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2017 | Supplement | Buchkapitel

Computational Immunohistochemistry: Recipes for Standardization of Immunostaining

verfasst von : Nuri Murat Arar, Pushpak Pati, Aditya Kashyap, Anna Fomitcheva Khartchenko, Orcun Goksel, Govind V. Kaigala, Maria Gabrani

Erschienen in: Medical Image Computing and Computer-Assisted Intervention − MICCAI 2017

Verlag: Springer International Publishing

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Abstract

Cancer diagnosis and personalized cancer treatment are heavily based on the visual assessment of immunohistochemically-stained tissue specimens. The precision of this assessment depends critically on the quality of immunostaining, which is governed by a number of parameters used in the staining process. Tuning of the staining-process parameters is mostly based on pathologists’ qualitative assessment, which incurs inter- and intra-observer variability. The lack of standardization in staining across pathology labs leads to poor reproducibility and consequently to uncertainty in diagnosis and treatment selection. In this paper, we propose a methodology to address this issue through a quantitative evaluation of the staining quality by using visual computing and machine learning techniques on immunohistochemically-stained tissue images. This enables a statistical analysis of the sensitivity of the staining quality to the process parameters and thereby provides an optimal operating range for obtaining high-quality immunostains. We evaluate the proposed methodology on HER2-stained breast cancer tissues and demonstrate its use to define guidelines to optimize and standardize immunostaining.

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Literatur
1.
Zurück zum Zitat Vyberg, M., Nielsen, S.: Proficiency testing in immunohistochemistry - experiences from Nordic Immunohistochemical Quality Control (NordiQC). Virchows Arch. 468, 19–29 (2016)CrossRef Vyberg, M., Nielsen, S.: Proficiency testing in immunohistochemistry - experiences from Nordic Immunohistochemical Quality Control (NordiQC). Virchows Arch. 468, 19–29 (2016)CrossRef
2.
Zurück zum Zitat Brügmann, A., Eld, M., Lelkaitis, G., Nielsen, S., Grunkin, M., Hansen, J.D., Foged, N.T., Vyberg, M.: Digital image analysis of membrane connectivity is a robust measure of HER2 immunostains. Breast Cancer Res. Treat. 132, 41–49 (2012)CrossRef Brügmann, A., Eld, M., Lelkaitis, G., Nielsen, S., Grunkin, M., Hansen, J.D., Foged, N.T., Vyberg, M.: Digital image analysis of membrane connectivity is a robust measure of HER2 immunostains. Breast Cancer Res. Treat. 132, 41–49 (2012)CrossRef
3.
Zurück zum Zitat Laurinaviciene, A., Dasevicius, D., Ostapenko, V., Jarmalaite, S., Lazutka, J., Laurinavicius, A.: Membrane connectivity estimated by digital image analysis of HER2 immunohistochemistry is concordant with visual scoring and fluorescence in situ hybridization results: algorithm evaluation on breast cancer tissue microarrays. Diagn. Pathol. 6, 87 (2011)CrossRef Laurinaviciene, A., Dasevicius, D., Ostapenko, V., Jarmalaite, S., Lazutka, J., Laurinavicius, A.: Membrane connectivity estimated by digital image analysis of HER2 immunohistochemistry is concordant with visual scoring and fluorescence in situ hybridization results: algorithm evaluation on breast cancer tissue microarrays. Diagn. Pathol. 6, 87 (2011)CrossRef
4.
Zurück zum Zitat Pinard, R., Tedeschi, G.R., Wang, D., Williams, C.: Methods and system for validating sample images for quantitative immunoassays. US Patent 8160348 (2009) Pinard, R., Tedeschi, G.R., Wang, D., Williams, C.: Methods and system for validating sample images for quantitative immunoassays. US Patent 8160348 (2009)
5.
Zurück zum Zitat Grunkin, M., Hansen, J.D.: Assessment of staining quality. International Patent WO2015135550 (2015) Grunkin, M., Hansen, J.D.: Assessment of staining quality. International Patent WO2015135550 (2015)
6.
Zurück zum Zitat Brügmann, A., Grunkin, M., Nielsen, S., Jensen, V., Heikkila, P., Gaspar, V., Vyberg, M.: Image analysis of breast cancer HER2 protein expression used in assessment of staining quality. Virchows Arch. 465, S20 (2014) Brügmann, A., Grunkin, M., Nielsen, S., Jensen, V., Heikkila, P., Gaspar, V., Vyberg, M.: Image analysis of breast cancer HER2 protein expression used in assessment of staining quality. Virchows Arch. 465, S20 (2014)
7.
Zurück zum Zitat Kaigala, G.V., Lovchik, R.D., Drechsler, U., Delamarche, E.: A vertical microfluidic probe. Langmuir 27, 5686–5693 (2011)CrossRef Kaigala, G.V., Lovchik, R.D., Drechsler, U., Delamarche, E.: A vertical microfluidic probe. Langmuir 27, 5686–5693 (2011)CrossRef
8.
Zurück zum Zitat Hoda, S.A., Brogi, E., Koerner, F.C., Rosen, P.P.: Rosen’s Breast Pathology. Wolters Kluwer, UK (2014) Hoda, S.A., Brogi, E., Koerner, F.C., Rosen, P.P.: Rosen’s Breast Pathology. Wolters Kluwer, UK (2014)
9.
Zurück zum Zitat Seguin, B., Saab, H., Gabrani, M., Estellers, V.: Estimating pattern sensitivity to the printing process for varying dose/focus conditions for RET development in the sub-22nm era. In: Proceedings of SPIE, vol. 9050 (2014) Seguin, B., Saab, H., Gabrani, M., Estellers, V.: Estimating pattern sensitivity to the printing process for varying dose/focus conditions for RET development in the sub-22nm era. In: Proceedings of SPIE, vol. 9050 (2014)
Metadaten
Titel
Computational Immunohistochemistry: Recipes for Standardization of Immunostaining
verfasst von
Nuri Murat Arar
Pushpak Pati
Aditya Kashyap
Anna Fomitcheva Khartchenko
Orcun Goksel
Govind V. Kaigala
Maria Gabrani
Copyright-Jahr
2017
DOI
https://doi.org/10.1007/978-3-319-66185-8_6