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Published in: Cellulose 14/2021

03-08-2021 | Original Research

Comparison of different Vetiver grass pretreatment techniques and their impact on immobilized butanol production by Clostridium beijerinckii TISTR 1461

Authors: Fuangfa Srisuk, Piyawat Chinwatpaiboon, Thanaphat Atjayutpokin, Akarin Boonsombuti, Ancharida Savarajara, Apanee Luengnaruemitchai

Published in: Cellulose | Issue 14/2021

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Abstract

Immobilization of microbe cells has been considered as a potential alternative for enhancing biobutanol productivity through acetone-butanol-ethanol (ABE) fermentation. Vetiver grass (VG; Vetiveria zizanoides) pretreated by sodium hydroxide (NaOH) or sulphuric acid was used as carrier for immobilized Clostridium beijerinckii TISTR 1461 in ABE fermentation. The removal of the lignin and hemicellulose fractions of VG during the pretreatment resulted in structural changes, especially an increased crystallinity and surface area. The VG pretreated with 6% (w/v) NaOH at 120 °C for 6 h had the best potential as a carrier in ABE fermentation, giving a butanol and total ABE concentration of 11.6 g/L and 19.8 g/L, respectively, approximately 1.3-fold greater than that of the free-cell system. The VG samples were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, and surface area analyses.

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Appendix
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Metadata
Title
Comparison of different Vetiver grass pretreatment techniques and their impact on immobilized butanol production by Clostridium beijerinckii TISTR 1461
Authors
Fuangfa Srisuk
Piyawat Chinwatpaiboon
Thanaphat Atjayutpokin
Akarin Boonsombuti
Ancharida Savarajara
Apanee Luengnaruemitchai
Publication date
03-08-2021
Publisher
Springer Netherlands
Published in
Cellulose / Issue 14/2021
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-021-04101-4

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