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2021 | OriginalPaper | Chapter

Cell-Free Based Protein Array Technology

Authors : Ryo Morishita, Hirotaka Takahashi, Tatsuya Sawasaki

Published in: Methods of Mathematical Oncology

Publisher: Springer Singapore

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Abstract

Cell-free protein production technology can easily produce recombinant proteins from cDNA temples, because it is synthesized in a tube without cell culture. We developed a wheat cell-free protein production system from washed wheat embryos. Since our cell-free system is based on eukaryotic translational machinery, it is very suitable for synthesis of eukaryotic proteins such as human protein. Using this system, recently we made a protein array technology consisting of proteins synthesized in 384-well formatted plates, and then constructed human protein array consisting of more than 20,000 recombinant human proteins (20K-HuPA). In addition, combinations with AlphaScreen or magnetic plate technology promotes development of a new high-throughput and high-sensitive approach for identification of protein–protein or protein–antibody interaction. Herein, we demonstrate the results of protein interactomes and antibody validation by using protein array.

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Metadata
Title
Cell-Free Based Protein Array Technology
Authors
Ryo Morishita
Hirotaka Takahashi
Tatsuya Sawasaki
Copyright Year
2021
Publisher
Springer Singapore
DOI
https://doi.org/10.1007/978-981-16-4866-3_18

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