Electrochemically synthesized new class of molecularly imprinted poly-rhodamine b nanodots for the detection of nutritional anaemia biomarker-bovine haemoglobin in salt-sick cattle

This paper reports a new type of tailor-made recognition element, molecularly imprinted poly-nanodots for nutritional anaemia biomarker bovine haemoglobin. Herein for the first time, rhodamine b dye is used as an electroactive monomer for formation of a new type of tailor-made recognition element viz. imprinted polymeric nanodots exhibits quantum dots properties. The molecularly imprinted polymeric nanodots were directly synthesized on the surface of graphite electrode by electropolymerization. The resultant molecularly imprinted polymeric-nanodots shows specific recognition to the bovine haemoglobin, their recognition selectivity was compared with non-imprinted polymeric nanodots. The best optimized molecularly imprinted poly-nanodots modified graphite electrodes were morphologically and electrochemically characterized via scanning electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry. Good linearity was obtained for bovine haemoglobin in a range of 0.085–115.5 ng mL⁻¹ and the average recoveries at three spiked levels in aqueous and blood samples of cattle were higher than 98% (RSD < 5%). Limit of quantification and Limit of detection was 0.085 and 0.012 ng mL⁻¹, respectively. Cross-reactivity studies with other proteins interferences were tested. A new type of electrochemical sensing platform exhibited superior selectivity and reproducibility facilitating the determination of BHP in real samples of cattle.