogical considerations. Physiol. Zool. 59:131–149.

    Google Scholar 

  • Congdon JD, King WW, and Nagy KA (1978) Validation of the HTO-18 method for determination of CO2 production of lizards (genus Sceloporus). Copeia 1978:360– 362.

    Google Scholar 

  • Cooper PD (1983) Validation of the doubly labeled water (H3H18O) method for measuring water flux and energy metabolism in tenebrionid beetles. Physiol. Zool. 56:41–46.

    Google Scholar 

  • Coward WA and Prentice AM (1985) Isotope method for the measurement of carbon dioxide production rate in man. Am. J. Clin. Nutr. 41:659–660.

    PubMed  CAS  Google Scholar 

  • Feinendegen LE (1967) Tritium-Labeled Molecules in Biology and Medicine. Academic Press, New York, p. 2.

    Google Scholar 

  • Flint EN and Nagy KA (1984) Flight energetics of free-living sooty terns. Auk 101:288– 294.

    Google Scholar 

  • Gettinger RD (1983) Use of doubly-labeled water (3HH18O) for determination of H2O flux and CO2 production by a mammal in a humid environment. Oecologia 59:54– 57.

    Google Scholar 

  • Goldstein DL and Nagy KA (1985) Resource utilization by desert quail:time and energy, food and water. Ecology 66:378–387.

    Article  Google Scholar 

  • Hails CJ (1979) A comparison of flight energetics in hirundines and other birds. Comp. Biochem. Physiol. 63A:581–585.

    Google Scholar 

  • Karasov WH (1981) Daily energy expenditure and the cost of activity in a free-living mammal. Oecologia 51:253–259.

    Article  Google Scholar 

  • Karasov WH (1983) Wintertime energy conservation by huddling in antelope ground squirrels (Ammospermophilus leucurus). J. Mammal. 64:341–345.

    Article  Google Scholar 

  • King JM and Finch VA (1982) Value and limitations of using tritiated water for predicting body composition and water intake in the African Zebu. pp. 57–67. In Use of Tritiated Water in Studies of Production and Adaptation in Ruminants. International Atomic Energy Agency, Vienna.

    Google Scholar 

  • King WW and Hadley NF (1979) Water flux and metabolic rates of free-roaming scorpions using the doubly labeled water technique. Physiol. Zool. 52:176–189.

    Google Scholar 

  • Klein PD, James WPT, Wong WW, Irving CS, Murgatroyd PR, Cabrera M, Dallosso HM, Klein ER, and Nichols BL (1984) Calorimetric validation of the doubly-labeled water method for determination of energy expenditure in man. Hum. Nutr. Clin. Nutr. 38C:95–106.

    CAS  Google Scholar 

  • Lee JS and Lifson N (1960) Measurement of total energy and material balance in rats by means of doubly labeled water. Am. J. Physiol. 199:238–242.

    PubMed  CAS  Google Scholar 

  • LeFebvre EA (1964) The use of D218O for measuring energy metabolism in Columba livia at rest in flight. Auk 81:403–416.

    Google Scholar 

  • Lifson N, Gordon GB, and McClintock R (1955) Measurement of total carbon dioxide production by means of D218O. J. Appl. Physiol. 7:704–710.

    PubMed  CAS  Google Scholar 

  • Lifson N, Gordon GB, Visscher MB, and Nier AO (1949) The fate of utilized molecular oxygen of respiratory carbon dioxide, studied with the aid of heavy oxygen. J. Biol. Chem. 180:803–811.

    PubMed  CAS  Google Scholar 

  • Lifson N and Lee JS (1961) Estimation of material balance of totally fasted rats by doubly labeled water. Am. J. Physiol. 200:85–88.

    PubMed  CAS  Google Scholar 

  • Lifson N, Little WS, Levitt DG, and Henderson RM (1975) D218O method for CO2 out-put in small mammals and economic feasibility in man. J. Appl. Physiol. 39:657– 664.

    Google Scholar 

  • Lifson N and McClintock R (1966) Theory of use of the turnover rates of body water for measuring energy and material balance. J. Theor. Biol. 12:46–74.

    Article  PubMed  CAS  Google Scholar 

  • Little WW and Lifson N (1975) Validation study of D218O method for determination of CO2 output of the eastern chipmunk (Tamias striatus). Comp. Biochem. Physiol. A50. 55–56.

    Google Scholar 

  • McClintock R and Lifson N (1957) Applicability of the D218O method to the measurement of the total carbon dioxide output of obese mice. J. Biol. Chem. 226:153–156.

    PubMed  CAS  Google Scholar 

  • Mullen RK (1970) Respiratory metabolism and body water turnover rates of Perognathus formosus in its natural environment. Comp. Biochem. Physiol. 32:259–265.

    Article  CAS  Google Scholar 

  • Nagy KA (1975) Water and energy budgets of free-living animals:measurement using isotopically labeled water, pp. 227–245. In Hadley NF (editor), Environmental Phys-iology of Desert Organisms. Dowden, Hutchison and Ross, Stroudsburg, Pennsylvania.

    Google Scholar 

  • Nagy KA (1980) CO2 production in animals:analysis of potential errors in the doubly labeled water method. Am. J. Physiol. 238:R466–R473.

    PubMed  CAS  Google Scholar 

  • Nagy KA (1982) Energy requirements of free-living iguanid lizards, pp. 49–59. In Burghardt GM and Rand AS (editors), Iguanas of the World:Their Behavior, Ecology, and Conservation. Noyes Publications, Park Ridge, New Jersey.

    Google Scholar 

  • Nagy KA (1983a) The doubly labeled water (3HH18O) method:a guide to its use. UCLA Publ. 12–1417.

    Google Scholar 

  • Nagy KA (1983b) Ecological energetics, pp. 24–54. In Huey RB, Pianka ER, and Schoe- ner TW (editors), Lizard Ecology:Studies of a Model Organism. Harvard University Press, Cambridge.

    Google Scholar 

  • Nagy KA (1987). Field metabolic rate and food requirement scaling in mammals and birds. Ecol. Monogr. 57:111–128.

    Article  Google Scholar 

  • Nagy KA (1986) Physiological ecology of desert tortoises in southern Nevada. Herpetol 42:73–92.

    Google Scholar 

  • Nagy KA and Costa DP (1980) Water flux in animals:analysis of potential errors in the tritiated water method. Am. J. Physiol. 238:R454–R465.

    PubMed  CAS  Google Scholar 

  • Nagy KA, Huey RB, and Bennett AF (1984a) Field energetics and foraging mode of Kalahari lacertid lizards. Ecology 65:588–596.

    Article  Google Scholar 

  • Nagy KA and Milton K (1979) Energy metabolism and food consumption by wild howler monkeys (Alouatta palliata). Ecology 60:475–480.

    Article  Google Scholar 

  • Nagy KA and Shoemaker VH (1984) Field energetics and food consumption of the Galapagos marine iguana, Amblyrhynchus cristatus. Physiol. Zool. 57:281–290.

    Google Scholar 

  • Nagy KA, Siegfried WR, and Wilson RP (1984b) Energy utilization by free-ranging jackass penguins, Spheniscus demersus. Ecology 65:1648–1655.

    Article  Google Scholar 

  • Pinson EA (1952) Water exchange and barriers as studied by the use of hydrogen isotopes. Physiol. Rev. 32:123–234.

    CAS  Google Scholar 

  • Roberts SB, Coward WA, Schlingenseipen K-H, Nohria V, and Lucas A (1986) Comparison of the doubly labeled water (2H218O) method with indirect calorimetry and a nutrient-balance study for simultaneous determination of energy expenditure, water intake, and metabolizable energy intake in preterm infants. Am. J. Clin. Nutr. 44:315– 322.

    Google Scholar 

  • Schoeller DA (1983) Energy expenditure from doubly labeled water:some considerations in humans. Am. J. Clin. Nutr. 38:999–1005.

    PubMed  CAS  Google Scholar 

  • Schoeller DA, Dietz W, van Santen E, and Klein PD (1982) Validation of saliva sampling for total body water determination by H218O dilution. Am. J. Clin. Nutr. 35:591–594.

    PubMed  CAS  Google Scholar 

  • Schoeller DA, Kushner RF, and Jones PJH (1986) Validation of doubly labeled water for measuring energy expenditure during parenteral nutrition. Am. J. Clin. Nutr. 44:291–298.

    PubMed  CAS  Google Scholar 

  • Schoeller DA, van Santen E, Peterson DW, Dietz W, Jaspan J, and Klein PD (1980) Total body water measurement in humans with 18O and 2H labeled water. Am. J. Clin. Nutr. 33:2686–2693.

    PubMed  CAS  Google Scholar 

  • Schoeller DA and van Santen E (1982) Measurement of energy expenditure in humans by doubly labeled water method. J. Appl. Physiol. 53:955–959.

    PubMed  CAS  Google Scholar 

  • Schoeller DA and Webb P (1984) Five-day comparison of the doubly labeled water method with respiratory gas exchange. Am. J. Clin. Nutr. 40:153–158.

    PubMed  CAS  Google Scholar 

  • Shoemaker VH and Nagy (1984) Osmoregulation of the Galapagos marine iguana, Amblyrhynchus cristatus. Physiol. Zool. 57:291–300.

    Google Scholar 

  • Stansell MJ and Mojica L (1968) Determination of body water content using trace levels of deuterium oxide and infrared spectrophotometry. Clin. Chem. 14:1112–1124.

    CAS  Google Scholar 

  • Turner MD, Neely WA, and Hardy JD (1960) Rapid determination of deuterium oxide in biological fluids. J. Appl. Physiol. 15:309–310.

    PubMed  CAS  Google Scholar 

  • Utter JM and LeFebvre EA (1973) Daily energy expenditure of purple martins (Progne subis) during the breeding season:estimates using D2O18 and time budget methods. Ecology 54:597–604.

    Article  Google Scholar 

  • Westerterp KR, de Boer JO, Saris WHM, Schoffelen PFM, and ten Hoor F (1984) Measurement of energy expenditure using doubly labeled water. Int. J. Sports Med. 5 (supplement):74–75.

    Article  Google Scholar 

  • Williams JB (1985) Validation of the doubly labeled water technique for measuring energy metabolism in starlings and sparrows. Comp. Biochem. Physiol. 80A:349–353.

    Google Scholar 

  • Williams JB and Nagy KA (1984) Validation of the doubly labeled water technique for measuring energy metabolism in savannah sparrows. Physiol. Zool. 57:325–328.

    Google Scholar 

  • Wood RA, Nagy KA, MacDonald NS, Wakakuwa ST, Beckman RJ, and Kaaz H (1975) Determination of oxygen-18 in water contained in biological samples by charged particle activation. Anal. Chem. 47:646–650.

    CAS  Google Scholar 

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Authors
  1. K. A. Nagy
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Editor information

Editors and Affiliations

  1. Laboratory of Biomedical and Environmental Sciences, University of California, 90024, Los Angeles, CA, USA

    P. W. Rundel  & K. A. Nagy  & 

  2. Department of Biology, University of Utah, 84112, Salt Lake City, UT, USA

    J. R. Ehleringer

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© 1989 Springer-Verlag New York Inc.

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Nagy, K.A. (1989). Doubly-Labeled Water Studies of Vertebrate Physiological Ecology. In: Rundel, P.W., Ehleringer, J.R., Nagy, K.A. (eds) Stable Isotopes in Ecological Research. Ecological Studies, vol 68. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3498-2_16

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  • DOI: https://doi.org/10.1007/978-1-4612-3498-2_16

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-8127-6

  • Online ISBN: 978-1-4612-3498-2

  • eBook Packages: Springer Book Archive

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