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2018 | OriginalPaper | Chapter

A Continuous Flow Microelectrophoretic Module for Protein Separation

Authors : A. Capuano, A. Adami, V. Mulloni, L. Lorenzelli

Published in: Sensors and Microsystems

Publisher: Springer International Publishing

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Abstract

In this paper, we are presenting the development of a microfluidic electrically driven SPLITT (Split flow thin fractionation) device, which can be used for online protein separation. The microfluidic network is realized by means of a three times laminated structure (thermo-compression lamination) of a thin dry film, Ordyl SY 355, which is patterned through a photolithographic technique. The device has been tested with a Bovine Serum Albumin (BSA) solution, through absorbance measurements with a spectrophotometer, with best achieved separation at the outlet of 40%, measured as relative concentration unbalance at output channels.

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Literature
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Metadata
Title
A Continuous Flow Microelectrophoretic Module for Protein Separation
Authors
A. Capuano
A. Adami
V. Mulloni
L. Lorenzelli
Copyright Year
2018
DOI
https://doi.org/10.1007/978-3-319-66802-4_15