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Arabian Journal for Science and Engineering

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31-03-2020 | Research Article-Biological Sciences

Antibacterial Activity of Lactobacillus plantarum CRL 759 Against Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa

Authors: Barbara I. Layus, Carla L. Gerez, Ana V. Rodriguez

Published in: Arabian Journal for Science and Engineering | Issue 6/2020

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Abstract

This study investigates the antibacterial properties of potential probiotic Lactobacillus plantarum CRL 759 against Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) isolated from samples of human diabetic foot, by different methods. The results demonstrated that L. plantarum CRL 759 cell-free supernatant (SLp759) inhibited both P. aeruginosa and MRSA growth. In addition, SLp759 inhibited the adhesion of pathogenic microbes. The characterization of SLp759 showed that after neutralization of acidic SLp759, no activity against pathogens strains was observed. Moreover, treatment with proteolytic enzymes did not modify antibacterial activity, indicating that no bacteriocin was present in the supernatant. In addition, results obtained by HPLC analysis indicated that the inhibitory effect was the result of production of two organic acids: lactic and acetic. From the results reported here, L. plantarum CRL 759 has potential applications as a probiotic by preventing or breaking multidrug-resistant microorganism carriage.

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Brunel, A.S.; Guery, B.: Multidrug resistant (or antimicrobial-resistant) pathogens - alternatives to new antibiotics? Swiss Med. Wkly. 147, w14553 (2017)

FAO/WHO.: Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada (2002).

Marianelli, C.; Cifani, N.; Pasquali, P.: Evaluation of antimicrobial activity of probiotic bacteria against Salmonella enterica subsp. enterica serovar typhimurium 1344 in a common medium under different environmental conditions. Res. Microbiol. 161, 673–680 (2010)CrossRef

Gutiérrez, S.; Martínez-Blanco, H.; Rodríguez-Aparicio, L.; Ferrero, M.: Effect of fermented broth from lactic acid bacteria on pathogenic bacteria proliferation. J. Dairy Sci. 99, 2654–2665 (2016)CrossRef

Arena, M.; Silvain, A.; Normanno, G.; Grieco, F.: Use of Lactobacillus plantarum strains as a bio-control strategy against food-borne pathogenic microorganisms. Front. Microbiol. 7, 464 (2016)CrossRef

Shokri, D.; Khorasgani, M.; Mohkam, M.; Fatemi, S.: The inhibition effect of Lactobacilli against growth and biofilm formation of Pseudomonas aeruginosa. Probiot. Antimicrob. Proteins 10, 34–42 (2018)CrossRef

Khan, A.; Wilson, B.; Gould, I.: Current and future treatment options for community-associated MRSA infection. Expert Opin. Pharmacother. 19, 457–470 (2018)CrossRef

Serra, R.; Grande, R.; Butrico, L.; Rossi, A.: Chronic wound infections: the role of Pseudomonas aeruginosa and Staphylococcus aureus. Expert Rev. Anti. Infect. Ther. 13, 605–613 (2015)CrossRef

Diaz, M.; Griet, M.; De Moreno, A.; Gonzalez, S.: 52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Effects of lactic acid bacteria on inflammatory cytokines production by ARPE-19 cells. Unpublished results (2016).

10.

Bauer, A.W.; Kirby, W.M.; Sherris, J.C.; Turck, M.: Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45, 493–496 (1966)CrossRef

11.

De Man, J.C.; Rogosa, M.; Sharpe, M.E.: A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23, 130–135 (1960)CrossRef

12.

Dec, M.; Puchalski, A.; Urban-Chmiel, R.; Wernicki, A.: Screening of Lactobacillus strains of domestic goose origin against bacterial poultry pathogens for use as probiotics. Poult. Sci. 93, 2464–2472 (2014)CrossRef

13.

Lin, T.-H.; Pan, T.-M.: Characterization of an antimicrobial substance produced by Lactobacillus plantarum NTU 102. J. Microbiol. Immunol. Infect. 52, 409–417 (2017)CrossRef

14.

Dolan, K.E.; Pizano, J.M.; Gossard, C.M.; Williamson, C.B.: Probiotics and disease: a comprehensive summary-part 6. Skin Health. Integr. Med. (Encinitas) 16, 32–41 (2017)

15.

Finley, H.J.; Gasta, M.G.; Dolan, K.E.; Pizano, J.M.: Probiotics and disease: a comprehensive summary-part 8, gastrointestinal and genitourinary disorders. Integr. Med. (Encinitas) 17, 38–48 (2018)

16.

Fuochi, V.; Coniglio, M.A.; Laghi, L.; Rescifina, A.: Metabolic characterization of supernatants produced by Lactobacillus spp. with in vitro anti-Legionella activity. Front. Microbiol. 10, 1403 (2019)CrossRef

17.

Hu, C.-H.; Ren, L.-Q.; Zhou, Y.; Ye, B.-C.: Characterization of antimicrobial activity of three Lactobacillus plantarum strains isolated from Chinese traditional dairy food. Food Sci Nutr. 7, 1997–2005 (2019)CrossRef

18.

Reuben, R.C.; Roy, P.; Sarkar, S.: Characterization and evaluation of lactic acid bacteria from indigenous raw milk for potential probiotic properties. J. Dairy Sci. 103, 1223–1237 (2020)CrossRef

19.

Boyanova, L.; Gergova, G.; Markovska, R.; Yordanov, D.: Bacteriocin-like inhibitory activities of seven Lactobacillus delbrueckii subsp. bulgaricus strains against antibiotic susceptible and resistant Helicobacter pylori strains. Lett. Appl. Microbiol. 65, 469–474 (2017)CrossRef

20.

Leccese Terraf, M.C.; Juarez Tomás, M.S.; Rault, L.; Le Loir, Y.: In vitro effect of vaginal lactobacilli on the growth and adhesion abilities of uropathogenic Escherichia coli. Arch. Microbiol. 199, 767–774 (2017)CrossRef

21.

Onbas, T.; Osmanagaoglu, O.; Kiran, F.: Potential properties of Lactobacillus plantarum F-10 as a bio-control strategy for wound infections. Probiot. Antimicrob. Proteins 11, 1110–1123 (2019)CrossRef

22.

Hopkins, M.J.; Macfarlane, G.T.: Nondigestible oligosaccharides enhance bacterial colonization resistance against Clostridium difficile in vitro. Appl. Environ. Microbiol. 69, 1920–1927 (2003)CrossRef

23.

Sikorska, H.; Smoragiewicz, W.: Role of probiotics in the prevention and treatment of meticillin-resistant Staphylococcus aureus infections. Int. J. Antimicrob. Agents 42, 475–481 (2013)CrossRef

24.

Aksoy, D.Y.; Unal, S.: New antimicrobial agents for the treatment of gram-positive bacterial infections. Clin. Microbiol. Infect. 14, 411–420 (2008)CrossRef

25.

Ruiz, M.E.; Guerrero, I.C.; Tuazon, C.U.: Endocarditis caused by methicillin-resistant Staphylococcus aureus: treatment failure with linezolid. Clin. Infect. Dis. 35, 1018–1020 (2002)CrossRef

26.

Ruiz, L.; Delgado, S.; Ruas-Madiedo, P.; Margolles, A.: Proteinaceous molecules mediating Bifidobacterium-host interactions. Front. Microbiol. 7, 1193 (2016)CrossRef

27.

Ramos, A.N.; Sesto Cabral, M.E.; Arena, M.E.; Arrighi, C.F.: Compounds from Lactobacillus plantarum culture supernatants with potential pro-healing and anti-pathogenic properties in skin chronic wounds. Pharm. Biol. 53, 350–358 (2015)CrossRef

28.

Sesto Cabral, M.E.; Ramos, A.N.; Macedo, A.J.; Trentin, D.S.: Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity. Pharm. Dev. Technol. 20, 1–8 (2014). https://doi.org/10.3109/10837450.2014.938858 CrossRef

29.

Koo, H.; Allan, R.N.; Howlin, R.P.; Stoodley, P.: Targeting microbial biofilms: current and prospective therapeutic strategies. Nat. Rev. Microbiol. 15, 740–755 (2017)CrossRef

30.

Atanasova, J.; Ivanova, I.: Antibacterial peptides from goat and sheep milk proteins. Biotechnol. Biotechnol. Equip. 24, 1799–1803 (2010)CrossRef

31.

Jalilsood, T.; Baradaran, A.; Song, A.A.-L.; Foo, H.L.: Inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming Lactobacillus isolate: a potential host for the expression of heterologous proteins. Microb. Cell Fact. 14, 96 (2015)CrossRef

32.

Chaudhari, S.; Gokhale, D.: Phenyllactic acid: a potential antimicrobial compound in lactic acid bacteria. J. Bacteriol. Mycol. Open Access 2, 1 (2016)

33.

Chatterjee, M.; D’Morris, S.; Paul, V.; Warrier, S.: Mechanistic understanding of phenyllactic acid mediated inhibition of quorum sensing and biofilm development in Pseudomonas aeruginosa. Appl. Microbiol. Biotechnol. 101, 8223–8236 (2017)CrossRef

Title: Antibacterial Activity of Lactobacillus plantarum CRL 759 Against Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa
Authors: Barbara I. Layus
Carla L. Gerez
Ana V. Rodriguez
Publication date: 31-03-2020
Publisher: Springer Berlin Heidelberg
Published in: Arabian Journal for Science and Engineering / Issue 6/2020
Print ISSN: 2193-567X
Electronic ISSN: 2191-4281
DOI: https://doi.org/10.1007/s13369-020-04491-w

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