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Energy, Ecology and Environment
Published in: Energy, Ecology and Environment 5/2017

24-07-2017 | Original Article

Biohydrogen production by locally isolated facultative bacterial species using the biomass of Eichhornia crassipes: effect of acid and alkali treatment

Authors: Jerry Mechery, B. Biji, Daniya M. Thomas, V. P. Sylas

Published in: Energy, Ecology and Environment | Issue 5/2017

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Abstract

Hydrogen (H2) produced from biological methods is a potential option to meet the growing clean energy needs. The present study aimed to produce biohydrogen by dark fermentation from nuisance aquatic weed, Eichhornia crassipes, using facultative anaerobic bacteria. A total of 12 bacterial strains were isolated from different wastewater sources and were screened for the potential of H2 production using glucose as carbon source. Ten strains showed the H2-producing potential and were identified up to the generic level by biochemical tests. Two strains with higher H2 production were sequenced using PCR technique and identified as Proteus mirabilis and Pseudomonas aeruginosa and selected for the studies with E. crassipes as the substrate. It was found that P. aeruginosa could produce 19.54 ± 0.03% of H2 from 2% acid (H2SO4) treated substrate which was comparatively higher than that of 4 and 8% treatments. P. mirabilis also yielded better results of 5.42 ± 0.02% H2 f or 2% acid (H2SO4) treated substrate than 4 and 8% treatments. In total, 33.52 ± 0.04% of H2 was produced by P. aeruginosa for the substrate treated with 2% alkali (NaOH). It was noted that with respect to P. mirabilis 4% alkali treated substrate yielded a higher percentage of H2 (20.23 ± 0.03%) compared to the other two concentrations. The results indicate that alkali treated substrate produced comparatively higher amount of H2 than that of acid treated substrates. Regarding efficiency, P. aeruginosa was found to be more competent than P. mirabilis.
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Metadata
Title
Biohydrogen production by locally isolated facultative bacterial species using the biomass of Eichhornia crassipes: effect of acid and alkali treatment
Authors
Jerry Mechery
B. Biji
Daniya M. Thomas
V. P. Sylas
Publication date
24-07-2017
Publisher
Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University
Published in
Energy, Ecology and Environment / Issue 5/2017
Print ISSN: 2363-7692
Electronic ISSN: 2363-8338
DOI
https://doi.org/10.1007/s40974-017-0069-4

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