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Erschienen in: Colloid and Polymer Science 8/2016

01.08.2016 | Original Contribution

Characterization of the mechanical properties of cross-linked serum albumin microcapsules: effect of size and protein concentration

verfasst von: Jonathan Gubspun, Pierre-Yves Gires, Clément de Loubens, Dominique Barthès-Biesel, Julien Deschamps, Marc Georgelin, Marc Leonetti, Eric Leclerc, Florence Edwards-Lévy, Anne-Virginie Salsac

Erschienen in: Colloid and Polymer Science | Ausgabe 8/2016

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Abstract

A microfluidic technique is used to characterize the mechanical behavior of capsules that are produced in a two-step process: first, an emulsification step to form droplets, followed by a cross-linking step to encapsulate the droplets within a thin membrane composed of cross-linked proteins. The objective is to study the influence of the capsule size and protein concentration on the membrane mechanical properties. The microcapsules are fabricated by cross-linking of human serum albumin (HSA) with concentrations from 15 to 35 % (w/v). A wide range of capsule radii (∼40–450 μm) is obtained by varying the stirring speed in the emulsification step. For each stirring speed, a low threshold value in protein concentration is found, below which no coherent capsules could be produced. The smaller the stirring speed, the lower the concentration can be. Increasing the concentration from the threshold value and considering capsules of a given size, we show that the surface shear modulus of the membrane increases with the concentration following a sigmoidal curve. The increase in mechanical resistance reveals a higher degree of cross-linking in the membrane. Varying the stirring speed, we find that the surface shear modulus strongly increases with the capsule radius: its increase is two orders of magnitude larger than the increase in size for the capsules under consideration. It demonstrates that the cross-linking reaction is a function of the emulsion size distribution and that capsules produced in batch through emulsification processes inherently have a distribution in mechanical resistance.

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Metadaten
Titel
Characterization of the mechanical properties of cross-linked serum albumin microcapsules: effect of size and protein concentration
verfasst von
Jonathan Gubspun
Pierre-Yves Gires
Clément de Loubens
Dominique Barthès-Biesel
Julien Deschamps
Marc Georgelin
Marc Leonetti
Eric Leclerc
Florence Edwards-Lévy
Anne-Virginie Salsac
Publikationsdatum
01.08.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
Colloid and Polymer Science / Ausgabe 8/2016
Print ISSN: 0303-402X
Elektronische ISSN: 1435-1536
DOI
https://doi.org/10.1007/s00396-016-3885-8

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