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Erschienen in:
2010 | OriginalPaper | Buchkapitel
Design of an Enterobacteriaceae Pan-Genome Microarray Chip
verfasst von : Oksana Lukjancenko, David W. Ussery
Erschienen in: Computational Systems-Biology and Bioinformatics
Verlag: Springer Berlin Heidelberg
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Microarrays are a common method for evaluating genomic content of bacterial species and comparing unsequenced bacterial genomes. This technology allows for quick scans of characteristic genes and chromosomal regions, and to search for indications of horizontal transfer. A high-density microarray chip has been designed, using 116
Enterobacteriaceae
genome sequences, taking into account the enteric pan-genome. Probes for the microarray were checked
in silico
and performance of the chip, based on experimental strains from four different genera, demonstrate a relatively high ability to distinguish those strains on genus, species, and pathotype/serovar levels. Additionally, the microarray performed well when investigating which genes were found in a given strain of interest. The
Enterobacteriaceae
pan-genome microarray, based on 116 genomes, provides a valuable tool for determination of the genetic makeup of unknown strains within this bacterial family and can introduce insights into phylogenetic relationships.