Biobutanol preparation through sugar-rich biomass by Clostridium saccharoperbutylacetonicum conversion using ZnO nanoparticle catalyst

Biobutanol is a renewable, less polluting, and potentially viable alternative fuel to conservative gasoline. Bacterium activation of Clostridium saccharoperbutylacetonicum is one of the essential steps in butanol fabrication used. Among other various techniques, high-temperature tremor at 100 °C for 2 min was initiated to be the best technique for initiation of Clostridium saccharoperbutylacetonicum bacterium applicable as an inoculum in butanol preparation. Estimation of the efficiency of Clostridium saccharoperbutylacetonicum butanol production from synthetic medium containing 80 g/l of glucose at room temperature showed that Clostridium saccharoperbutylacetonicum gave the maximum butanol concentration (10.59 g/l). Three (3) agronomic raw materials, sorghum juice, sugarcane juice, and sugarcane molasses containing 60 g/l of whole sugar, which is involved in glucose required, were used as butanol manufacture mean by bacterium used. Its P_B values were 1.50, 4.77, and 7.65 g/l, respectively. Furthermore, when sugarcane molasses was mixed with P₂ medium (without glucose), and ZnO nanoparticles were added as a catalyst, the P_B value was enhanced by a factor of 11.58 g/l. The findings revealed that sugarcane molasses has the greatest potential for use as a raw material in the production of biobutanol.