Skip to main content
SpringerLink
Log in

Population Genetic Structure of Two Threatened Dragonfly Species (Odonata: Anisoptera) as Revealed by RAPD Analysis

  • Conference paper
  • First Online:
Relict Species

  • 1535 Accesses

Abstract

The riverine odonates Macromia splendens and Oxygastra curtisii were included in the European Habitats directive as taxa of special concern. Nevertheless, there is almost no genetic information about them. We assessed the genetic diversity and population structuring among several Northwest Iberian locations where these species occur. For this, we examined the genetic pattern revealed by RAPD markers in four locations of M. splendens and five locations of O. curtisii. The former showed strong population structuring, whereas gene flow between different river systems may be the reason for the lower structuring inferred for O. curtisii. Based on these results, we support the need of special management for M. splendens in Northwest Iberia.

The online version of the Erratum chapter can be found at http://dx.doi.org/10.1007/978-3-540-92160-8_27

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  • Aagaard JE, Krutovskii KV, Strauss SH (1998) RAPDs and allozymes exhibit similar levels of diversity and differentiation among populations and races of Douglas-fir. Heredity 81:69–78

    Article  CAS  Google Scholar 

  • Abbott JK, Bensch S, Gosden TP, Svensson EI (2008) Patterns of differentiation in a colour polymorphism and in neutral markers reveal rapid genetic changes in natural damselfly populations. Mol Ecol 17(6):1597-1604. doi:10.1111/j.1365-294X.2007.03641.x

    Article  CAS  PubMed  Google Scholar 

  • Andrés JA, Sánchez-Guillén RA, Cordero Rivera A (2000) Molecular evidence for selection of female color polymorphis in the damselfly Ischnura graellsii. Evolution 54:2156–2161

    PubMed  Google Scholar 

  • Andrés JA, Sánchez-Guillén RA, Cordero Rivera A (2002) Evolution of female colour polymorphism in damselflies: testing the hypotheses. Anim Behav 63:677–685

    Article  Google Scholar 

  • Azpilicueta Amorín M, Rey Rañó C, Docampo Barrueco F, Rey Muñiz XC, Cordero Rivera A (2007) A preliminary study of biodiversity hotspots for odonates in Galicia, NW Spain. Odonatologica 36:1–12

    Google Scholar 

  • Boudot J-P (2006) Macromia splendens. In IUCN 2007. 2007 IUCN Red List of Threatened Species. Available at http://www.iucnredlist.org. Cited 6 Feb 2008

  • Boudot J-P, Riservato E, Hardersen S (2006) Oxygastra curtisii. In IUCN 2007. 2007 IUCN Red List of Threatened Species. Available at http://www.iucnredlist.org. Cited 6 Feb 2008

  • Cordero Rivera A (2000) Distribution, habitat requirements and conservation of Macromia splendens Pictet (Odonata: Corduliidae) in Galicia (NW Spain). Int J Odonatol 3:73–83

    Google Scholar 

  • Cordero Rivera A (ed) (2006) Forests and dragonflies. Pensoft publishers, Sofia, Moscow

    Google Scholar 

  • Cordero Rivera A, Utzeri C, Santolamazza Carbone S (1999) Emergence and adult behaviour of Macromia splendens (Pictet) in Galicia, Northwestern Spain (Anisoptera. Corduliidae). Odonatologica 28:333–342

    Google Scholar 

  • d’Aguilar JD, Dommanget JL (1998) Guide des Libellules d’Europe et d’Afrique du Nord. Delachaux et Niestlé, Lausanne

    Google Scholar 

  • De Block M, Geenen S, Jordaens K, Backeljau T, Stoks T (2005) Spatiotemporal allozyme variation in the damselfly, Lestes viridis (Odonata: Zygoptera): gene flow among permanent and temporary ponds. Genetica 124:137–144

    Article  PubMed  Google Scholar 

  • Dommanget JL, Grand D (1996) Macromia splendens (Pictet, 1843). In: Van Helsdingen PJ, Willemse L, Speight MCD (eds) Background information on invertebrates of the habitats directive and the bern convention. Part II - Mantodea, odonata, orthoptera and arachnida. Council of Europe, Strassbourg, pp 315–323

    Google Scholar 

  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620

    Article  CAS  PubMed  Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  PubMed  Google Scholar 

  • Falush D, Stephens M, Pritchard JK (2003) Inference of population structure: extensions to linked loci and correlated frequencies. Genetics 164:1567–1587

    CAS  PubMed  Google Scholar 

  • Foote AL, Rice CL (2005) Odonates as biological indicators of grazing effects on Canadian prairie wetlands. Ecol Entomol 30:273–283

    Article  Google Scholar 

  • Gadeberg RME, Boomsma JJ (1997) Genetic population structure of the large blue butterfly Maculinea alcon in Denmark. J Insect Conserv 1:99–111

    Article  Google Scholar 

  • Geenen S, Jordaens K, De Block M, Stoks R, De Bruyn L (2000) Genetic differentiation and dispersal among populations of the damselfly Lestes viridis (Odonata). J North Am Benthol Soc 19:321–328

    Article  Google Scholar 

  • Giere S, Hadrys H (2006) Polymorphic microsatellite loci to study population dynamics in a dragonfly, the libellulid Trithemis arteriosa (Burmeister, 1839). Mol Ecol Notes 6:933–935

    Article  CAS  Google Scholar 

  • Hadrys H, Schroth W, Schierwater B, Streit B, Fincke OM (2005) Tree hole odonates as environmental monitors: non-invasive isolation of polymorphic microsatellites from the neotropical damselfly Megaloprepus caerulatus. Conserv Genet 6:481–483

    Article  Google Scholar 

  • Hadrys H, Clausnitzer V, Groenefeld LF (2006) The present role and future promise of conservation genetics for forest Odonates. In: Cordero Rivera A (ed) Forest and dragonflies. 4th WDA International symposium of odonatology, pontevedra (Spain), July 2005. Pensoft publishers, Sofia, Moscow, pp 279–299

    Google Scholar 

  • Jacquemin G, Boudot JP (1999) Les libellules (Odonates) du Maroc. Sociêté Française d’Odonatologie, Paris

    Google Scholar 

  • Jonsson M, Johannesen J, Seitz A (2003) Comparative genetic structure of the threatened tenebrionid beetle Oplocephala haemorrhoidalis and its common relative Bolitophagus reticulatus. J Insect Conserv 7:111–124

    Article  Google Scholar 

  • Keat S, Thompson DJ, Kemp SJ, Watts PC (2005) Ten microsatellite loci for the red-eyed damselfly Erythromma viridulum (Charpentier). Mol Ecol Notes 5:788–790

    Article  CAS  Google Scholar 

  • Lai B-CG, Pullin AS (2004) Phylogeography, genetic diversity and conservation of the large copper butterfly Lycaena dispar in Europe. J Insect Conserv 8:27–36

    Article  Google Scholar 

  • Legge J, Roush R, Desalle R, Vogler A, May B (1996) Genetic criteria for establishing evolutionarily significant units in Cryan’s buckmoth. Conserv Biol 10:85–98

    Article  Google Scholar 

  • Lieftinck MA (1965) Macromia splendens (Pictet, 1843) in Europe with notes on its habits, larva and distribution (Odonata). Tijdschr Entomol 108:41–59

    Google Scholar 

  • Lorenzo Carballa O, Giere S, Cordero A, Hadrys H (2007) Isolation and characterization of microsatellite loci to study parthenogenesis in the citrine forktail, Ischnura hastata (Odonata: Coenagrionidae). Mol Ecol Notes 7:839–841

    Article  Google Scholar 

  • Louy D, Habel J, Schmitt T, Meyer M, Assmann T, Müller P (2007) Strongly diverging population genetic patterns of three skipper species: isolation, restricted gene flow and panmixis. Conserv Genet 8:671–681

    Article  Google Scholar 

  • Miller MP (1997) Tools For Population Genetic Analyses (TFPGA) A Windows™ program for the analysis of allozyme and molecular population genetic data. http://www.marksgeneticsoftware.net/tfpga.htm. Cited 20 May 2007

  • Ocharan FJ, Ferreras Romero M, Ocharan R, Cordero Rivera A (2006) Macromia splendens (Pictet, 1843). In: Verdú JR, Galante E (eds) Libro Rojo de los Invertebrados de España. Dirección General de Conservación de la Naturaleza, Madrid, pp 258–259

    Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    CAS  PubMed  Google Scholar 

  • Rasplus JY, Garnier S, Meusnier S, Piry S, Mondor G, Audiot P, Cornuet JM (2001) Setting conservation priorities: the case study of Carabus solieri (Col. Carabidae). Genet Select Evol 33:S141-S175

    CAS  Google Scholar 

  • Raymond M, Rousset F (1995) An exact test for population differentiation. Evolution 49:1280–1283

    Article  Google Scholar 

  • Rouquette JR, Thompson DJ (2005) Habitat associations of the endangered damselfly Coenagrion mercuriale, in a water meadow ditch system in Southern England. Biol Conserv 123:225–235

    Article  Google Scholar 

  • Sahlén G, Bernard R, Cordero Rivera A, Ketelaar R, Suhling F (2004) Critical species of Odonata in Europe. Int J Odonatol 7:385–398

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis F (1989) Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Svensson EI, Kristoffersen L, Oskarsson K, Bensch S (2004) Molecular population divergence and sexual selection on morphology in the banded demoiselle (Calopteryx splendens). Heredity 93:423–433

    Article  CAS  PubMed  Google Scholar 

  • Thompson DJ, Watts PC (2006) The structure of the Coenagrion mercuriale populations in the New Forest, Southern England. In: Cordero Rivera A (ed) Forest and Dragonflies. 4th WDA International symposium of odonatology, pontevedra (Spain), July 2005. Pensoft publishers, Sofia, Moscow, pp 239–258

    Google Scholar 

  • Turner CR (2007) Water beetles associated with reservoirs on table mountain, cape town: implications for conservation. J Insect Conserv 11:75–83

    Article  Google Scholar 

  • Volis S, Yakubov B, Shulgina I, Ward D, Mendlinger S (2005) Distinguishing adaptive from nonadaptive genetic differentiation: comparison of QST and FST at two spatial scales. Heredity 95:466–475

    Article  CAS  PubMed  Google Scholar 

  • Watanabe K, Omura T (2007) Relationship between reservoir size and genetic differentiation of the stream caddisfly Stenopsyche marmorata. Biol Conserv 136:203–211

    Article  Google Scholar 

  • Watts PC, Rouquette JR, Saccheri IJ, Kemp SJ, Thompson DJ (2004) Molecular and ecological evidence for small-scale isolation by distance in an endangered damselfly, Coenagrion mercuriale. Mol Ecol 13:2931–2945

    Article  CAS  PubMed  Google Scholar 

  • Watts PC, Kemp SJ, Saccheri IJ, Thompson DJ (2005a) Conservation implications of genetic variation between spatially and temporally distinct colonies of the endangered damselfly Coenagrion mercuriale. Ecol Entomol 30:541–547

    Article  Google Scholar 

  • Watts PC, Thompson DJ, Daguet C, Kemp SJ (2005b) Exuviae as a reliable source of DNA for population-genetic analysis of odonates. Odonatologica 34:183–187

    Google Scholar 

  • Watts PC, Saccheri IJ, Kemp SJ, Thompson DJ (2006) Population structure and the impact of regional and local habitat isolation upon levels of genetic diversity of the endangered damselfly Coenagrion mercuriale (Odonata: Zygoptera). Freshw Biol 51:193–205

    Article  CAS  Google Scholar 

  • Watts PC, Rousset F, Saccheri IJ, Leblois R, Kemp SJ, Thompson DJ (2007) Compatible genetic and ecological estimates of dispersal rates in insect (Coenagrion mercuriale: Odonata: Zygoptera) populations: analysis of “neighbourhood size” using a more precise estimator. Mol Ecol 16:737–751

    Article  PubMed  Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370

    Article  Google Scholar 

  • Wong A, Smith ML, Forbes MR (2003) Differentiation between subpopulations of a polychromatic damselfly with respect to morph frequencies, but not neutral genetic markers. Mol Ecol 12:3505–3513

    Article  CAS  PubMed  Google Scholar 

  • Yeh FC, Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg J Bot 129:157

    Google Scholar 

Download references

Acknowledgements

M. Pimentel and one anonymous referee improved the earlier versions of the manuscript with their comments. R. Sánchez-Guillén kindly helped in the laboratory. This work is part of MAA’s PhD thesis. D. Romero designed Fig. 1. Funding was provided by grants from Spanish Ministry of Science (BOS2001-3642) and Xunta de Galicia (PGIDT01MAM37101PR) to ACR. MV was financed by the Isidro Parga Pondal program and the University of A Coruna.

Author information

Authors and Affiliations

  1. Department of Ecology and Animal Biology, University of Vigo, EUET Forestal, Campus Universitario, E-36006, Pontevedra, Spain

    Mónica A. Amorín & Adolfo C. Rivera

  2. Department of Molecular and Cell Biology, University of La Coruña, Faculty of Sciences, Campus Zapateira, E-15008, La Coruña, Spain

    Marta Vila

Authors
  1. Mónica A. Amorín
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marta Vila
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Adolfo C. Rivera
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adolfo C. Rivera .

Editor information

Editors and Affiliations

  1. Musée National d´Histoire Naturelle Luxembourg, 25, rue Münster, 2160, Luxembourg

    Jan Christian Habel

  2. Institute of Ecology and Environmental Chemistry, Leuphana University Lüneburg, Scharnhorststr. 1, 21335, Lüneburg, Germany

    Thorsten Assmann

Rights and permissions

Reprints and permissions

Copyright information

© 2010 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Amorín, M.A., Vila, M., Rivera, A.C. (2010). Population Genetic Structure of Two Threatened Dragonfly Species (Odonata: Anisoptera) as Revealed by RAPD Analysis. In: Habel, J.C., Assmann, T. (eds) Relict Species. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-92160-8_17

Download citation

Publish with us

Policies and ethics

Search

Navigation