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Particle Gun Methodology as a Tool in Metabolic Engineering

  • Chapter
Metabolic Engineering of Plant Secondary Metabolism

Summary

Gene transfer into plants is an essential component of any genetic engineering program. Over the past fifteen years major advances in the genetic engineering of plants focused on major crop species such as cereals, legumes and woody species and model systems such as tobacco, petunia and Arabidopsis. This was a reflection of the focus on fundamental studies in gene expression and regulation using single genes or applied targets using genes of agronomic interest such a herbicide, pest and disease resistance. One of the limitations of available methodology has been the difficulty to introgress multiple genes into plant cells. In addition, transformation of more exotic plant species is not straightforward. With the advent of direct DNA transfer procedures such as particle bombardment, it now becomes possible to embark on the genetic engineering of such plants and also to begin considering introduction of multiple genes into plants, taking advantage of co-transformation experiments. This makes the genetic engineering of multi-step pathways a feasible proposition. In this chapter, we will discuss some of the issues involved in such experiments. We will use tobacco as a model to illustrate advantages of particle bombardment in metabolic pathway engineering and we will also discuss experiments involving additional medicinal plants which are now targets for the genetic engineering of secondary metabolism.

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  1. M. J. Leech
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  2. D. Burtin
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  3. D. Hallard
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  8. D. O’Callaghan
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  9. R. Verpoorte
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Editors and Affiliations

  1. Division of Pharmacognosy, Leiden/Amstredam Center for Drug Research, Einsteinweg 55, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    Robert Verpoorte

  2. Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Düsseldorf, Universitätsstr. 1, Geb. 26.13, 40225, Düsseldorf, Germany

    A. W. Alfermann

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Leech, M.J. et al. (2000). Particle Gun Methodology as a Tool in Metabolic Engineering. In: Verpoorte, R., Alfermann, A.W. (eds) Metabolic Engineering of Plant Secondary Metabolism. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9423-3_4

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  • DOI: https://doi.org/10.1007/978-94-015-9423-3_4

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