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Cellulose
Published in: Cellulose 4/2018

03-03-2018 | Original Paper

Cellulose hydrogel film for spheroid formation of human adipose-derived stemcells

Authors: Hyeon Joo Kim, Rodrigo Castañeda, Tong Ho Kang, Satoshi Kimura, Masahisa Wada, Ung-Jin Kim

Published in: Cellulose | Issue 4/2018

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Abstracts

Herein, we present a new process for the formation of cell spheroids in three-dimensional culture systems using cellulose hydrogel film. Transparent cellulose hydrogel film were prepared via dissolution-regeneration with a LiOH/urea aqueous solution and methanol. The cell viability of the cellulose hydrogel film was equivalent to that of cell tissue culture plate, revealing no cytotoxicity. Spheroids of human adipose-derived stem cells (hASCs) were successfully formed on cellulose hydrogel film. Spheroid size strongly depended on the cell seeding density and culture time, becoming larger with increases in both factors. Cell differentiation simultaneously progressed favorably with the spheroid formation of hASCs. These results suggested that cellulose hydrogel film could provide a new option with regard to tissue engineering applications.
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Appendix
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Metadata
Title
Cellulose hydrogel film for spheroid formation of human adipose-derived stemcells
Authors
Hyeon Joo Kim
Rodrigo Castañeda
Tong Ho Kang
Satoshi Kimura
Masahisa Wada
Ung-Jin Kim
Publication date
03-03-2018
Publisher
Springer Netherlands
Published in
Cellulose / Issue 4/2018
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-018-1732-4

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