Introduction
Materials and methods
Materials and chemicals
Isolation, characterization and culturing of the BC-producing strain
BC film preparation and dry weight measurements
Identification and phylogenetic classification of the BC producing isolate
Analytical techniques
Material characterization
Piezoelectric sensitivity measurements
Genome sequencing, assembly and bioinformatics
Results and discussions
Isolation, characterization and classification of the BC-producing strain
Cellulose production in rich medium
Cellulose production in minimal medium
Bacterial strain | Growth medium | Carbon-containing medium components | Production metricsa | References |
---|---|---|---|---|
Glucose | ||||
K. intermedius FST213-1 | HS | 5 g/L YE, 5 g/L Pep | 2.3 g/L (0.01 g/L/h) | Lin et al. (2016) |
K. rhaeticus PG2 | HS | 5 g/L YE, 5 g/L Pep | ~4 g/L (0.01 g/L/h) | Thorat and Dastager (2018) |
K. xylinus B-12068 | HS modified | 5 g/L YE, 5 g/L Pep, 3% Eth | 7.9 g/L (0.05 g/L/h, Flasks) 17.0 g/L (0.1 g/L/h, Petri dish) 6.8 g/L (0.04 g/L/h, Glass tray) | Volova et al. (2018) |
K. hansenii JR-02 | HS modified | 2.5 g/L YE, 2.5 g/L Pep, 2% Eth | 5.0 g/L (0.03 g/L/h) 8.4b g/L (0.05 g/L/h) | Li et al. (2019) |
K. rhaeticus AF1 | HS modifiedc | 5 g/L YE, 5 g/L Pep, 2% Eth | ~6.7 g/L (0.07 g/L/h) | Machado et al. (2016) |
Komagataeibacter sp. W1 | HS | 5 g/L YE, 5 g/L Pep | 1.2 g/L (4 mg/L/h) | Wang et al. (2018) |
K. rhaeticus P 1463 K. hansenii B22 | HS | 5 g/L YE, 5 g/L Pep | 4.4 g/L (0.04 g/L/h) 3.0 g/L (0.03 g/L/h) | Semjonovs et al. (2017) |
G. xylinusd | HS YPD AB-HS | 5 g/L YE, 5 g/L Pep 5 g/L YE, 5 g/L Pep 5 g/L YE, 5 g/L Pep, 100 mM AA | 1.3 g/L (4 mg/L/h) 0.8 g/L (2 mg/L/h) 3.3 g/L (0.01 g/L/h)i, 7.2 g/L (0.03 g/L/h)ii | Kuo et al. (2016) |
K. rhaeticus ENS9a | MA/9e | – | 2.2 g/L (0.01 g/L/h) | This work |
Pure glycerol | ||||
K. rhaeticus PG2 | HS | 5 g/L YE, 5 g/L Pep | ~6.9 g/L (0.02 g/L/h) ~8.7 g/L (0.02 g/L/h)f | Thorat and Dastager (2018) |
K. xylinus B-12068 | HS modified | 5 g/L YE, 5 g/L Pep, 3% Eth | 23.2 g/L (0.14 g/L/h) | Volova et al. (2018) |
K. hansenii JR-02 | HS modified | 2.5 g/L YE, 2.5 g/L Pep | ~2.4 g/L (0.01 g/L/h) | Li et al. (2019) |
Komagataeibacter sp. W1 | HS | 5 g/L YE, 5 g/L Pep | 1.2 g/L (4 mg/L/h) | Wang et al. (2018) |
CGMCC 17276 | AE | 9 g/L YE, 5 g/L Pep, 30 g/L CSL, 0.3% AA, 1.5% Eth | 4.5 g/L (0.05 g/L/h) | Lu et al. (2020) |
K. rhaeticus ENS9a | MA/9g | – | 2.6 g/L (0.01 g/L/h) | This work |
Crude glycerol as a suitable sustainable carbon source for BC production
Bacterial strain | Growth medium | Carbon-containing medium components | Production metricsa | References |
---|---|---|---|---|
G. sacchari | HS | 5 g/L YE, 5 g/L Pep | 0.1 g/L (0.1 mg/L/h) | Carreira et al. (2011) |
G. intermedius NEDO-01 | HSb | 5 g/L YE, 5 g/L Pep | 1.3 g/L (0.01 g/L/h) 3.4 g/L (0.04 g/L/h)i | Kose et al. (2013) |
K. sucrofermentans DSM 15973 | HS | 5 g/L YE, 5 g/L Pep | 3.2 g/L (0.01 g/L/h) 13 g/L (0.04 g/L/h)c | Tsouko et al. (2015) |
G. xylinus BNKC19 | HSd | 5 g/L YE, 5 g/L Pep | 12.3 g/L (0.07 g/L/h) | Soemphol et al. (2018) |
K. saccharivorans | HS | 5 g/L YE, 5 g/L Pep | 12.6 g/L (0.08 g/L/h) | Gayathri and Srinikethan (2018) |
A. xylinum | HS | 5 g/L YE, 5 g/L Pep | ~1.5 g/L (4 mg/L/h)e | Wu et al. (2019) |
G. xylinus KCCM 41431 | HS | 9 g/L YE, 9 g/L Pep | 7 g/L (0.04 g/L/h) | Ho Jin et al. (2019) |
Komagataeibacter sp. nov. CGMCC 17276 | AEf | 5 g/L YE, 5 g/L Pep 30 g/L CSL, 0.7% AA, 0.3% Eth | 4.5 g/L (0.05 g/L/h)i 6 g/L (0.06 g/L/h) | Lu et al. (2020) |
G. xylinus | HS | 5 g/L YE, 5 g/L Pep | ~1.5 g/L (0.01 g/L/h) 2.9 g/L (0.02 g/L/h)g | Dikshit and Kim (2020) |
K. rhaeticus ENS9a | MA/9h | – | 2.9 g/L (0.01 g/L/h) | This work |