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Ovarian Tissue Cryopreservation: Slow Freezing

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Gonadal Tissue Cryopreservation in Fertility Preservation

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Abstract

The needs for fertility preservation (FP) have substantially increased due to improvements in early diagnosis and survival of many malignancies experienced during the last decades. Different FP options can be offered nowadays, but ovarian tissue cryopreservation and retransplantation is the preferred option in prepubertal girls or when there is an urgent need to start the treatment (no time for ovarian stimulation). In this chapter, the basic principles of cryopreservation will be reviewed. A comprehensive and clinical practice-oriented description of the retrieval, preparation, and slow-freezing procedure of the tissue will be provided, together with a summary of the cryopreservation protocols that have been proven to be effective by obtaining live births.

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Authors and Affiliations

  1. Reproductive Medicine Research Group, IIS La Fe. La Fe University Hospital, Valencia, Spain

    Sonia Herraiz, Cesar Diaz-Garcia & Antonio Pellicer

  2. IVI Foundation, Valencia, Spain

    Sonia Herraiz & Antonio Pellicer

  3. Department of Pediatrics, Obstetrics and Gynecology, University of Valencia, Valencia, Spain

    Sonia Herraiz, Cesar Diaz-Garcia & Antonio Pellicer

Authors
  1. Sonia Herraiz
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  2. Cesar Diaz-Garcia
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  3. Antonio Pellicer
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Correspondence to Cesar Diaz-Garcia .

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Editors and Affiliations

  1. Department of Obstetrics and Gynecology, St. Marianna University School of Medici, Kawasaki, Kanagawa, Japan

    Nao Suzuki

  2. Societe de Recherche pour l'Inferti, Brussels, Belgium

    Jacques Donnez

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Herraiz, S., Diaz-Garcia, C., Pellicer, A. (2016). Ovarian Tissue Cryopreservation: Slow Freezing. In: Suzuki, N., Donnez, J. (eds) Gonadal Tissue Cryopreservation in Fertility Preservation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55963-4_4

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