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Erschienen in:

2016 | OriginalPaper | Buchkapitel

Characterization of Nanoparticles Under Physiological Conditions

verfasst von : K. A. Eslahian, T. Lang, C. Bantz, R. Keller, R. Sperling, D. Docter, R. Stauber, M. Maskos

Erschienen in: Measuring Biological Impacts of Nanomaterials

Verlag: Springer International Publishing

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Abstract

In this article, well-established characterization methods for nanoparticles (NPs) are discussed, in particular their application under physiological conditions. The impact of different media, mimicking physiological conditions, on NP stability in terms of physiological ionic strength and formation of the NP–protein corona is described. In order to characterize NPs under physiological conditions, we distinguish between scattering and correlation methods, microscopy-based methods, and methods based on hydrodynamic separation. Features and limitations of relevant characterization methods are reviewed, as well as challenges arising in physiological media from enhanced aggregation tendency and the presence of proteins. We conclude that no available method for NP characterization in physiological media is able to describe the colloidal system completely and satisfactory. On the contrary, combining well-chosen analytical methods by taking benefits and disadvantages into account may provide detailed characterization results.

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Nächstes Kapitel Probing the Cytotoxicity of Nanoparticles: Experimental Pitfalls and Artifacts

Linsinger T, Roebben G, Gilliland D, Calzolai L, Rossi F, Gibson P, Klein C (2012) Requirements on measurements for the implementation of the European Commission definition of the term ‘nanomaterial’. Publications Office of the European Union JRC73260, ISBN: 978-92-79-25602-8

Williams D (2008) On the mechanisms of biocompatibility. Biomaterials 29(20):2941–2953CrossRef

Nel A, Xia T, Madler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311(5761):622–627CrossRef

Warheit D (2008) How meaningful are the results of nanotoxicity studies in the absence of adequate material characterization? Toxicol Sci 101(2):183–185CrossRef

Israelachvili J (2011) Intermolecular and surface forces. Elsevier/Academic, Amsterdam

Landau L, Lifshitz E, Pitaevskij L (1998) Electrodynamics of continuous media. Butterworth-Heinemann, Oxford, 8

Russel W, Saville D, Schowalter W (1989) Colloidal dispersions. Cambridge University Press, CambridgeCrossRef

Maskos M, Stauber R (2011) In: Ducheyne P (ed) Comprehensive biomaterials. Elsevier, Oxford

Kasper J, Hermanns M, Bantz C, Koshkina O, Lang T, Maskos M, Pohl C, Unger R, Kirkpatrick C (2013) Interactions of silica nanoparticles with lung epithelial cells and the association to flotillins. Arch Toxicol 87(6):1053–1065CrossRef

10.

Röcker C, Pötzl M, Zhang F, Parak W, Nienhaus G (2009) A quantitative fluorescence study of protein monolayer formation on colloidal nanoparticles. Nat Nanotechnol 4(9):577–580CrossRef

11.

Kittler S, Greulich C, Gebauer J, Diendorf J, Treuel L, Ruiz L, Gonzalez-Calbet J, Vallet-Regi M, Zellner R, Köller M, Epple M (2010) The influence of proteins on the dispersability and cell-biological activity of silver nanoparticles. J Mater Chem 20(3):512CrossRef

12.

Cho K, Lee Y, Lee C-H, Lee K, Kim Y, Choi H, Ryu P-D, Lee S, Joo S-W (2008) Selective aggregation mechanism of unmodified gold nanoparticles in detection of single nucleotide polymorphism. J Phys Chem C 112(23):8629–8633CrossRef

13.

Jiang J, Oberdörster G, Biswas P (2009) Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies. J Nanopart Res 11(1):77–89CrossRef

14.

Gebauer J, Treuel L (2011) Influence of individual ionic components on the agglomeration kinetics of silver nanoparticles. J Colloid Interface Sci 354(2):546–554CrossRef

15.

Derjaguin B, Storozhilova A, Rabinovich Y (1966) Experimental verification of the theory of thermophoresis of aerosol particles. J Colloid Interface Sci 21(1):35–58CrossRef

16.

Holmberg K (2002) Handbook of applied surface and colloid chemistry. Wiley, Chichester

17.

Gregory J (2009) Monitoring particle aggregation processes. Colloids, polymers and surfactants. Special Issue in honour of Brian Vincent, vol 147–148, pp 109–123

18.

Monopoli M, Åberg C, Salvati A, Dawson K (2012) Biomolecular coronas provide the biological identity of nanosized materials. Nat Nanotechnol 7(12):779–786CrossRef

19.

Oberdörster G (2010) Safety assessment for nanotechnology and nanomedicine: concepts of nanotoxicology. J Intern Med 267(1):89–105CrossRef

20.

Oberdörster G (2012) Nanotoxicology: in vitro-in vivo dosimetry. Environ Health Perspect 120(1):A13CrossRef

21.

Vroman L (1962) Effect of adsorbed proteins on the wettability of hydrophilic and hydrophobic solids. Nature 196(4853):476–477CrossRef

22.

Vroman L, Adams A (1969) Findings with the recording ellipsometer suggesting rapid exchange of specific plasma proteins at liquid/solid interfaces. Surf Sci 16:438–446CrossRef

23.

Lynch I, Dawson K (2008) Protein-nanoparticle interactions. Nano Today 3(1–2):40–47CrossRef

24.

Tenzer S, Docter D, Kuharev J, Musyanovych A, Fetz V, Hecht R, Schlenk F, Fischer D, Kiouptsi K, Reinhardt C, Landfester K, Schild H, Maskos M, Knauer S, Stauber R (2013) Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology. Nat Nanotechnol 8(10):772–781CrossRef

25.

Cedervall T, Lynch I, Lindman S, Berggard T, Thulin E, Nilsson H, Dawson K, Linse S (2007) From the cover: understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles. Proc Natl Acad Sci 104(7):2050–2055CrossRef

26.

Monopoli M, Walczyk D, Campbell A, Elia G, Lynch I, Baldelli Bombelli F, Dawson K (2011) Physical − chemical aspects of protein corona: relevance to in vitro and in vivo biological impacts of nanoparticles. J Am Chem Soc 133(8):2525–2534CrossRef

27.

Lundqvist M, Stigler J, Cedervall T, Berggård T, Flanagan M, Lynch I, Elia G, Dawson K (2011) The evolution of the protein corona around nanoparticles: a test study. ACS Nano 5(9):7503–7509CrossRef

28.

Casals E, Pfaller T, Duschl A, Oostingh G, Puntes V (2010) Time evolution of the nanoparticle protein corona. ACS Nano 4(7):3623–3632CrossRef

29.

Tenzer S, Docter D, Rosfa S, Wlodarski A, Kuharev J, Rekik A, Knauer S, Bantz C, Nawroth T, Bier C, Sirirattanapan J, Mann W, Treuel L, Zellner R, Maskos M, Schild H, Stauber R (2011) Nanoparticle size is a critical physicochemical determinant of the human blood plasma corona: a comprehensive quantitative proteomic analysis. ACS Nano 5(9):7155–7167CrossRef

30.

Lundqvist M, Stigler J, Elia G, Lynch I, Cedervall T, Dawson K (2008) Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts. Proc Natl Acad Sci 105(38):14265–14270CrossRef

31.

Mahmoudi M, Serpooshan V (2011) Large protein absorptions from small changes on the surface of nanoparticles. J Phys Chem C 115(37):18275–18283CrossRef

32.

Gebauer J, Malissek M, Simon S, Knauer S, Maskos M, Stauber R, Peukert W, Treuel L (2012) Impact of the nanoparticle–protein corona on colloidal stability and protein structure. Langmuir 28(25):9673–9679CrossRef

33.

Bharti B, Meissner J, Findenegg G (2011) Aggregation of silica nanoparticles directed by adsorption of lysozyme. Langmuir 27(16):9823–9833CrossRef

34.

Bantz C, Koshkina O, Lang T, Galla H-J, Kirkpatrick C, Stauber R, Maskos M (2014) The surface properties of nanoparticles determine the agglomeration state and the size of the particles under physiological conditions. Beilstein J Nanotechnol 5:1774–1786CrossRef

35.

Calvo P, Remuñán-López C, Vila-Jato J, Alonso M (1997) Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers. J Appl Polym Sci 63(1):125–132CrossRef

36.

Nikolic M, Krack M, Aleksandrovic V, Kornowski A, Förster S, Weller H (2006) Tailor-made ligands for biocompatible nanoparticles. Angew Chem Int Ed 45(39):6577–6580CrossRef

37.

Chen H, Wu X, Duan H, Wang Y, Wang L, Zhang M, Mao H (2009) Biocompatible polysiloxane-containing diblock copolymer PEO- b -PγMPS for coating magnetic nanoparticles. ACS Appl Mater Interfaces 1(10):2134–2140CrossRef

38.

Aggarwal P, Hall J, McLeland C, Dobrovolskaia M, McNeil S (2009) Nanoparticle interaction with plasma proteins as it relates to particle biodistribution, biocompatibility and therapeutic efficacy. Adv Drug Deliv Rev 61(6):428–437CrossRef

39.

Dobrovolskaia M, Germolec D, Weaver J (2009) Evaluation of nanoparticle immunotoxicity. Nat Nanotechnol 4(7):411–414CrossRef

40.

Salvati A, Pitek A, Monopoli M, Prapainop K, Bombelli F, Hristov D, Kelly P, Aberg C, Mahon E, Dawson K (2013) Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface. Nat Nanotechnol 8(2):137–143CrossRef

41.

Schärtl W (2007) Light scattering from polymer solutions and nanoparticle dispersions. Springer, Berlin

42.

Lacerda S, Park J, Meuse C, Pristinski D, Becker M, Karim A, Douglas J (2010) Interaction of gold nanoparticles with common human blood proteins. ACS Nano 4(1):365–379CrossRef

43.

Rausch K, Reuter A, Fischer K, Schmidt M (2010) Evaluation of nanoparticle aggregation in human blood serum. Biomacromolecules 11(11):2836–2839CrossRef

44.

Jones C, Grainger D (2009) In vitro assessments of nanomaterial toxicity. Adv Drug Deliv Rev 61(6):438–456CrossRef

45.

Delgado A, González-Caballero F, Hunter R, Koopal L, Lyklema J (2007) Measurement and interpretation of electrokinetic phenomena. Elkin 06, International Electrokinetics Conference, June 25–29, Nancy, France 309(2):194–224

46.

Hunter R (1993) Introduction to modern colloid science. Oxford University Press, Oxford

47.

Lyklema J (1995) Fundamentals of interface and colloid science, Solid-liquid interfaces. Elsevier, Amsterdam, p s.1

48.

Antonietti M, Vorwerg L (1997) Examination of the atypical electrophoretic mobility behavior of charged colloids in the low salt region using the O’Brian-White theory. Colloid Polym Sci 275(9):883–887CrossRef

49.

O’Brien R, White L (1978) Electrophoretic mobility of a spherical colloidal particle. J Chem Soc Faraday Trans 2 74:1607CrossRef

50.

Mangelsdorf C, White L (1990) Effects of stern-layer conductance on electrokinetic transport properties of colloidal particles. Faraday Trans 86(16):2859CrossRef

51.

van Wagenen R, Andrade J (1980) Flat plate streaming potential investigations: hydrodynamics and electrokinetic equivalency. J Colloid Interface Sci 76(2):305–314CrossRef

52.

Hayes R, Böhmer M, Fokkink L (1999) A study of silica nanoparticle adsorption using optical reflectometry and streaming potential techniques. Langmuir 15(8):2865–2870CrossRef

53.

Guinier A, Fournet G (1955) Small-angle scattering of X-rays. Wiley, New York

54.

Glatter O (1977) A new method for the evaluation of small-angle scattering data. J Appl Crystallogr 10(5):415–421CrossRef

55.

McAlister B, Grady B (2002) The use of Monte-Carlo simulations to calculate small-angle scattering patterns. Macromol Symp 190(1):117–130CrossRef

56.

Blanchet C, Svergun D (2013) Small-angle X-Ray scattering on biological macromolecules and nanocomposites in solution. Annu Rev Phys Chem 64(1):37–54CrossRef

57.

Heunemann P, Prévost S, Grillo I, Marino C, Meyer J, Gradzielski M (2011) Formation and structure of slightly anionically charged nanoemulsions obtained by the phase inversion concentration (PIC) method. Soft Matter 7(12):5697CrossRef

58.

Jensen G, Lund R, Gummel J, Monkenbusch M, Narayanan T, Pedersen J (2013) Direct observation of the formation of surfactant micelles under nonisothermal conditions by synchrotron SAXS. J Am Chem Soc 135(19):7214–7222CrossRef

59.

Milani S, Baldelli Bombelli F, Pitek A, Dawson K, Rädler J (2012) Reversible versus irreversible binding of transferrin to polystyrene nanoparticles: soft and hard corona. ACS Nano 6(3):2532–2541CrossRef

60.

Jiang X, Weise S, Hafner M, Rocker C, Zhang F, Parak W, Nienhaus G (2009) Quantitative analysis of the protein corona on FePt nanoparticles formed by transferrin binding. J R Soc Interface 7(Suppl_1):S5CrossRef

61.

Dertinger T, Pacheco V, von der Hocht I, Hartmann R, Gregor I, Enderlein J (2007) Two-focus fluorescence correlation spectroscopy: a New tool for accurate and absolute diffusion measurements. Chemphyschem 8(3):433–443CrossRef

62.

Maffre P, Nienhaus K, Amin F, Parak W, Nienhaus G (2011) Characterization of protein adsorption onto FePt nanoparticles using dual-focus fluorescence correlation spectroscopy. Beilstein J Nanotechnol 2:374–383CrossRef

63.

Maas H, Gruen A, Papantoniou D (1993) Particle tracking velocimetry in three-dimensional flows. Exp Fluids 15(2):133–146CrossRef

64.

Treuel L, Malissek M, Gebauer J, Zellner R (2010) The influence of surface composition of nanoparticles on their interactions with serum albumin. Chemphyschem 11(14):3093–3099CrossRef

65.

Harris J, Roos C, Djalali R, Rheingans O, Maskos M, Schmidt M (1999) Application of the negative staining technique to both aqueous and organic solvent solutions of polymer particles. Micron 30(4):289–298CrossRef

66.

Feynman R, Leighton R, Sands M, Gottlieb M, Leighton R (2006) The Feynman lectures on physics. Pearson Addison-Wesley, San Francisco

67.

Adrian M, Dubochet J, Lepault J, McDowall A (1984) Cryo-electron microscopy of viruses. Nature 308(5954):32–36CrossRef

68.

Mueller W, Koynov K, Fischer K, Hartmann S, Pierrat S, Basché T, Maskos M (2009) Hydrophobic shell loading of PB- b -PEO vesicles. Macromolecules 42(1):357–361CrossRef

69.

Milne J, Borgnia M, Bartesaghi A, Tran E, Earl L, Schauder D, Lengyel J, Pierson J, Patwardhan A, Subramaniam S (2013) Cryo-electron microscopy–a primer for the non-microscopist. FEBS J 280(1):28–45CrossRef

70.

Hamley I, Castelletto V, Fundin J, Yang Z, Crothers M, Attwood D, Talmon Y (2004) Cryo-TEM imaging of block copolymer micelles containing solubilized liquid crystal. Colloid Polym Sci 282(5):514–517CrossRef

71.

Shvartzman-Cohen R, Levi-Kalisman Y, Nativ-Roth E, Yerushalmi-Rozen R (2004) Generic approach for dispersing single-walled carbon nanotubes: the strength of a weak interaction. Langmuir 20(15):6085–6088CrossRef

72.

Binnig G, Quate C (1986) Atomic force microscope. Phys Rev Lett 56(9):930–933CrossRef

73.

Magonov S, Elings V, Whangbo M-H (1997) Phase imaging and stiffness in tapping-mode atomic force microscopy. Surf Sci 375(2–3):L385CrossRef

74.

Haeberle W, Pantea M, Hoerber J (2006) Nanometer-scale heat-conductivity measurements on biological samples. In: Proceedings of the seventh international conference on scanning probe microscopy, sensors and nanostructures 106(8–9):678–686

75.

Berger R, Butt H-J, Retschke M, Weber S (2009) Electrical modes in scanning probe microscopy. Macromol Rapid Commun 30(14):1167–1178CrossRef

76.

Giessibl F (1995) Atomic resolution of the silicon (111)-(7x7) surface by atomic force microscopy. Science 267(5194):68–71CrossRef

77.

Jarvis S, Sader J, Fukuma T (2008) In: Bhushan B, Fuchs H, Tomitori M (eds) Applied scanning probe methods VIII. Springer, Berlin, Heidelberg

78.

Gurevich L, Fojan P, Saxena R, Petersen S (2006) Mounting proteins on metal nanoparticles: statistical analysis of AFM images. 2006 NSTI Nanotechnology Conference and Trade Show, Boston, May 7–11, 2006. Nano Science and Technology Institute, Boston

79.

MacCuspie R (2011) Colloidal stability of silver nanoparticles in biologically relevant conditions. J Nanopart Res 13(7):2893–2908CrossRef

80.

Butt H-J, Cappella B, Kappl M (2005) Force measurements with the atomic force microscope: technique, interpretation and applications. Surf Sci Rep 59(1–6):1–152CrossRef

81.

Pyrgiotakis G, Blattmann C, Pratsinis S, Demokritou P (2013) Nanoparticle–nanoparticle interactions in biological media by atomic force microscopy. Langmuir 29(36):11385–11395CrossRef

82.

Schaefer J, Schulze C, Marxer E, Schaefer U, Wohlleben W, Bakowsky U, Lehr C-M (2012) Atomic force microscopy and analytical ultracentrifugation for probing nanomaterial protein interactions. ACS Nano 6(6):4603–4614CrossRef

83.

Gildings J (1968) Nonequilibrium theory of field-flow fractionation. J Chem Phys 49(1):81–85CrossRef

84.

Giddings J (1993) Field-flow fractionation: analysis of macromolecular, colloidal, and particulate materials. Science 260(5113):1456–1465CrossRef

85.

Schimpf M, Caldwell K, Giddings J (2000) Field-flow fractionation handbook. Wiley-Interscience, New York, Chichester

86.

Wahlund K, Giddings J (1987) Properties of an asymmetrical flow field-flow fractionation channel having one permeable wall. Anal Chem 59(9):1332–1339CrossRef

87.

Hovingh M, Thompson G, Giddings J (1970) Column parameters in thermal field-flow fractionation. Anal Chem 42(2):195–203CrossRef

88.

Giddings J (1973) The conceptual basis of field-flow fractionation. J Chem Educ 50(10):667CrossRef

89.

Davis J, Giddings J (1986) Feasibility study of dielectrical field-flow fractionation. Sep Sci Technol 21(9):969–989CrossRef

90.

Lang T, Eslahian K, Maskos M (2012) Ion effects in field-flow fractionation of aqueous colloidal polystyrene. Macromol Chem Phys 213(22):2353–2361CrossRef

91.

Andreev V, Stefanovich L (1993) Theory of field-flow fractionation with the reversible adsorption on channel walls. Chromatographia 37(5–6):325–328CrossRef

92.

Gigault J, Le Hécho I, Dubascoux S, Potin-Gautier M, Lespes G (2010) Single walled carbon nanotube length determination by asymmetrical-flow field-flow fractionation hyphenated to multi-angle laser-light scattering. J Chromatogr A 1217(50):7891–7897CrossRef

93.

Hagendorfer H, Kaegi R, Traber J, Mertens S, Scherrers R, Ludwig C, Ulrich A (2011) Application of an asymmetric flow field flow fractionation multi-detector approach for metallic engineered nanoparticle characterization – prospects and limitations demonstrated on Au nanoparticles. Anal Chim Acta 706(2):367–378CrossRef

94.

Jungmann N, Schmidt M, Maskos M (2001) Characterization of polyorganosiloxane nanoparticles in aqueous dispersion by asymmetrical flow field-flow fractionation. Macromolecules 34(23):8347–8353CrossRef

95.

Rambaldi D, Reschiglian P, Zattoni A (2011) Flow field-flow fractionation: recent trends in protein analysis. Anal Bioanal Chem 399(4):1439–1447CrossRef

96.

Arfvidsson C, Wahlund K-G (2003) Time-minimized determination of ribosome and tRNA levels in bacterial cells using flow field–flow fractionation. Anal Biochem 313(1):76–85CrossRef

97.

Wittgren B, Wahlund K-G, Andersson M, Arfvidsson C (2002) Polysaccharide characterization by flow field-flow fractionation-multiangle light scattering: initial studies of modified starches. Int J Polym Anal Char 7(1–2):19–40CrossRef

98.

Li J, Zhong W (2008) A two-dimensional suspension array system by coupling field flow fractionation to flow cytometry. J Chromatogr A 1183(1–2):143–149CrossRef

99.

Ashby J, Schachermeyer S, Pan S, Zhong W (2013) Dissociation-based screening of nanoparticle–protein interaction via flow field-flow fractionation. Anal Chem 85(15):7494–7501CrossRef

100.

Runyon J, Goering A, Yong K-T, Williams S (2013) Preparation of narrow dispersity gold nanorods by asymmetrical flow field-flow fractionation and investigation of surface Plasmon resonance. Anal Chem 85(2):940–948CrossRef

101.

Rolland-Sabaté A, Mendez-Montealvo M, Colonna P, Planchot V (2008) Online determination of structural properties and observation of deviations from power law behavior. Biomacromolecules 9(7):1719–1730CrossRef

102.

Ehrhart J, Mingotaud A-F, Violleau F (2011) Asymmetrical flow field-flow fractionation with multi-angle light scattering and quasi elastic light scattering for characterization of poly(ethyleneglycol-b-ɛ-caprolactone) block copolymer self-assemblies used as drug carriers for photodynamic therapy. J Chromatogr A 1218(27):4249–4256CrossRef

103.

Schmidt B, Loeschner K, Hadrup N, Mortensen A, Sloth J, Bender Koch C, Larsen E (2011) Quantitative characterization of gold nanoparticles by field-flow fractionation coupled online with light scattering detection and inductively coupled plasma mass spectrometry. Anal Chem 83(7):2461–2468CrossRef

104.

Prestel H, Niessner R, Panne U (2006) Increasing the sensitivity of asymmetrical flow field-flow fractionation: slot outlet technique. Anal Chem 78(18):6664–6669CrossRef

105.

Knappe P, Boehmert L, Bienert R, Karmutzki S, Niemann B, Lampen A, Thünemann A (2011) Processing nanoparticles with A4F-SAXS for toxicological studies: iron oxide in cell-based assays. J Chromatogr A 1218(27):4160–4166CrossRef

106.

Maskos M, Schupp W (2003) Circular asymmetrical flow field-flow fractionation for the semipreparative separation of particles. Anal Chem 75(22):6105–6108CrossRef

107.

Grubisic Z, Rempp P, Benoit H (1967) A universal calibration for gel permeation chromatography. J Polym Sci B Polym Lett 5(9):753–759CrossRef

108.

Hagel L, Lundström H, Andersson T, Lindblom H (1989) Properties, in theory and practice, of novel gel filtration media for standard liquid chromatography. J Chromatogr A 476:329–344CrossRef

109.

Siebrands T, Giersig M, Mulvaney P, Fischer C (1993) Steric exclusion chromatography of nanometer-sized gold particles. Langmuir 9(9):2297–2300CrossRef

110.

Al-Somali A, Krueger K, Falkner J, Colvin V (2004) Recycling size exclusion chromatography for the analysis and separation of nanocrystalline gold. Anal Chem 76(19):5903–5910CrossRef

111.

Pinaud F, King D, Moore H-P, Weiss S (2004) Bioactivation and cell targeting of semiconductor CdSe/ZnS nanocrystals with phytochelatin-related peptides. J Am Chem Soc 126(19):6115–6123CrossRef

112.

Sperling R, Liedl T, Duhr S, Kudera S, Zanella M, Lin C-A, Chang W, Braun D, Parak W (2007) Size determination of (Bio)conjugated water-soluble colloidal nanoparticles: a comparison of different techniques. J Phys Chem C 111(31):11552–11559CrossRef

113.

Wei G-T, Liu F-K, Wang C (1999) Shape separation of nanometer gold particles by size-exclusion chromatography. Anal Chem 71(11):2085–2091CrossRef

114.

Hanauer M, Pierrat S, Zins I, Lotz A, Sönnichsen C (2007) Separation of nanoparticles by gel electrophoresis according to size and shape. Nano Lett 7(9):2881–2885CrossRef

115.

Pellegrino T, Sperling R, Alivisatos A, Parak W (2007) Gel electrophoresis of gold-DNA nanoconjugates. J Biomed Biotech 2007

116.

Shapiro A, Viñuela E, Maizel JV Jr (1967) Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun 28(5):815–820CrossRef

117.

Weber K, Osborn M (1969) The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Bio Chem 244(16):4406–4412

118.

Planken K, Cölfen H (2010) Analytical ultracentrifugation of colloids. Nanoscale 2(10):1849CrossRef

119.

Akbulut O, Mace C, Martinez R, Kumar A, Nie Z, Patton M, Whitesides G (2012) Separation of nanoparticles in aqueous multiphase systems through centrifugation. Nano Lett 12(8):4060–4064CrossRef

120.

Schachman H (1959) Ultracentrifugation in biochemistry. Academic, New York

121.

Liu J, Shire S (1999) Analytical ultracentrifugation in the pharmaceutical industry. J Pharm Sci 88(12):1237–1241CrossRef

122.

Scott D, Harding S, Rowe A (2005) Analytical ultracentrifugation. RSC, CambridgeCrossRef

123.

Domingos R, Baalousha M, Ju-Nam Y, Reid M, Tufenkji N, Lead J, Leppard G, Wilkinson K (2009) Characterizing manufactured nanoparticles in the environment: multimethod determination of particle sizes. Environ Sci Technol 43(19):7277–7284CrossRef

Titel: Characterization of Nanoparticles Under Physiological Conditions
verfasst von: K. A. Eslahian
T. Lang
C. Bantz
R. Keller
R. Sperling
D. Docter
R. Stauber
M. Maskos
Verlag: Springer International Publishing
Buch: Measuring Biological Impacts of Nanomaterials
Print ISBN: 978-3-319-24821-9

Electronic ISBN: 978-3-319-24823-3

Copyright-Jahr: 2016
DOI: https://doi.org/10.1007/11663_2014_10

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