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Published in:

2015 | Online First | Chapter

A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells

Authors : Peter F. Renz, Tobias A. Beyer

Published in: Methods in Molecular Biology™

Publisher: Springer New York

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Abstract

Human embryonic stem cells hold great promise for future biomedical applications such as disease modeling and regenerative medicine. However, these cells are notoriously difficult to culture and are refractory to common means of genetic manipulation, thereby limiting their range of applications. In this protocol, we present an easy and robust method of gene repression in human embryonic stem cells using lipofection of small interfering RNA (siRNA).
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Metadata
Title
A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells
Authors
Peter F. Renz
Tobias A. Beyer
Copyright Year
2015
Publisher
Springer New York
DOI
https://doi.org/10.1007/7651_2015_212