Top

BioControl

Published in:

10-08-2018

Bacillus sp. strain M10 as a potential biocontrol agent protecting chili pepper and tomato fruits from anthracnose disease caused by Colletotrichum capsici

Authors: Prapasri Srikhong, Kongyuth Lertmongkonthum, Rapeewan Sowanpreecha, Panan Rerngsamran

Published in: BioControl | Issue 6/2018

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Bacillus sp. strain M10 was observed to produce an antifungal protein that inhibits the growth of Colletotrichum capsici, which is the causal agent of anthracnose disease of chili pepper and tomato. Ammonium sulfate precipitation, anion exchange chromatography, and sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the protein was approximately 55.4 kDa. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and a subsequent sequence database search indicated the antifungal protein was most similar to the Bacillus amyloliquefaciens vegetative catalase (KatA) protein. Light microscopy observation revealed that the antifungal protein induced abnormal hyphal elongation and conidial swelling and rupture. The protein considerably inhibited anthracnose development and protected the fruits from C. capsici infection. Thus, Bacillus sp. strain M10 and/or its putative catalase may be useful as a post-harvest biocontrol agent that protects chili pepper and tomato fruits from anthracnose disease caused by C. capsici.

previous article Comparative effects of temperature and thermoregulation on candidate strains of entomopathogenic fungi for Moroccan locust Dociostaurus maroccanus control

next article Biological control of rice sheath blight using hyphae-associated bacteria: development of an in planta screening assay to predict biological control agent performance under field conditions

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Alina SO, Constantinscu F, Petruta CC (2015) Biodiversity of Bacillus subtilis group and beneficial traits of Bacillus species useful in plant protection. Rom Biotech Lett 20:10737–10750

Alvindia DG, Acda MA (2015) The antagonistic effect and mechanisms of Bacillus amyloliquefaciens DGA14 against anthracnose in mango cv. ‘Carabao’. Biocontrol Sci Technol 25:560–572CrossRef

Avis TJ (2007) Antifungal compounds that target fungal membranes: applications in plant disease control. Can J Plant Pathol 29:323–329CrossRef

Barefoot SF, Klaenhammer TR (1983) Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol 45:1808–1815PubMedPubMedCentral

Byrne JM, Hausbeck MK, Hammerschmidt R (1997) Conidial germination and appressorium formation of Colletotrichum coccodes on tomato foliage. Plant Dis 81:715–718CrossRefPubMed

Chowdhury SP, Hartmann A, Gao X, Borriss R (2015) Biocontrol mechanism by root-associated Bacillus amyloliquefaciens FZB42—a review. Front Microbiol 6:780. https://doi.org/10.3389/fmicb.2015.00780 CrossRefPubMedPubMedCentral

Diao YZ, Zhang C, Xu JP, Lin D, Liu L, Mtung’e OG, Liu XL (2015) Genetic differentiation and recombination among geographic populations of the fungal pathogen Colletotrichum truncatum from chili peppers in China. Evol Appl 8:108–118CrossRefPubMed

Dieguez-Uribeondo J, Forster H, Soto-Estrada A, Adaskaveg JE (2005) Subcuticular-intracellular hemibiotrophic and intercellular necrotrophic development of Colletotrichum acutatum on almond. Phytopathology 95:751–758CrossRefPubMed

During K, Porsch P, Mahn A, Brinkmann O, Gieffers W (1999) The non-enzymatic microbicidal activity of lysozymes. FEBS Lett 449:93–100CrossRefPubMed

El-Awady M, Moghaieb EEA, Haggag W, Sawsan Youssef S, El-Sharkawy AM (2008) Transgenic canola plants over-expressing bacterial catalase exhibit enhanced resistance to Peronospora parasitica and Erysiphe polygoni. Arab J Biotechnol 11:71–84

Hou X, Boyetchko SM, Brkic M, Olson D, Ross A, Hegedus D (2006) Characterization of the anti-fungal activity of a Bacillus spp. associated with sclerotia from Sclerotinia sclerotiorum. Appl Microbiol Biotechnol 72:644–653CrossRefPubMed

Kim BS, Hwang BK (2007) Microbial fungicides in the control of plant diseases. J Phytopathol 155:641–653CrossRef

Kim PI, Ryu J, Kim YH, Chi YT (2010) Production of biosurfactant lipopeptides iturin A, fengycin and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. J Microbiol Biotechnol 20:138–145PubMed

Kim JD, Jeon BJ, Han JW, Park MY, Kang SA, Kim BS (2016) Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and its efficacy in the control of anthracnose. Pest Manag Sci 72:1529–1536CrossRefPubMed

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefPubMed

Lavermicocca P, Lonigro SL, Evidente A, Andolfi A (1999) Bacteriocin production by Pseudomonas syringae pv. ciccaronei NCPPB2355. Isolation and partial characterization of the antimicrobial compound. J Appl Microbiol 86:257–265CrossRef

Liao CY, Chen MY, Chen YK, Kuo KC, Chung KR, Lee MH (2012) Formation of highly branched hyphae by Colletotrichum acutatum within the fruit cuticles of Capsicum spp. Plant Pathol 61:262–270CrossRef

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

Mataragas M, Metaxopoulos J, Galiotou M, Drosinos EH (2003) Influence of pH and temperature on growth and bacteriocin production by Leuconostoc mesenteroides L124 and Lactobacillus curvatus L442. Meat Sci 64:265–271CrossRefPubMed

McLean KS, Roy KW (1991) Weeds as a source of Colletotrichum capsici causing anthracnose on tomato fruit and cotton seedlings. Can J Plant Pathol 13:131–134CrossRef

Meerak J, Lida H, Watanabe Y, Miyashita M, Sato H, Nakagawa Y, Tahara Y (2007) Phylogeny of gamma-polyglutamic acid-producing Bacillus strains isolated from fermented soybean foods manufactured in Asian countries. J Gen Appl Microbiol 53:315–323CrossRefPubMed

Nagorska K, Bikowski M, Obuchowski M (2007) Multicellular behaviour and production of a wide variety of toxic substances support usage of Bacillus subtilis as a powerful biocontrol agent. Acta Biochim Pol 54:495–508

Ni J, Tokuyama S, Sogabe A, Kawamura Y, Tahara Y (2001) Cloning and high expression of catalase gene from Bacillus sp. TE124. J Biosci Bioeng 91:422–424CrossRefPubMed

Rais A, Jabeen Z, Shair F, Hafeez FY, Hassan MN (2017) Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae. PLoS ONE 12(11):e0187412CrossRefPubMedPubMedCentral

Selitrennikoff CP (2001) Antifungal proteins. Appl Environ Microbiol 67:2883–2894CrossRefPubMedPubMedCentral

Shafi J, Tian H, Ji MS (2017) Bacillus species as versatile weapons for plant pathogens: a review. Biotechnol Biotec Eq 31:446–459CrossRef

Sharma RR, Singh D, Singh R (2009) Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: a review. Biol Control 50:205–221CrossRef

Shuqing L, Nan Z, Zhenhua Z, Jia L, Biao S, Ruifu Z, Qirong S (2013) Antagonist Bacillus subtilis HJ5 controls Verticillium wilt of cotton by root colonization and biofilm formation. Biol Fert Soils 49:295–303CrossRef

Wang LT, Lee FL, Tai CJ, Kasai H (2007) Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. Int J Syst Evol Microbiol 57:1846–1850CrossRef

Wang N, Liu M, Guo L, Yang X, Qiu D (2016) A novel protein elicitor (PeBA1) from Bacillus amyloliquefaciens NC6 induces systemic resistance in tobacco. Int J Biol Sci 12:757–767CrossRefPubMedPubMedCentral

Xiao ZJ, Lu JR, Ma CQ, Xu P (2009) Formation and identification of trimethylimidazole during tetramethylpyrazine production from glucose by Bacillus strains. Biotechnol Lett 31:1421–1425CrossRefPubMed

Yamamoto S, Shiraishi S, Suzuki S (2015) Are cyclic lipopeptides produced by Bacillus amyloliquefaciens S13-3 responsible for the plant defence response in strawberry against Colletotrichum gloeosporioides? Lett Appl Microbiol 60:379–386CrossRefPubMed

Title: Bacillus sp. strain M10 as a potential biocontrol agent protecting chili pepper and tomato fruits from anthracnose disease caused by Colletotrichum capsici
Authors: Prapasri Srikhong
Kongyuth Lertmongkonthum
Rapeewan Sowanpreecha
Panan Rerngsamran
Publication date: 10-08-2018
Publisher: Springer Netherlands
Published in: BioControl / Issue 6/2018
Print ISSN: 1386-6141
Electronic ISSN: 1573-8248
DOI: https://doi.org/10.1007/s10526-018-9902-8

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 6/2018

Factors influencing the dispersal of a native parasitoid, Phasgonophora sulcata, attacking the emerald ash borer: implications for biological control

Predicted ecological niches and environmental resilience of different formulations of the biocontrol yeast Candida sake CPA-1 using the Bioscreen C

Effect of host feeding on life history traits of Tamarixia radiata, parasitoid of the Asian citrus psyllid, Diaphorina citri

Comparative effects of temperature and thermoregulation on candidate strains of entomopathogenic fungi for Moroccan locust Dociostaurus maroccanus control

Characterization of new Bacillus thuringiensis strains from Iran, based on cytocidal and insecticidal activity, proteomic analysis and gene content

Rate of consumption, biological parameters, and population growth capacity of Orius laevigatus fed on Spodoptera exigua