Abstract
Atomic force microscopy (AFM) operates on a very different principle than other forms of microscopy, such as optical microscopy or electron microscopy. The key component of an AFM is a cantilever that bends in response to forces that it experiences as it touches another surface. Forces as small as a few picoNewtons can be detected and probed with AFM. AFM has become very useful in biological sciences because it can be used on living cells that are immersed in water. AFM is particularly useful when the cantilever is modified with chemical groups (e.g. amine or carboxylic groups), small beads (e.g. glass or latex), or even a bacterium. This chapter describes how AFM can be used to measure forces and bonds between a bacterium and another surface. This paper also provides an example of the use of AFM on Staphylococcus aureus, a Gram-positive bacterium that is often associated with biofilms in humans.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Albrecht TR, Akamine S, Carver TE, Quate CF (1990) Microfabrication of cantilever styli for the atomic force microscope. J Vac Sci Technol A 8:3386–3396
Bell GI (1978) Models for specific adhesion of cells to cells. Science 200:618–627
Bemis JE, Akhremitchev BB, Walker GC (1999) Single polymer chain elongation by atomic force microscopy. Langmuir 15:2799–2805
Benoit M, Gabriel D, Gerisch G, Gaub HE (2000) Discrete interactions in cell adhesion measured by single-molecule force spectroscopy. Nat Cell Biol 2:313–317
Binnig G, Quate CF, Gerber C (1986) Atomic force microscope. Phys Rev Lett 56:930–933
Butt HJ, Kappl M, Mueller H, Raiteri R, Meyer W, Ruhe J (1999) Steric forces measured with the atomic force microscope at various temperatures. Langmuir 15:2559–2565
Camesano TA, Logan BE (2000) Probing bacterial electrosteric interactions using atomic force microscopy. Environ. Sci. Technol. 34:3354–3362
Cappella B, Dietler G (1999) Force-distance curves by atomic force microscopy. Surf Sci Rep 34:1–44
Carrion-Vazquez M, Oberhauser AF, Fowler SB, Marszalek PE, Broedel SE, Clarke J, Fernandez JM (1999) Mechanical and chemical unfolding of a single protein: a comparison. Proc Natl Acad Sci USA 96:3694–3699
Cleveland JP, Manne S, Bocek D, Hansma PK (1993) A nondestructive method for determining the spring constant of cantilevers for scanning force microscopy. Rev Sci Instrum 64:403–405
Craig VSJ, Neto C (2001) In situ calibration of colloid probe cantilevers in force microscopy: hydrodynamic drag on a sphere approaching a wall. Langmuir 17:6018–6022
Ducker WA, Senden TJ, Pashley RM (1991) Direct measurement of colloidal forces using an atomic force microscope. Nature 353:239–241
Ducker WA, Senden TJ, Pashley RM (1992) Measurements of forces in liquids using a force microscope. Langmuir 8:1831–1836
Dugdale TM, Dagastine R, Chiovitti A, Mulvaney P, Wetherbee R (2005) Single adhesive nanofibers from a live diatom have the signature fingerprint of modular proteins. Biophys J 89:4252–4260
Dugdale TM, Dagastine R, Chiovitti A, Wetherbee R (2006) Diatom adhesive mucilage contains distinct supramolecular assemblies of a single modular protein. Biophys J 90:2987–2993
Elimelech M, Gregory J, Jia X, Williams R (1995) Particle deposition & aggregation: measurement, modeling, and simulation. Butterworth-Heinemann, Oxford
Evans E (2001) Probing the relation between force – lifetime – and chemistry in single molecular bonds. Annu Rev Biophys Biomol Struct 30:105–128
Flory PJ (1989) Statistical mechanics of chain molecules. Hanser Publisher, Munich, Germany
Foster TJ, Höök M (1998) Surface protein adhesins of Staphylococcus aureus. Trends Microbiol 6:484–488
Fröman G, Switalski LM, Speziale P, Höök M (1987) Isolation and characterization of a fibronectin receptor from Staphylococcus aureus. J Biol Chem 262:6564–6571
Greene C, McDevitt D, Francois P, Vaudaux PE, Lew DP, Foster TJ (1995) Adhesion properties of mutants of Staphylococcus aureus defective in fibronectin-binding proteins and studies on the expression of fnb genes. Mol Microbiol 17:1143–1152
Hanley W, McCarty O, Jadhav S, Tseng Y, Wirtz D, Konstantopoulos K (2003) Single molecule characterization of P-selectin/ligand binding. J Biol Chem 278:10556–10561
Heilmann C, Schweitzer O, Gerke C, Vanittanakom N, Mack D, Götz F (1996) Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol 20:1083–1091
Higgins MJ, Crawford SA, Mulvaney P, Wetherbee R (2002) Characterization of the adhesive mucilages secreted by live diatom cells using atomic force microscopy. Protist 153:25–38
Huff S, Matsuka YV, McGavin MJ, Ingham KC (1994) Interaction of N-Terminal fragments of fibronectin with synthetic and recombinant-D motifs from its binding-protein on Staphylococcus aureus studied using fluorescence anisotropy. J Biol Chem 269:15563–15570
Hutter JL, Bechhoefer J (1993) Calibration of atomic-force microscope tips. Rev Sci Instrum 64:1868–1873
Israelachvili JN (1992) Intermolecular and surface forces. Academic, London
Israelachvili JN, McGuiggan PM (1988) Forces between surfaces in liquids. Science 241:795–800
Jericho SK, Jericho MH, Hubbard T, Kujath M (2004) Micro-electro-mechanical systems microtweezers for the manipulation of bacteria and small particles. Rev Sci Instrum 75:1280–1282
Kellermayer MSZ, Smith SB, Granzier HL, Bustamante C (1997) Folding-unfolding transitions in single titin molecules characterized with laser tweezers. Science 276:1112–1116
Kendall TA, Lower SK (2004) Forces between minerals and biological surfaces in aqueous solution. Adv Agronom 82:1–54
Klein C, Hurlbut CS (1985) Manual of mineralogy. Wiley, New York, NY
Leckband D, Israelachvili JN (2001) Intermolecular forces in biology. Quart Rev Biophys 34:105–267
Lee G, Abdi K, Jiang Y, Michaely P, Bennett V, Marszalek PE (2006) Nanospring behaviour of ankyrin repeats. Nature 440:246–249
Lower SK, Hochella MF, Beveridge T (2001a) Bacterial recognition of mineral surfaces: nanoscale interactions between Shewanella and alpha-FeOOH. Science 292:1360–1363
Lower BH, Shi L, Yongsunthon R, Droubay TC, McCready DE, Lower SK (2007) Specific bonds between an iron oxide surface and outer membrane cytochromes MtrC and OmcA from Shewanella oneidensis MR-1. J Bacteriol 189:4944–4952
Lower SK, Tadanier CJ, Hochella MF (2000) Measuring interfacial and adhesion forces between bacteria and mineral surfaces with biological force microscopy. Geochim Cosmochim Acta 64:3133–3139
Lower SK, Tadanier CJ, Hochella MF (2001b) Dynamics of the mineral-microbe interface: use of biological force microscopy in biogeochemistry and geomicrobiology. Geomicrobiol J 18:63–76
Lower SK, Tadanier CJ, Hochella MF, Berry DF, Potts M (1999) The bacteria-mineral interface: probing nanoscale forces with biological force microscopy. Geol Soc Am Abstr Prog 31(7):A394
Lower BH, Yongsunthon R, Shi L, Wildling L, Gruber HJ, Wigginton NS, Reardon CL, Pinchuk GE, Droubay TC, Boily JF, Lower SK (2009) Antibody recognition force microscopy shows that outer membrane cytochromes OmcA and MtrC are expressed on the exterior surface of Shewanella oneidensis MR-1. Appl Environ Microbiol 75:2931–2935
Lower BH, Yongsunthon R, Vellano FP, Lower SK (2005) Simultaneous force and fluorescence measurements of a protein that forms a bond between a living bacterium and a solid surface. J Bacteriol 187:2127–2137
Mack D, Fischer W, Krokotsch A, Leopold K, Hartmann R, Egge H, Laufs R (1996) The intercellular adhesin involved in biofilm accumulation of Staphylococcus epidermidis is a linear beta-1,6-linked glucosaminoglycan: purification and structural analysis. J Bacteriol 178:175–183
Madigan MT, Martinko JM, Parker J (2003) Brock biology of microorganisms. Prentice Hall, Upper Saddle River, NJ
Mueller H, Butt HJ, Bamberg E (1999) Force measurements on myelin basic protein adsorbed to mica and lipid bilayer surfaces done with the atomic force microscope. Biophys J 76:1072–1079
Müller DJ, Baumeister W, Engel A (1999) Controlled unzipping of a bacterial surface layer with atomic force microscopy. Proc Natl Acad Sci USA 96:13170–13174
Noy A, Vezenov DV, Lieber CM (1997) Chemical force microscopy. Annu Rev Mater Sci 27:381–421
Oberdörfer Y, Fuchs H, Janshoff A (2000) Conformational analysis of native fibronectin by means of force spectroscopy. Langmuir 16:9955–9958
Oberhauser AF, Marszalek PE, Carrion-Vazquez M, Fernandez JM (1999) Single protein misfolding events captured by atomic force microscopy. Nat Struct Biol 6:1025–1028
Parsek MR, Fuqua C (2004) Biofilms 2003: emerging themes and challenges in studies of surface-associated microbial life. J Bacteriol 186:4427–4440
Prince JL, Dickinson RB (2003) Kinetics and forces of adhesion for a pair of capsular/unencapsulated Staphylococcus mutant strains. Langmuir 19:154–159
Razatos A, Ong Y-L, Sharma MM, Georgiou G (1998) Molecular determinants of bacterial adhesion monitored by atomic force microscopy. Proc Natl Acad Sci USA 95:11059–11064
Rief M, Gautel M, Oesterhelt F, Fernandez JM, Gaub HE (1997) Reversible unfolding of individual titin immunoglobulin domains by AFM. Science 276:1109–1112
Schwarz-Linek U, Höök M, Potts JR (2004) The molecular basis of fibronectin-mediated bacterial adherence to host cells. Mol Microbiol 52:631–641
Senden TJ, Ducker WA (1994) Experimental determination of spring constants in atomic-force microscopy. Langmuir 10:1003–1004
Taylor ES, Lower SK (2008) Thickness and surface density of extracellular polymers on Acidithiobacillus ferrooxidans. Appl Environ Microbiol 74:309–311
Tortonese M (1997) Cantilevers and tips for atomic force microscopy. IEEE Eng Med Biol Mag 16:2833
Tskhovrebova L, Trinick J, Sleep JA, Simmons RM (1997) Elasticity and unfolding of single molecules of the giant muscle protein titin. Nature 387:308–312
Watnick P, Kolter R (2000) Biofilm, city of microbes. J Bacteriol 182:2675–2679
Whitman WB, Coleman DC, Wiebe WJ (1998) Prokaryotes: the unseen majority. Proc Natl Acad Sci USA 95:6578–6583
Yongsunthon R, Lower SK (2006) Force measurements between a bacterium and another surface in situ. Adv Appl Microbiol 58:97–124
Ziebuhr W, Heilmann C, Götz F, Meyer P, Wilms K, Straube B, Hacker J (1997) Detection of the intercellular adhesion gene cluster (ica) and phase variation in Staphylococcus epidermidis blood culture strains and mucosal isolates. Infect Immun 65:890–896
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media B.V.
About this chapter
Cite this chapter
Lower, S.K. (2011). Atomic Force Microscopy to Study Intermolecular Forces and Bonds Associated with Bacteria. In: Linke, D., Goldman, A. (eds) Bacterial Adhesion. Advances in Experimental Medicine and Biology, vol 715. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0940-9_18
Download citation
DOI: https://doi.org/10.1007/978-94-007-0940-9_18
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-0939-3
Online ISBN: 978-94-007-0940-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)