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01.02.2013 | Research Paper

Effects of TiO₂ nanoparticles on the NO₂ ⁻ levels in cell culture media analysed by Griess colorimetric methods

verfasst von: Traian Popescu, Andreea R. Lupu, Lucian Diamandescu, Doina Tarabasanu-Mihaila, Valentin S. Teodorescu, Valentin Raditoiu, Violeta Purcar, Aurel M. Vlaicu

Erschienen in: Journal of Nanoparticle Research | Ausgabe 2/2013

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Abstract

The Griess assay has been used to determine the possible changes in the measured NO₂ ⁻ concentrations induced by TiO₂ nanoparticles in three types of nitrite-containing samples: aqueous NaNO₂ solutions with known concentrations, and two types of cell culture media—Roswell Park Memorial Institute medium (RPMI-1640) and Dulbecco’s Modified Eagle Medium (DMEM-F12) used either as delivered or enriched in NO₂ ⁻ by NaNO₂ addition. We have used three types of titania with average particle sizes between 10 and 30 nm: Degussa P25 and two other samples (undoped and Fe³⁺-doped anatase TiO₂) synthesised by a hydrothermal route in our laboratory. The structural, morphological, optical and physicochemical characteristics of the used materials have been studied by X-ray diffraction, transmission electron microscopy (EDX), Mössbauer spectroscopy, Brunauer–Emmett–Teller nitrogen adsorption, UV–Vis reflectance spectroscopy, dynamic light scattering and diffuse reflectance infrared Fourier transform spectroscopy. The opacity and sedimentation behaviour of the studied TiO₂ suspensions have been investigated by photometric attenuance measurements at 540 nm. To account for the photocatalytic properties of titania in a biologically relevant context, multiple Griess tests have been performed under controlled exposure to laboratory natural daylight illumination. The results show significant variations of light attenuance (associated with NO₂ ⁻ concentrations in the Griess test) depending on the opacity, sedimentation behaviour, NO₂ ⁻ adsorption and photocatalytic properties of the tested TiO₂ nanomaterials. These findings identify material characteristics recommended to be considered when analysing the results of Griess tests performed in biological studies involving TiO₂ nanoparticles.

Vorheriger Artikel Temperature-dependent magnetic and structural ordering of self-assembled magnetic array of FePt nanoparticles

Nächster Artikel The effect of alkyl chain length on the level of capping of silicon nanoparticles produced by a one-pot synthesis route based on the chemical reduction of micelle

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Titel: Effects of TiO2 nanoparticles on the NO2 − levels in cell culture media analysed by Griess colorimetric methods
verfasst von: Traian Popescu
Andreea R. Lupu
Lucian Diamandescu
Doina Tarabasanu-Mihaila
Valentin S. Teodorescu
Valentin Raditoiu
Violeta Purcar
Aurel M. Vlaicu
Publikationsdatum: 01.02.2013
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 2/2013
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-013-1449-0

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