Abstract
The human cornea is the transparent surface of the eye, which serves as the main refractive element of the visual system. Its function depends upon its optical clarity so irreversible loss of transparency due to disease or damage results in permanent vision loss or blindness, necessitating corneal transplantation (keratoplasty) in entirety or in part. While keratoplasty is considered as one of the most successful forms of transplantation, lack of availability of donor tissues and rejection are major limiting factors. Advances in knowledge of biomaterials and stem cell biology have paved the way for tissue engineering of various organs including cornea. An ideal biomimetic for corneal tissue replacement would be the one which is transparent, provides mechanical support, promotes epithelial resurfacing, corneal innervation, and integrates into the surrounding corneo-scleral tissues and combats infection when challenged. This chapter reviews several of the advances made in development of biomaterials for promoting regeneration of the human cornea, with or without exogenous cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Notes
- 1.
Regenerative Medicine in Corneal Applications.
References
Ahmed TA, Giulivi A, Griffith M et al (2011) Fibrin glues in combination with mesenchymal stem cells to develop a tissue-engineered cartilage substitute. Tissue Eng Part A 17(3–4):323–335
Ahmed TA, Ringuette R, Wallace VA et al (2015) Autologous fibrin glue as an encapsulating scaffold for delivery of retinal progenitor cells. Front Bioeng Biotechnol 2:85
Anseth A (1961) Studies on corneal polysaccharides. III. Topographic and comparative biochemistry. Exp Eye Res 1:106–115
Aucoin L, Griffith CM, Pleizier G et al (2002) Interactions of corneal epithelial cells and surfaces modified with cell adhesion peptide combinations. J Biomater Sci Polym Ed 13:447–462
Avadhanam VS, Liu CS (2015) A brief review of Boston type-1 and osteo-odonto keratoprostheses. Br J Ophthalmol 99(7):878–887
Axelsson I, Heinegard D (1975) Fractionation of proteoglycans from bovine corneal stroma. Biochem J 145:491–500
Behlau I, Mukherjee K, Todani A et al (2011) Biocompatibility and biofilm inhibition of N, N-hexyl, methyl-polyethylenimine bonded to Boston Keratoprosthesis materials. Biomaterials 32(34):8783–8796
Boucher C, Ruiz JC, Thibault M et al (2010) Human corneal epithelial cell response to epidermal growth factor tethered via coiled-coil interactions. Biomaterials 31(27):7021–7031
Bray LJ, George KA, Ainscough SL et al (2011) Human corneal epithelial equivalents constructed on Bombyx mori silk fibroin membranes. Biomaterials 32(22):5086– 5091
Bray LJ, George KA, Hutmacher DW et al (2012) A dual-layer silk fibroin scaffold for reconstructing the human corneal limbus. Biomaterials 33(13):3529–3538
Brown RA, Mudera V (2012) Plastic compaction of a collagen gel. Patent WO2012004564, 12
Brown RA, Wiseman M, Chuo C et al (2005) Ultrarapid engineering of biomimetic materials and tissues: fabrication of nano-and microstructures by plastic compression. Adv Funct Mater 15:1762–1770
Brown KD, Low S, Mariappan I et al (2014) Plasma polymer-coated contact lenses for the culture and transfer of corneal epithelial cells in the treatment of limbal stem cell deficiency. Tissue Eng Part A 20(3–4):646–655
Burillon C, Huot L, Justin V et al (2011) Cultured Autologous Oral Mucosal Epithelial Cell-Sheet (CAOMECS) transplantation for the treatment of corneal limbal epithelial stem cell deficiency. Invest Ophthalmol Vis Sci 53(3):1325–1331
Buttafoco L, Kolkman NG, Engbers-Buijtenhuijs P et al (2006) Electrospinning of collagen and elastin for tissue engineering applications. Biomaterials 27(5):724–734
Buznyk O, Pasyechnikova N, Islam MM et al (2015) Bioengineered corneas grafted as alternatives to human donor corneas in three high risk patients. Clin Transl Sci. doi:10.1111/cts.12293
Casper CL, Yang W, Farach-Carson MC et al (2007) Coating electrospun collagen and gelatin fibers with perlecan domain I for increased growth factor binding. Biomacromolecules 8(4):1116–1123
Chae JJ, Ambrose WM, Espinoza FA et al (2015) Regeneration of corneal epithelium utilizing a collagen vitrigel membrane in rabbit models for corneal stromal wound and limbal stem cell deficiency. Acta Ophthalmol 93(1):e57–e66
Chang HY, Luo ZK, Chodosh J et al (2015) Primary implantation of type I Boston keratoprosthesis in nonautoimmune corneal diseases. Cornea 34(3):264–270
Chirila T, Barnard Z, Zainuddin et al (2008) Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders. Tissue Eng A 14:1203–1211
Choi JS, Williams JK, Greven M et al (2010) Bioengineering endothelialized neo-corneas using donor-derived corneal endothelial cells and decellularized corneal stroma. Biomaterials 31:6738–6745
Clinic trial successful for China’s artificial cornea. http://english.cri.cn/7146/2013/10/10/2702s791618.htm
ClinicalTrials.gov. Allogeneic Tissue Engineering (Nanostructured Artificial Human Cornea) in Patients With Corneal Trophic Ulcers in Advanced Stages, Refractory to Conventional Ophthalmic) Treatment. Available from: https://clinicaltrials.gov/ct2/show/NCT01765244
Cosar CB, Sridhar MS, Cohen EJ et al (2002) Indications for penetrating keratoplasty and associated procedures, 1996–2000. Cornea 21:148–151
Coster DJ, Williams KA (2005) The impact of corneal allograft rejection on the long-term outcome of corneal transplantation. Am J Ophthalmol 140(6):1112–1122
Dada T, Sharma N, Vajpayee RB (1999) Indications for pediatric keratoplasty in India. Cornea 18:296–298
Daoud YJ, Smith R, Smith T et al (2011) The intraoperative impression and postoperative outcomes of gamma-irradiated corneas in corneal and glaucoma patch surgery. Cornea 30(12):1387–1391
De Roth A (1940) Plastic repair of conjunctival defects with fetal membrane. Arch Ophthalmol 23:522–525
Deshpande P, Notara M, Bullett N et al (2009) Development of a surface-modified contact lens for the transfer of cultured limbal epithelial cells to the cornea for ocular surface diseases. Tissue Eng Part A 15(10):2889–2902
Doillon CJ, Watsky MA, Hakim M et al (2003) A collagen-based scaffold for a tissue engineered human cornea: physical and physiological properties. Int J Artif Organs 26(8):764–773
Dravida S, Gaddipati S, Griffith M et al (2008) A biomimetic scaffold for culturing limbal stem cells: a promising alternative for clinical transplantation. J Tissue Eng Regen Med 2(5):263–271
Dua HS, Gomes JAP, King AJ (2004) The amniotic membrane in ophthalmology. Surv Ophthalmol 49:51–77
Duffy P, Wolf J, Collins G et al (1974) Letter: possible person-to-person transmission of Creutzfeldt-Jakob disease. N Engl J Med 290:692–693
Edwards M, Clover GM, Brookes N et al (2002) Indications for corneal transplantation in New Zealand: 1991–1999. Cornea 21:152–155
Evans MD, Xie RZ, Fabbri M et al (2000) Epithelialization of a synthetic polymer in the feline cornea: a preliminary study. Invest Ophthalmol Vis Sci 41(7):1674–1680
Fagerholm P, Lagali NS, Carlsson DJ et al (2009) Corneal regeneration following implantation of a biomimetic tissue-engineered substitute. Clin Transl Sci 2:162–164
Fagerholm P, Lagali NS, Merrett K et al (2010) A biosynthetic alternative to human donor tissue for inducing corneal regeneration: 24-month follow-up of a phase 1 clinical study. Science Translat Med 2:46ra61
Fagerholm P, Lagali NS, Ong JA et al (2014) Stable corneal regeneration four years after implantation of a cell-free recombinant human collagen scaffold. Biomaterials 35(8):2420–2427
Gaum L, Reynolds I, Jones MN et al (2012) Tissue and corneal donation and transplantation in the UK. Br J Anaesth 108(Suppl 1):i43–i47
Gil ES, Mandal BB, Park SH et al (2010) Helicoidal multi-lamellar features of RGD-functionalized silk biomaterials for cornealtissue engineering. Biomaterials 31(34):8953–8963
Gomaa A, Comyn O, Liu C (2010) Keratoprostheses in clinical practice – a review. Clin Exp Ophthalmol 38:211–224
Gomes JAP, Romano A, Santos MS et al (2005) Amniotic membrane use in ophthalmology. Curr Opin Ophthalmol 16:233–240
Gonzalez-Andrades M, De La Cruz Cardona J, Ionescu AM et al (2011) Generation of bioengineered corneas with decellularized xenografts and human keratocytes. Invest Ophthalmol Vis Sci 52:215–222
González-Andrades M, Carriel V, Rivera-Izquierdo M et al (2015) Effects of detergent-based protocols on decellularization of corneas with sclerocorneal limbus. Evaluation of regional differences. Transl Vis Sci Technol 4(2):13
Gouveia RM, Castelletto V, Alcock SG et al (2013) Bioactive films produced from self-assembling peptide amphiphiles as versatile substrates for tuning cell adhesion and tissue architecture in serum-free conditions. J Mater Chem B 1:6157–6169
Griffith M, Hakim M, Shimmura S et al (2002) Artificial human corneas: scaffolds for transplantation and host regeneration. Cornea 21(Suppl 2):S1–S8
Griffith M, Jackson WB, Lagali N et al (2009) Artificial corneas: a regenerative medicine approach. Eye (Lond) 23(10):1985–1989
Grolik M, Szczubiałka K, Wowra B et al (2012) Hydrogel membranes based on genipin-cross-linked chitosan blends for corneal epithelium tissue engineering. J Mater Sci Mater Med 23(8):1991–2000
Gupta V, Bambery P, Radotra BD et al (2001) Vogt-Koyanagi-Harada syndrome following injury-induced progressive vitiligo. Indian J Ophthalmol 49:53–55
Hackett JM, Lagali N, Merrett K et al (2011) Biosynthetic corneal implants for replacement of pathologic corneal tissue: performance in a controlled rabbit alkali burn model. Invest Ophthalmol Vis Sci 52(2):651–657
Haldar J, An D, Alvarez de Cienfuegos L et al (2006) Polymeric coatings that inactivate both influenza virus and pathogenic bacteria. Proc Natl Acad Sci U S A 103(47):17667–17671
Hamada R, Giraud JP, Graf B et al (1972) Analytical and statistical study of the lamellae, keratocytes and collagen fibrils of the central region of the normal human cornea. (Light and electron microscopy). Archives D’ophtalmologie Et Revue Générale D’ophtalmologie 32:563–570
Han B, Schwab IR, Madsen TK et al (2002) A fibrin-based bioengineered ocular surface with human corneal epithelial stem cells. Cornea 21:505–510
Hao Y, Ma DH, Hwang DG et al (2000) Identification of antiangiogenic and antiinflammatory proteins in human amniotic membrane. Cornea 19:348–352
Hashimoto Y, Funamoto S, Sasaki S et al (2010) Preparation and characterization of decellularized cornea using high-hydrostatic pressurization for corneal tissue engineering. Biomaterials 31:3941–3948
Hedblom EE (1961) The role of polysaccharides in corneal swelling. Exp Eye Res 1:81–91
Higa K, Shimmura S, Kato N et al (2007) Proliferation and differentiation of transplantable rabbit epithelial sheets engineered with or without an amniotic membrane carrier. Invest Ophthalmol Vis Sci 48:597–604
Hogan MJ, Alvarado JA, Weddell JE (1971) Histology of the human eye. An atlas and textbook. Saunders Company, Philadelphia/London/Toronto
Houff SA, Burton RC, Wilson RW et al (1979) Human-to-human transmission of rabies virus by corneal transplant. N Engl J Med 300:603–604
Jacob JT, Rochefort JR, Bi J et al (2005) Corneal epithelial cell growth over tethered-protein/peptide surface-modified hydrogels. J Biomed Mater Res Part B Appl Biomater 72:198–205
Karamichos D, Rich CB, Hutcheon AE et al (2011) Self-assembled matrix by umbilical cord stem cells. J Funct Biomater 2(3):213–229
Karkhaneh A, Mirzadeh H, Ghaffariyeh A et al (2011) Novel materials to enhance corneal epithelial cell migration on keratoprosthesis. Br J Ophthalmol 95(3):405–409
Kenyon KR (1983) Morphology and pathologic responses of the cornea to disease. Smolin and Thoft’s the cornea: scientific foundations and clinical practice. Little, Brown & Co, Boston
Khan B, Dudenhoefer EJ, Dohlman CH (2001) Keratoprosthesis: an update. Curr Opin Ophthalmol 12:282–287
Kim EY, Tripathy N, Park JY et al (2015) Silk fibroin film as an efficient carrier for corneal endothelial cells regeneration. Macromol Res 23(2):189–195
Kinoshita JH, Kador P, Catiles M (1981) Aldose reductase in diabetic cataracts. JAMA: J Am Med Assoc 246:257–261
Klenkler BJ, Griffith M, Becerril C et al (2005) EGF-grafted PDMS surfaces in artificial cornea applications. Biomaterials 26:7286–7296
Klibanov AM (2007) Permanently microbicidal materials coatings. J Mater Chem 17:2479–2482
Klyce SD, Beuerman RW (1988) Structure and function of the cornea. In: Kaufman HE, Barron BA, McDonald MB, Waltman SR (eds) The cornea. Churchill Livingstone, New York
Kobayashi H, Ikada Y (1991) Corneal cell adhesion and proliferation on hydrogel sheets bound with cell-adhesive proteins. Curr Eye Res 10:899–908
Koizumi NJ, Inatomi TJ, Sotozono CJ et al (2000) Growth factor mRNA and protein in preserved human amniotic membrane. Curr Eye Res 20:173–177
Kuhl PR, Griffith-Cima LG (1996) Tethered epidermal growth factor as a paradigm for growth factor-induced stimulation from the solid phase. Nat Med 2:1022–1027
Kumar P, Satyam A, Fan X et al (2015) Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies. Sci Rep 5:8729
Kureshi AK, Drake RA, Daniels JT (2014) Challenges in the development of a reference standard and potency assay for the clinical production of RAFT tissue equivalents for the cornea. Regen Med 9(2):167–177
Lawrence BD, Marchant JK, Pindrus MA et al (2009) Silk film biomaterials for cornea tissue engineering. Biomaterials 30(7):1299–1308
Lee SH, Chun YS, Kim JC (2011) The study of characteristics of acellular porcine cornea using freezing-thawing-centrifugation. J Korean Ophthalmol Soc 52:86
Levis HJ, Brown RA, Daniels JT (2010) Plastic compressed collagen as a biomimetic substrate for human limbal epithelial cell culture. Biomaterials 31(30):7726–7737
Levis HJ, Kureshi AK, Massie I et al (2015) Tissue engineering the cornea: the evolution of RAFT. J Funct Biomater 6(1):50–65
Lewis K, Klibanov AM (2005) Surpassing nature: rational design of sterile-surface materials. Trends Biotechnol 23(7):343–348
Li F, Carlsson D, Lohmann C et al (2003) Cellular and nerve regeneration within a biosynthetic extracellular matrix for corneal transplantation. Proc Natl Acad Sci U S A 100:15346–15351
Lin J, Tiller JC, Lee SB et al (2002) Insights into bactericidal action of surface-attached poly(vinyl-N-hexypyridinium) chains. Biotechnol Lett 24:801–805
Liu W, Deng C, Mclaughlin CR et al (2009) Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes. Biomaterials 30:1551–1559
Madden PW, Lai JN, George KA et al (2011) Human corneal endothelial cell growth on a silk fibroin membrane. Biomaterials 32(17):4076–4084
Madhavan HN, Malathi J, Joseph RP et al (2004) A study on the growth of continuous culture cell lines embedded in Mebiol Gel. Curr Sci 87:1275–1277
Massie I, Dale SB, Daniels JT (2014) Limbal fibroblasts maintain normal phenotype in 3D RAFT tissue equivalents suggesting potential for safe clinical use in treatment of ocular surface failure. Tissue Eng Part C Methods [Epub ahead of print]
Matthews JA, Wnek GE, Simpson DG et al (2002) Electrospinning of collagen nanofibers. Biomacromolecules 3(2):232–238
McIntosh Ambrose W, Salahuddin A, So S et al (2009) Collagen Vitrigel membranes for the in vitro reconstruction of separate corneal epithelial, stromal, and endothelial cell layers. J Biomed Mater Res Part B Appl Biomater 90:818–831
Meek KM, Leonard DW (1993) Ultrastructure of the corneal stroma: a comparative study. Biophys J 64:273–280
Merrett K, Griffith CM, Deslandes Y et al (2001) Adhesion of corneal epithelial cells to cell adhesion peptide modified pHEMA surfaces. J Biomater Sci Polym Ed 12:647–671
Milovic NM, Wang J, Lewis K (2005) Immobilized N-alkylated polyethylenimine avidly kills bacteria by rupturing cell membranes with no resistance developed. Biotechnol Bioeng 90:715–722
Minami Y, Sugihara H, Oono S (1993) Reconstruction of cornea in three-dimensional collagen gel matrix culture. Invest Ophthalmol Vis Sci 34(7):2316–2324
Miyashita H, Shimmura S, Kobayashi H et al (2006) Collagen-immobilized poly(vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium. J Biomed Mater Res Part B Appl Biomater 76:56–63
Muraine MC, Collet A, Brasseur G (2002) Deep lamellar keratoplasty combined with cataract surgery. Arch Ophthalmol 120:812–815
Myung D, Koh W, Bakri A et al (2007) Design and fabrication of an artificial cornea based on a photolithographically patterned hydrogel construct. Biomed Microdevices 9:911–922
Myung D, Duhamel P-E, Cochran JR, Noolandi J et al (2008) Development of hydrogel-based keratoprostheses: a materials perspective. Biotechnol Prog 24:735–741
Myung D, Farooqui N, Zheng LL et al (2009) Bioactive interpenetrating polymer network hydrogels that support corneal epithelial wound healing. J Biomed Mater Res Part A 90:70–81
Nakamura T, Takeda K, Inatomi T et al (2011) Long-term results of autologous cultivated oral mucosal epithelial transplantation in the scar phase of severe ocular surface disorders. Br J Ophthalmol 95:942–946
Neel EAA, Cheema U, Knowles JC et al (2006) Use of multiple unconfined compression for control of collagen gel scaffold density and mechanical properties. Soft Matter 2:986–992
Nishida K, Yamato M, Hayashida Y et al (2004a) Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface. Transplantation 77:379–385
Nishida K, Yamato M, Hayashida Y et al (2004b) Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. N Engl J Med 351:1187–1196
Ponce Marquez S, Martinez VS, McInthosh Ambrose W et al (2009) Decellularization of bovine corneas for tissue engineering applications. Acta Biomater 5:1839–1847
Poole CA, Brookes NH, Clover (1996) Confocal imaging of the keratocyte network in porcine cornea using the fixable vital dye 5-chloromethylfluorescein diacetate. Curr Eye Res 15:165–174
Proulx S, d’Arc Uwamaliya J, Carrier P et al (2010) Reconstruction of a human cornea by the self-assembly approach of tissue engineering using the three native cell types. Mol Vis 16:2192–2201
Rafat M, Li F, Fagerholm P et al (2008) Peg-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering. Biomaterials 29:3960–3972
Rafat MA, Hackett JM, Fagerholm P et al (2010) Artificial cornea. In: Dartt DA, Besharse J, Dana R (eds) Encyclopedia of the eye. Academic, Boston
Rama P, Bonni S, Lambiase et al (2001) Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency. Transplantation 72:1478–1485
Ramachandran C, Basu S, Sangwan VS et al (2014) Concise review: the coming of age of stem cell treatment for corneal surface damage. Stem Cells Transl Med 3(10):1160–1168
Reichl S, Müller-Goymann CC (2003) The use of a porcine organotypic cornea construct for permeation studies from formulations containing befunolol hydrochloride. Int J Pharm 250(1):191–201
Sangwan VS, Gopinathan U, Garg P et al (2010) Eye banking in India: a road ahead. JIMSA 23:197–200
Sangwan VS, Jain R, Basu S et al (2014) Transforming ocular surface stem cell research into successful clinical practice. Indian J Ophthalmol 62:29–40
Satake Y, Higa K, Tsubota K et al (2011) Long-term outcome of cultivated oral mucosal epithelial sheet transplantation in treatment of total limbal stem cell deficiency. Ophthalmology 118:1524–1530
Secker GA, Daniels JT (2009) Limbal epithelial stem cells of the cornea. In: StemBook (ed) The stem cell research community, StemBook, Harvard Stem Cell Institute, Cambridge, MA
Sheth R, Neale MH, Shortt AJ et al (2014) Culture and characterization of oral mucosal epithelial cells on a fibrin gel for ocular surface reconstruction. Curr Eye Res 7:1–11
Shibasaki Y, Hirohara S, Terada K et al (2011) Collagen-like polypeptide poly(Pro-Hyp-Gly) conjugated with Gly-Arg-Gly-Asp-Ser and Pro-His-Ser-Arg-Asn peptides enhances cell adhesion, migration, and stratification. Biopolymers 96(3):302–315
Shimmura S, Doillon CJ, Griffith M et al (2003) Collagen-poly(N-isopropylacrylamide)-based membranes for corneal stroma scaffolds. Cornea 22:S81–S88
Shortt AJ, Secker GA, Lomas RJ et al (2009) The effect of amniotic membrane preparation method on its ability to serve as a substrate for the ex-vivo expansion of limbal epithelial cells. Biomaterials 30:1056–1065
Sitalakshmi G, Sudha B, Madhavan HN et al (2009) Ex vivo cultivation of corneal limbal epithelial cells in a thermoreversible polymer (Mebiol Gel) and their transplantation in rabbits: an animal model. Tissue Eng Part A 15(2):407–415
Sudha B, Madhavan HN, Sitalakshmi G et al (2006) Cultivation of human corneal limbal stem cells in Mebiol gel-A thermo-reversible gelation polymer. Indian J Med Res 124:655–664
Sweeney DF, Xie RZ, Evans MDM et al (2003) A comparison of biological coatings for the promotion of corneal epithelialization of synthetic surface in vivo. Invest Ophthalmol Vis Sci 44:3301–3309
Takezawa T, Ozaki K, Nitani A et al (2004) Collagen vitrigel: a novel scaffold that can facilitate a three-dimensional culture for reconstructing organoids. Cell Transplant 13:463
Takezawa T, McIntosh-Ambrose W, Elisseeff JH (2008) A novel culture model of rabbit corneal epithelium utilizing a handy scaffold of collagen vitrigel membrane and its cryopreservation. Altern Anim Test Exp 13(Suppl):176
Takezawa T, Nishikawa K, Wang PC (2011) Development of a human corneal epithelium model utilizing a collagen vitrigel membrane and the changes of its barrier function induced by exposing eye irritant chemicals. Toxicol In Vitro 25:1237–1241
Tan XW, Goh TW, Saraswathi P et al (2014) Effectiveness of antimicrobial peptide immobilization for preventing perioperative cornea implant-associated bacterial infection. Antimicrob Agents Chemother 58:5229–5238
Tidu A, Ghoubay-Benallaoua D, Lynch B et al (2015) Development of human corneal epithelium on organized fibrillated transparent collagen matrices synthesized at high concentration. Acta Biomater 22:50–58
Tosi GM, Massaro-Giordano M, Caporossi A et al (2005) Amniotic membrane transplantation in ocular surface disorders. J Cell Physiol 202:849–851
Trinkaus-Randall V (2000) Cornea: biological responses. In: Lanza R, Langer R, Chick E (eds) Principles of tissue engineering, 2nd edn. Academic, London, pp 471–491
Tseng SC, Li DQ, MA X (1999) Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix. J Cell Physiol 179:325–335
Uchino Y, Shimmura S, Miyashita H et al (2007) Amniotic membrane immobilized poly(vinyl alcohol) hybrid polymer as an artificial cornea scaffold that supports a stratified and differentiated corneal epithelium. J Biomed Mater Res Part B Appl Biomater 81:201–206
Uzunalli G, Soran Z, Erkal TS et al (2014) Bioactive self-assembled peptide nanofibers for corneal stroma regeneration. Acta Biomater 10:1156–1166
Vemuganti GK, Fatima A, Madhira SL (2009) Limbal stem cells: application in ocular biomedicine. Int Rev Cell Mol Biol 275:133–181
Vrana NE, Builles N, Justin V et al (2008) Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures. Invest Ophthalmol Vis Sci 49:5325
Wallace C, Jacob JT, Stoltz A et al (2005) Corneal epithelial adhesion strength to tethered-protein/peptide modified hydrogel surfaces. J Biomed Mater Res Part A 72:19–24
Wang L, Jeong KJ, Chiang HH et al (2011) Hydroxyapatite for keratoprosthesis biointegration. Invest Ophthalmol Vis Sci 52:7392–7399
Wang HY, Wei RH, Zhao SZ (2013) Evaluation of corneal cell growth on tissue engineering materials as artificial cornea scaffolds. Int J Ophthalmol 6:873–878
Werner A, Braun M, Reichl S et al (2008) Establishing and functional testing of a canine corneal construct. Vet Ophthalmol 11:280–289
Whitcher JP, Srinivasan M, Upadhyay MP (2001) Corneal blindness: a global perspective. Bull World Health Organ 79:214–221
Wilson SE, Hong JW (2000) Bowman’s layer structure and function: critical or dispensable to corneal function? A hypothesis. Cornea 19:417–420
Wilson SL, Sidney LE, Dunphy SE et al (2013) Keeping an eye on decellularized corneas: a review of methods, characterization and applications. J Funct Biomater 4:114–161
Wray LS, Orwin EJ (2009) Recreating the microenvironment of the native cornea for tissue engineering applications. Tissue Eng Part A 15(7):1463–1472
Yamato M, Utsumi M, Kushida A et al (2001) Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature. Tissue Eng 7:473–480
Zhang S, Holmes T, Lockshin C et al (1993) Spontaneous assembly of a self-complementary oligopeptide to form a stable macroscopic membrane. Proc Natl Acad Sci U S A 90:3334–3338
Zhang M-C, Liu X, Jin Y et al (2015) Lamellar keratoplasty treatment of fungal corneal ulcers with acellular porcine corneal stroma. Am J Transplant 15:1068–1075
Zhong S, Teo WE, Zhu X et al (2006) An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture. J Biomed Mater Res A 79(3):456–463
Zieske JD, Mason VS, Wasson ME et al (1994) Basement membrane assembly and differentiation of cultured corneal cells: importance of culture environment and endothelial cell interaction. ExpCell Res 214(2):621–633
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Polisetti, N., Vemuganti, G.K., Griffith, M. (2016). Biomaterials-Enabled Regenerative Medicine in Corneal Applications. In: Steinhoff, G. (eds) Regenerative Medicine - from Protocol to Patient. Springer, Cham. https://doi.org/10.1007/978-3-319-28274-9_5
Download citation
DOI: https://doi.org/10.1007/978-3-319-28274-9_5
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-28272-5
Online ISBN: 978-3-319-28274-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)