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Journal of Materials Science

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16-10-2017 | Biomaterials

Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization

Authors: Xue Li, Lei Tian, Zafar Ali, Wenyan Wang, Qiuyu Zhang

Published in: Journal of Materials Science | Issue 2/2018

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Abstract

Micron-sized flower-like porous Fe₃O₄ microparticles were fabricated by improved solvothermal method. Then, flexible dendrimer-grafted Fe₃O₄@SiO₂–NH₂/PAMAM microcarriers were obtained by functionalized modification of Michael addition and amidation process. The immobilization of penicillin G acylase on the carrier was realized by cross-linking of glutaraldehyde, and we investigated the optimum immobilization conditions. Under the optimal immobilized conditions, the reaction activity could reach 3098.96 U/g. When the reaction temperature was 47 °C, the immobilized PGA had the highest activity of 394.74 U/g, which indicated that the immobilized PGA had better thermal stability than free PGA. The activity of immobilized enzyme maintained 82.14% after reused for nine times, which indicated that the immobilized enzyme had good stability and good industrial application potential.

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Title: Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization
Authors: Xue Li
Lei Tian
Zafar Ali
Wenyan Wang
Qiuyu Zhang
Publication date: 16-10-2017
Publisher: Springer US
Published in: Journal of Materials Science / Issue 2/2018
Print ISSN: 0022-2461
Electronic ISSN: 1573-4803
DOI: https://doi.org/10.1007/s10853-017-1581-9

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