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Journal of Sol-Gel Science and Technology

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19-11-2021 | Original Paper: Sol–gel and hybrid materials for biological and health (medical) applications

Antibacterial wollastonite supported excellent proliferation and osteogenic differentiation of human bone marrow derived mesenchymal stromal cells

Authors: Senthil Kumar Venkatraman, Rajan Choudhary, Krishnamurithy Genasan, Malliga Raman Murali, Hanumantha Rao Balaji Raghavendran, Tunku Kamarul, Anushree Suresh, Jayanthi Abraham, Seshasailam Venkateswaran, Abel Livingston, Sasikumar Swamiappan

Published in: Journal of Sol-Gel Science and Technology | Issue 3/2021

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Abstract

Biocompatibility and bacterial infections are the primary concerns associated with the current bone graft substitutes. The application of wollastonite-based scaffolds for bone tissue engineering becomes a novel subject of interest. In the present study, a single phasic wollastonite scaffold was synthesised using citric acid-based sol–gel combustion route. Its physicochemical characteristics, antibacterial properties as well as its biocompatibility and osteogenic induction effect on human bone marrow derived stromal cells (hBMSCs) are yet to be explored. The TGA/DTA, XRD and SEM/EDX confirmed the characteristics of wollastonite. The antibacterial test indicated wollastonite inhibition of 47.81% and 45.54% for gram-positive, Staphylococcus aureus and Staphylococcus epidermidis and 47.04% and 46.07% for gram-negative, Escherichia coli and Pseudomonas aeruginosa bacterial strains, respectively. The SEM micrographs demonstrated an excellent attachment of hBMSCs on wollastonite and comparable to commercial hydroxyapatite (cHA) scaffold. The alamar blue cell proliferation assay confirmed 1.7- and 1.8-fold significant increase in hBMSCs seeded on wollastonite and cHA scaffold, respectively, on day 14 as compared with day 1. The immunohistochemistry analysis on Type-I collagen (Col1) and Bone morphogenetic protein-2 (BMP2) expression on day 14 confirmed the osteogenic differentiation of hBMSCs seeded on wollastonite and comparable with cHA scaffold. In conclusion, wollastonite scaffold has a greater potential to substitute bone grafts in orthopaedic applications.

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Title: Antibacterial wollastonite supported excellent proliferation and osteogenic differentiation of human bone marrow derived mesenchymal stromal cells
Authors: Senthil Kumar Venkatraman
Rajan Choudhary
Krishnamurithy Genasan
Malliga Raman Murali
Hanumantha Rao Balaji Raghavendran
Tunku Kamarul
Anushree Suresh
Jayanthi Abraham
Seshasailam Venkateswaran
Abel Livingston
Sasikumar Swamiappan
Publication date: 19-11-2021
Publisher: Springer US
Published in: Journal of Sol-Gel Science and Technology / Issue 3/2021
Print ISSN: 0928-0707
Electronic ISSN: 1573-4846
DOI: https://doi.org/10.1007/s10971-021-05657-0

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