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2015 | OriginalPaper | Chapter

1. Cloning and Bioinformatics Analysis of spsC Gene from Sphingomonas sanxanigenens NX02

Authors : Xiaoyan Li, Haidong Huang, Mingming Zhou, Peng Zhang

Published in: Advances in Applied Biotechnology

Publisher: Springer Berlin Heidelberg

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Abstract

Sphingomonas sanxanigenens strain NX02 synthesizes a novel sphingan Ss, which can be used as drilling mud and thickening agent in the recovery of petroleum by water flooding. In order to research genes involved in the biosyntheses of sphingan Ss, strain NX02 was induced by transposon mini-Tn5 on suicide plasmid pUT, and a mutant strain T163, which cannot produce sphingan Ss, was screened. The spsC gene of NX02 was obtained by the method of Tn5 flanking PCR and LP-RAPD. The predicted amino acid sequence of the spsC protein possessed 493 amino acids and a calculated molecular mass of 53.5 kDa. Bioinformatics analysis revealed that spsC had the highest 60 % amino acid sequence identity with polysaccharide biosynthesis protein of Novosphingobium lindaniclasticum LE124. spsC protein had typical polysaccharide polymerases family transmembrane helices, located between amino acids Y13-V44 and P411-L436. The N-terminal sequence of spsC had high identity to chain length determinant protein of Wzz superfamily.

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Metadata
Title
Cloning and Bioinformatics Analysis of spsC Gene from Sphingomonas sanxanigenens NX02
Authors
Xiaoyan Li
Haidong Huang
Mingming Zhou
Peng Zhang
Copyright Year
2015
Publisher
Springer Berlin Heidelberg
DOI
https://doi.org/10.1007/978-3-662-45657-6_1

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