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2020 | OriginalPaper | Chapter

4. Cell-Free Biosensing

Author : Yuan Lu

Published in: Cell-Free Synthetic Biology

Publisher: Springer Singapore

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Abstract

Biosensing has precise sensitivity and high specificity, and the potential use of biosensor ranges from environmental monitoring, food safety, to disease diagnosis [1]. Biosensing is using the biological sensing element to detect some substances, include inorganic molecules, disease biomarkers, and others. A typical biosensor usually includes biological sensing elements and transducers. The transducer can convert the chemical information generated during the biochemical reaction process into corresponding physical signals, such as optical signals, magnetic signals, and electrical signals. In recent years, with the development of synthetic biology, a large number of cell-based biosensors have been developed, and their constructions become more complicated. Cell-based biosensors have a wide range of detection ability. However, there are still some issues that need to be addressed, like transmembrane transport limitations, the need to maintain cell activity, and a long time to assay [2]. Another problem is that cell-based biosensors make use of living, genetically modified microorganisms (GMOs). The application of these GMO biosensors is often not possible due to biosafety concerns, as it must be prevented that GMOs are released into the environment. To address these limitations of cell-based biosensors, cell-free synthesis system as a platform for biosensing has been developed [3].

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Literature
1.
go back to reference A.P. Turner, Biosensors: sense and sensibility. Chem. Soc. Rev. 42(8), 3184–3196 (2013)CrossRef A.P. Turner, Biosensors: sense and sensibility. Chem. Soc. Rev. 42(8), 3184–3196 (2013)CrossRef
2.
go back to reference Q. Liu et al., Cell-based biosensors and their application in biomedicine. Chem. Rev. 114(12), 6423–6461 (2014)CrossRef Q. Liu et al., Cell-based biosensors and their application in biomedicine. Chem. Rev. 114(12), 6423–6461 (2014)CrossRef
3.
go back to reference M.T. Smith et al., The emerging age of cell-free synthetic biology. FEBS Lett. 588(17), 2755–2761 (2014)CrossRef M.T. Smith et al., The emerging age of cell-free synthetic biology. FEBS Lett. 588(17), 2755–2761 (2014)CrossRef
4.
go back to reference T. Pellinen, T. Huovinen, M. Karp, A cell-free biosensor for the detection of transcriptional inducers using firefly luciferase as a reporter. Anal. Biochem. 330(1), 52–57 (2004)CrossRef T. Pellinen, T. Huovinen, M. Karp, A cell-free biosensor for the detection of transcriptional inducers using firefly luciferase as a reporter. Anal. Biochem. 330(1), 52–57 (2004)CrossRef
5.
go back to reference C.E. Chambers et al., Identification of N-acylhomoserine lactones in mucopurulent respiratory secretions from cystic fibrosis patients. FEMS Microbiol. Lett. 244(2), 297–304 (2005)CrossRef C.E. Chambers et al., Identification of N-acylhomoserine lactones in mucopurulent respiratory secretions from cystic fibrosis patients. FEMS Microbiol. Lett. 244(2), 297–304 (2005)CrossRef
6.
go back to reference H.L. Barr et al., Diagnostic and prognostic significance of systemic alkyl quinolones for P. aeruginosa in cystic fibrosis: a longitudinal study; response to comments. J. Cyst. Fibros. 16(6), e21 (2017)CrossRef H.L. Barr et al., Diagnostic and prognostic significance of systemic alkyl quinolones for P. aeruginosa in cystic fibrosis: a longitudinal study; response to comments. J. Cyst. Fibros. 16(6), e21 (2017)CrossRef
7.
go back to reference K.Y. Wen et al., A cell-free biosensor for detecting quorum sensing molecules in P. aeruginosa-infected respiratory samples. ACS Synth. Biol. 6(12), 2293–2301 (2017)CrossRef K.Y. Wen et al., A cell-free biosensor for detecting quorum sensing molecules in P. aeruginosa-infected respiratory samples. ACS Synth. Biol. 6(12), 2293–2301 (2017)CrossRef
8.
go back to reference P.L. Voyvodic et al., Plug-and-Play Metabolic Transducers Expand the Chemical Detection Space of Cell-Free Biosensors (2018) P.L. Voyvodic et al., Plug-and-Play Metabolic Transducers Expand the Chemical Detection Space of Cell-Free Biosensors (2018)
9.
go back to reference D.W. Smith, J. Mackenzie, Zika virus and Guillain-Barre syndrome: another viral cause to add to the list. Lancet 387(10027), 1486–1488 (2016)CrossRef D.W. Smith, J. Mackenzie, Zika virus and Guillain-Barre syndrome: another viral cause to add to the list. Lancet 387(10027), 1486–1488 (2016)CrossRef
10.
go back to reference K. Pardee et al., Paper-based synthetic gene networks. Cell 159(4), 940–954 (2014)CrossRef K. Pardee et al., Paper-based synthetic gene networks. Cell 159(4), 940–954 (2014)CrossRef
11.
go back to reference K. Pardee et al., Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell 165(5), 1255–1266 (2016)CrossRef K. Pardee et al., Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell 165(5), 1255–1266 (2016)CrossRef
12.
go back to reference H. Niederholtmeyer, V. Stepanova, S.J. Maerkl, Implementation of cell-free biological networks at steady state. Proc. Natl. Acad. Sci. USA 110(40), 15985–15990 (2013)CrossRef H. Niederholtmeyer, V. Stepanova, S.J. Maerkl, Implementation of cell-free biological networks at steady state. Proc. Natl. Acad. Sci. USA 110(40), 15985–15990 (2013)CrossRef
13.
go back to reference J.J. Tabor, A. Levskaya, C.A. Voigt, Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405(2), 315–324 (2011)CrossRef J.J. Tabor, A. Levskaya, C.A. Voigt, Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405(2), 315–324 (2011)CrossRef
14.
go back to reference P. Jayaraman et al., Cell-free optogenetic gene expression system. ACS Synth. Biol. 7(4), 986–994 (2018)CrossRef P. Jayaraman et al., Cell-free optogenetic gene expression system. ACS Synth. Biol. 7(4), 986–994 (2018)CrossRef
Metadata
Title
Cell-Free Biosensing
Author
Yuan Lu
Copyright Year
2020
Publisher
Springer Singapore
DOI
https://doi.org/10.1007/978-981-13-1171-0_4