Monoclonal antibodies against a derivative of diphenylurea and the derived Fab fragments were investigated using a BIACORE® system with respect to binding kinetics, thermodynamics and assay characteristics. The chloroacetyl group of the hapten II used for immunization and immobilization was involved in the antibody binding. The binding of Fab to immobilized hapten was enthalpy driven below 37°C. Ethanol led to decreased affinities, but shifted the crossreactivity in favour of hapten II.
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- Detection of Diphenylurea Derivatives with Biospecific Interaction Analysis (BIA): Kinetic Investigations
W. F. M. Stöcklein
F. W. Scheller
- Springer Netherlands
in-adhesives, MKVS, Hellmich GmbH/© Hellmich GmbH, Zühlke/© Zühlke