Skip to main content
Top
Published in: Cellulose 1/2021

16-10-2020 | Original Research

Bio-inspired biopolymeric coacervation for entrapment and targeted release of anthocyanin

Authors: Rohan Sarkar, Anirban Dutta, Asit Patra, Supradip Saha

Published in: Cellulose | Issue 1/2021

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

In order to provide stability of anthocyanin, a powerful antioxidant, encapsulant was made by utilizing two biopolymers that can be used further for the targeted release due to its intrinsic structure. Present study was carried out to encapsulate anthocyanin in coacervate employing pectin and chitosan. Entrapment efficiency of anthocyanin was evaluated in coacervates prepared in nine different combinations employing three concentration each of pectin (40, 50 and 60 mg mL−1) and chitosan (5, 6 and 7 mg mL−1) in presence of CaCl2. The developed formulation was characterised by FT-IR, XRD and SEM techniques, which clearly depicts ionic interaction between chitosan and pectin. Release pattern of anthocyanin from the coacervate followed Korsmeyer-Peppas model. The n-values calculated from the curves suggested a quasi-Fickian diffusion pattern pH 1.7 and 4.0. However, at pH 7.0, the behaviour of release was presumed to be an anomalous or case-II transport. In-vitro study revealed that bioaccessibility (%) of coacervated formulation (24.3 ± 0.5) was significantly higher than crude extract (12.2 ± 1.0) as well as purified anthocyanin crystals (18.9 ± 0.7). Better bioaccessibility of coacervated anthocyanin provided important information about its potential use in functional foods.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Appendix
Available only for authorised users
Literature
go back to reference Al-Azi SOSM, Tan YTF, Wong TW (2014) Transforming large molecular weight pectin and chitosan into oral protein drug nanoparticulate carrier. React Funct Polym 84:45–52CrossRef Al-Azi SOSM, Tan YTF, Wong TW (2014) Transforming large molecular weight pectin and chitosan into oral protein drug nanoparticulate carrier. React Funct Polym 84:45–52CrossRef
go back to reference Andishmand H, Tabibiazar M, Mohammadifar MA et al (2017) Pectin-zinc-chitosan-polyethylene glycol colloidal nano-suspension as a food grade carrier for colon targeted delivery of resveratrol. Int J Biol Macromol 97:16–22CrossRef Andishmand H, Tabibiazar M, Mohammadifar MA et al (2017) Pectin-zinc-chitosan-polyethylene glycol colloidal nano-suspension as a food grade carrier for colon targeted delivery of resveratrol. Int J Biol Macromol 97:16–22CrossRef
go back to reference Bernabé P, Peniche C, Argüelles-Monal W (2005) Swelling behavior of chitosan/pectin polyelectrolyte complex membranes. Effect of thermal cross-linking. Polymer Bull 55:367–375CrossRef Bernabé P, Peniche C, Argüelles-Monal W (2005) Swelling behavior of chitosan/pectin polyelectrolyte complex membranes. Effect of thermal cross-linking. Polymer Bull 55:367–375CrossRef
go back to reference Bigucci F, Luppi B, Cerchiara T et al (2008) Chitosan/pectin polyelectrolyte complexes: selection of suitable preparative conditions for colon-specific delivery of vancomycin. Eur J Pharm Sci 35:435–441CrossRef Bigucci F, Luppi B, Cerchiara T et al (2008) Chitosan/pectin polyelectrolyte complexes: selection of suitable preparative conditions for colon-specific delivery of vancomycin. Eur J Pharm Sci 35:435–441CrossRef
go back to reference Bouayed J, Hoffmann L, Bohn T (2011) Total phenolics, flavonoids, anthocyanins and antioxidant activity following simulated gastro-intestinal digestion and dialysis of apple varieties: bioaccessibility and potential uptake. Food Chem 128:14–21CrossRef Bouayed J, Hoffmann L, Bohn T (2011) Total phenolics, flavonoids, anthocyanins and antioxidant activity following simulated gastro-intestinal digestion and dialysis of apple varieties: bioaccessibility and potential uptake. Food Chem 128:14–21CrossRef
go back to reference Boyapally H, Nukala RK, Bhujbal P et al (2010) Controlled release from directly compressible theophylline buccal tablets. Coll Surf B Biointerfaces 77(2):227–233CrossRef Boyapally H, Nukala RK, Bhujbal P et al (2010) Controlled release from directly compressible theophylline buccal tablets. Coll Surf B Biointerfaces 77(2):227–233CrossRef
go back to reference Carrillo C, Buvé C, Panozzo A et al (2017) Role of structural barriers in the in vitro bioaccessibility of anthocyanins in comparison with carotenoids. Food Chem 227:271–279CrossRef Carrillo C, Buvé C, Panozzo A et al (2017) Role of structural barriers in the in vitro bioaccessibility of anthocyanins in comparison with carotenoids. Food Chem 227:271–279CrossRef
go back to reference Comunian TA, Thomazini M, Alves AJG et al (2013) Microencapsulation of ascorbic acid by complex coacervation: protection and controlled release. Food Res Int 52:373–379CrossRef Comunian TA, Thomazini M, Alves AJG et al (2013) Microencapsulation of ascorbic acid by complex coacervation: protection and controlled release. Food Res Int 52:373–379CrossRef
go back to reference Dall'Asta M, Calani L, Tedeschi M et al (2012) Identification of microbial metabolites derived from in vitro fecal fermentation of different polyphenolic food sources. Nutrition 28(2):197–203CrossRef Dall'Asta M, Calani L, Tedeschi M et al (2012) Identification of microbial metabolites derived from in vitro fecal fermentation of different polyphenolic food sources. Nutrition 28(2):197–203CrossRef
go back to reference de Souza JRR, de Carvalho JIX, Trevisan MTS et al (2009) Chitosan-coated pectin beads: characterization and in vitro release of mangiferin. Food Hydrocoll 23:2278–2286CrossRef de Souza JRR, de Carvalho JIX, Trevisan MTS et al (2009) Chitosan-coated pectin beads: characterization and in vitro release of mangiferin. Food Hydrocoll 23:2278–2286CrossRef
go back to reference De Yao K, Liu J, Cheng GX et al (1996) Swelling behavior of pectin/chitosan complex films. J Appl Polym Sci 60:279–283CrossRef De Yao K, Liu J, Cheng GX et al (1996) Swelling behavior of pectin/chitosan complex films. J Appl Polym Sci 60:279–283CrossRef
go back to reference Fernández-Hervása MJ, Fellb JT (1998) Pectin/chitosan mixtures as coatings for colon-specific drug delivery: an in vitro evaluation. Int J Pharm 169:115–119CrossRef Fernández-Hervása MJ, Fellb JT (1998) Pectin/chitosan mixtures as coatings for colon-specific drug delivery: an in vitro evaluation. Int J Pharm 169:115–119CrossRef
go back to reference Ghaffari A, Navaee K, Oskoui M et al ( (2007) Preparation and characterization of free mixed-film of pectin/chitosan/Eudragit®RS intended for sigmoidal drug delivery. Eur J Pharm Biopharm 67:175–186 )CrossRef Ghaffari A, Navaee K, Oskoui M et al ( (2007) Preparation and characterization of free mixed-film of pectin/chitosan/Eudragit®RS intended for sigmoidal drug delivery. Eur J Pharm Biopharm 67:175–186 )CrossRef
go back to reference Gil-Izquierdo A, Zafrilla P, Tomás-Barberán (2002) An in vitro method to simulate phenolic compound release from the food matrix in the gastrointestinal tract. Eur Food Res Tech 214:155–159CrossRef Gil-Izquierdo A, Zafrilla P, Tomás-Barberán (2002) An in vitro method to simulate phenolic compound release from the food matrix in the gastrointestinal tract. Eur Food Res Tech 214:155–159CrossRef
go back to reference Huang GQ, Liu LN, Han XN et al (2017) Intestine-targeted delivery potency of the O-carboxymethyl chitosan–gum Arabic coacervate: Effects of coacervation acidity and possible mechanism. Materials Sci Eng C 79:423–429CrossRef Huang GQ, Liu LN, Han XN et al (2017) Intestine-targeted delivery potency of the O-carboxymethyl chitosan–gum Arabic coacervate: Effects of coacervation acidity and possible mechanism. Materials Sci Eng C 79:423–429CrossRef
go back to reference Kamiloglu S, Capanoglu E, Grootaert C et al (2015) Anthocyanin absorption and metabolism by human intestinal Caco-2 cells—a review. Int J Mol Sci 16:21555–21574CrossRef Kamiloglu S, Capanoglu E, Grootaert C et al (2015) Anthocyanin absorption and metabolism by human intestinal Caco-2 cells—a review. Int J Mol Sci 16:21555–21574CrossRef
go back to reference Kim TH, Park YH, Kim KJ et al (2003) Release of albumin from chitosan-coated pectin beads in vitro. Int J Pharm 250:371–383CrossRef Kim TH, Park YH, Kim KJ et al (2003) Release of albumin from chitosan-coated pectin beads in vitro. Int J Pharm 250:371–383CrossRef
go back to reference Konczak I, Zhang W (2004) Anthocyanins—more than nature’s colours. J Biomed Biotech 239–240 Konczak I, Zhang W (2004) Anthocyanins—more than nature’s colours. J Biomed Biotech 239–240
go back to reference Kurukji D, Norton I, Spyropoulos F (2016) Fabrication of sub-micron protein-chitosan electrostatic complexes for encapsulation and pH-modulated delivery of model hydrophilic active compounds. Food Hydrocoll 53:249–260CrossRef Kurukji D, Norton I, Spyropoulos F (2016) Fabrication of sub-micron protein-chitosan electrostatic complexes for encapsulation and pH-modulated delivery of model hydrophilic active compounds. Food Hydrocoll 53:249–260CrossRef
go back to reference Liang L, Wu X, Zhao T et al (2012) In vitro bioaccessibility and antioxidant activity of anthocyanins from mulberry (Morus atropurpurea Roxb.) following simulated gastro-intestinal digestion. Food Res Int 46:76–82CrossRef Liang L, Wu X, Zhao T et al (2012) In vitro bioaccessibility and antioxidant activity of anthocyanins from mulberry (Morus atropurpurea Roxb.) following simulated gastro-intestinal digestion. Food Res Int 46:76–82CrossRef
go back to reference McDougall GJ, Dobson P, Smith P et al (2005) Assessing potential bioavailability of raspberry anthocyanins using an in vitro digestion system. J Agric Food Chem 53:5896–5904CrossRef McDougall GJ, Dobson P, Smith P et al (2005) Assessing potential bioavailability of raspberry anthocyanins using an in vitro digestion system. J Agric Food Chem 53:5896–5904CrossRef
go back to reference Mülleder U, Murkovic M, Pfannhauser W (2002) Urinary excretion of cyanidin glycosides. J Biochem Biophys Methods 53:61–66CrossRef Mülleder U, Murkovic M, Pfannhauser W (2002) Urinary excretion of cyanidin glycosides. J Biochem Biophys Methods 53:61–66CrossRef
go back to reference Naidu VGM, Madhusudhana K, Sashidhar RB et al (2009) Polyelectrolyte complexes of gum kondagogu and chitosan, as diclofenac carriers. Carbohydr Polym 76(3):464–471CrossRef Naidu VGM, Madhusudhana K, Sashidhar RB et al (2009) Polyelectrolyte complexes of gum kondagogu and chitosan, as diclofenac carriers. Carbohydr Polym 76(3):464–471CrossRef
go back to reference Nori MP, Favaro-Trindade CS, Matias de Alencar S et al (2011) Microencapsulation of propolis extract by complex coacervation. LWT-Food Sci Technol 44:429–435CrossRef Nori MP, Favaro-Trindade CS, Matias de Alencar S et al (2011) Microencapsulation of propolis extract by complex coacervation. LWT-Food Sci Technol 44:429–435CrossRef
go back to reference Oidtmann J, Schantz M, Mäder K et al (2012) Preparation and comparative release characteristics of three anthocyanin encapsulation systems. J Agric Food Chem 60:844–851CrossRef Oidtmann J, Schantz M, Mäder K et al (2012) Preparation and comparative release characteristics of three anthocyanin encapsulation systems. J Agric Food Chem 60:844–851CrossRef
go back to reference Pandey S, Mishra A, Raval P et al (2013) Chitosan-pectin polyelectrolyte complex as a carrier for colon targeted drug delivery. J Young Pharm 5:160–166CrossRef Pandey S, Mishra A, Raval P et al (2013) Chitosan-pectin polyelectrolyte complex as a carrier for colon targeted drug delivery. J Young Pharm 5:160–166CrossRef
go back to reference Saha S, Singh J, Paul A et al (2020) Anthocyanin Profiling Using UV-Vis Spectroscopy and Liquid Chromatography Mass Spectrometry. J AOAC Int 103(1):23–39CrossRef Saha S, Singh J, Paul A et al (2020) Anthocyanin Profiling Using UV-Vis Spectroscopy and Liquid Chromatography Mass Spectrometry. J AOAC Int 103(1):23–39CrossRef
go back to reference Sarkar R, Kundu A, Banerjee K et al (2018) Anthocyanin composition and potential bioactivity of karonda (Carissa carandas) L. fruit: an Indian source of biocolorant. LWT-Food Sci Technol 93:673–678CrossRef Sarkar R, Kundu A, Banerjee K et al (2018) Anthocyanin composition and potential bioactivity of karonda (Carissa carandas) L. fruit: an Indian source of biocolorant. LWT-Food Sci Technol 93:673–678CrossRef
go back to reference Shaddel R, Hesari J, Azadmard-Damirchi S et al (2018) Double emulsion followed by complex coacervation as a promising method for protection of black raspberry anthocyanins. Food Hydrocoll 77:803–816CrossRef Shaddel R, Hesari J, Azadmard-Damirchi S et al (2018) Double emulsion followed by complex coacervation as a promising method for protection of black raspberry anthocyanins. Food Hydrocoll 77:803–816CrossRef
go back to reference Silva DF, Favaro-Trindade CS, Rocha GA et al (2012) Microencapsulation of lycopene by gelatin-pectin complex coacervation. J Food Proc Preserv 36:185–190CrossRef Silva DF, Favaro-Trindade CS, Rocha GA et al (2012) Microencapsulation of lycopene by gelatin-pectin complex coacervation. J Food Proc Preserv 36:185–190CrossRef
go back to reference Talavera S, Felgines C, Texier O et al (2003) Anthocyanins are efficiently absorbed from the stomach in anesthetized rats. J Nutr 133:4178–4182CrossRef Talavera S, Felgines C, Texier O et al (2003) Anthocyanins are efficiently absorbed from the stomach in anesthetized rats. J Nutr 133:4178–4182CrossRef
go back to reference Yang M, Koo SI, Song WO et al (2011) Food matrix affecting anthocyanin bioavailability: review. Curr Med Chem 18:291–300CrossRef Yang M, Koo SI, Song WO et al (2011) Food matrix affecting anthocyanin bioavailability: review. Curr Med Chem 18:291–300CrossRef
go back to reference Yao KD, Tu H, Cheng F et al (1997) pH-sensitivity of the swelling of a chitosan-pectin polyelectrolyte complex. Die Angewandte Makromolekulare Chemie 245:63–72CrossRef Yao KD, Tu H, Cheng F et al (1997) pH-sensitivity of the swelling of a chitosan-pectin polyelectrolyte complex. Die Angewandte Makromolekulare Chemie 245:63–72CrossRef
Metadata
Title
Bio-inspired biopolymeric coacervation for entrapment and targeted release of anthocyanin
Authors
Rohan Sarkar
Anirban Dutta
Asit Patra
Supradip Saha
Publication date
16-10-2020
Publisher
Springer Netherlands
Published in
Cellulose / Issue 1/2021
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-020-03523-w

Other articles of this Issue 1/2021

Cellulose 1/2021 Go to the issue