Skip to main content
Top
Published in: Journal of Nanoparticle Research 12/2015

01-12-2015 | Research Paper

Detection and analysis of human serum albumin nanoparticles within phagocytic cells at the resolution of individual live cell or single 3D multicellular spheroid

Authors: Elena Afrimzon, Naomi Zurgil, Maria Sobolev, Yana Shafran, Klaus Langer, Iavor Zlatev, Robert Wronski, Manfred Windisch, Hagen von Briesen, Reinhold Schmidt, Claus Pietrzik, Mordechai Deutsch

Published in: Journal of Nanoparticle Research | Issue 12/2015

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

Since nanoparticles (NPs) have shown great potential in various biomedical applications, live cell response to NPs should be thoroughly explored prior to their in vivo use. In the current study, live cell array (LCA) methodology and unique cell-based assays were used to study the interaction of magnetite (HSA-Mag NP) loaded human serum albumin NPs with phagocytic cells. The LCA enabled cell culturing during HSA-Mag NP accumulation and monolayer or spheroid formation, concomitantly with on-line monitoring of NP internalization. These platforms were also utilized for imaging intercellular links between living cells preloaded with HSA-Mag NP in 2D and 3D resolution. HSA-Mag NP uptake by cells was quantified by imaging, and analyzed using time-resolved measurements. Image analysis of the individual cells in cell populations showed accumulation of HSA-Mag NP by promonocytes and glial cells in a dose- and time-dependent manner. High variability of NP accumulation in individual cells within cell populations, as well as cell subgroups, was evident in both cell types. Following 24 h interaction, uptake of HSA-Mag NP was about 10 times more efficient in glial cells than in activated promonocytes. The presented assays may facilitate detection and analysis of the amount of NPs within individual cells, as well as the rate of NP accumulation and processing in different subsets of living cells. Such data are crucial for estimating predicted drug dosage delivered by NPs, as well as to study possible mechanisms for NP interference with live cells.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Appendix
Available only for authorised users
Literature
go back to reference Blechinger J, Bauer AT, Torrano AA, Gorzelanny C, Bräuchle C, Schneider SW (2013) Uptake kinetics and nanotoxicity of silica nanoparticles are cell type dependent. Small. doi:10.1002/smll.201301004 Blechinger J, Bauer AT, Torrano AA, Gorzelanny C, Bräuchle C, Schneider SW (2013) Uptake kinetics and nanotoxicity of silica nanoparticles are cell type dependent. Small. doi:10.​1002/​smll.​201301004
go back to reference Deutsch M, Deutsch A, Shirihai O, Hurevich I, Afrimzon E, Shafran Y, Zurgil N (2006) A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells. Lab Chip 6:995–1000CrossRef Deutsch M, Deutsch A, Shirihai O, Hurevich I, Afrimzon E, Shafran Y, Zurgil N (2006) A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells. Lab Chip 6:995–1000CrossRef
go back to reference Eustaquio T, Leary JF (2012) Single-cell nanotoxicity assays of superparamagnetic iron oxide nanoparticles. In: Reineke J (ed) Nanotoxicity: methods and protocols. Methods Mol Biol 926:69–85. Humana Press Eustaquio T, Leary JF (2012) Single-cell nanotoxicity assays of superparamagnetic iron oxide nanoparticles. In: Reineke J (ed) Nanotoxicity: methods and protocols. Methods Mol Biol 926:69–85. Humana Press
go back to reference Gao J, Zhang W, Huang P, Zhang B, Zhang X, Xu B (2008) Intracellular spatial control of fluorescent magnetic nanoparticles. J Am Chem Soc 130:3710–3711CrossRef Gao J, Zhang W, Huang P, Zhang B, Zhang X, Xu B (2008) Intracellular spatial control of fluorescent magnetic nanoparticles. J Am Chem Soc 130:3710–3711CrossRef
go back to reference Horie M, Kato H, Fujita K, Endoh S, Iwahashi H (2012) In vitro evaluation of cellular response induced by manufactured nanoparticles. Chem Res Toxicol 25(3):605–619CrossRef Horie M, Kato H, Fujita K, Endoh S, Iwahashi H (2012) In vitro evaluation of cellular response induced by manufactured nanoparticles. Chem Res Toxicol 25(3):605–619CrossRef
go back to reference Hoysentruyt LC, Akgos Z, Seyfried TN (2011) Hypothesis: are neoplastic macrophages/microglia present in glioblastoma multiforme? ASN Neuro 3(4):e00064. doi:10.1042/AN20110011 Hoysentruyt LC, Akgos Z, Seyfried TN (2011) Hypothesis: are neoplastic macrophages/microglia present in glioblastoma multiforme? ASN Neuro 3(4):e00064. doi:10.​1042/​AN20110011
go back to reference Ibuki Y, Toyooka T (2012) Nanoparticle uptake measured by flow cytometry. In: Reineke J (ed) Nanotoxicity: methods and protocols, Methods Mol Biol 926:157–166. Humana Press, ISBN: 978-1-62703-001-4 (Print) 978-1-62703-002-1 (Online) Ibuki Y, Toyooka T (2012) Nanoparticle uptake measured by flow cytometry. In: Reineke J (ed) Nanotoxicity: methods and protocols, Methods Mol Biol 926:157–166. Humana Press, ISBN: 978-1-62703-001-4 (Print) 978-1-62703-002-1 (Online)
go back to reference Kettiger H, Schipanski A, Wick P, Huwyler J (2013) Engineered nanomaterial uptake and tissue distribution: from cell to organism. Int J Nanomed 8:3255–3269 Kettiger H, Schipanski A, Wick P, Huwyler J (2013) Engineered nanomaterial uptake and tissue distribution: from cell to organism. Int J Nanomed 8:3255–3269
go back to reference Kruttwig K, Brueggemann C, Kaijzel E, Vorhagen S, Hilger T, Löwik C, Hoehn M (2009) Development of a three-dimensional in vitro model for longitudinal observation of cell behavior: monitoring by magnetic resonance imaging and optical imaging. Mol Imaging Biol. doi:10.1007/s11307-009-0289-x Kruttwig K, Brueggemann C, Kaijzel E, Vorhagen S, Hilger T, Löwik C, Hoehn M (2009) Development of a three-dimensional in vitro model for longitudinal observation of cell behavior: monitoring by magnetic resonance imaging and optical imaging. Mol Imaging Biol. doi:10.​1007/​s11307-009-0289-x
go back to reference Langer K, Balthasar S, Vogel V, Dinauer N, von Briesen H, Schubert D (2003) Optimization of the preparation process for human serum albumin (HSA) nanoparticles. Int J Pharm 257:169–180CrossRef Langer K, Balthasar S, Vogel V, Dinauer N, von Briesen H, Schubert D (2003) Optimization of the preparation process for human serum albumin (HSA) nanoparticles. Int J Pharm 257:169–180CrossRef
go back to reference Leßig J, Neu B, Glander HJ, Arnhold J, Reibetanz U (2010) Phagocytotic competence of differentiated U937 cells for colloidal drug delivery systems in immune cells. Inflammation 34(2):99–110. doi:10.1007/s10753-010-9213-4 CrossRef Leßig J, Neu B, Glander HJ, Arnhold J, Reibetanz U (2010) Phagocytotic competence of differentiated U937 cells for colloidal drug delivery systems in immune cells. Inflammation 34(2):99–110. doi:10.​1007/​s10753-010-9213-4 CrossRef
go back to reference Love SA, Maurer-Jones MA, Thompson JW, Lin YS, Haynes CL (2012) Assessing nanoparticle toxicity. Annu Rev Anal Chem (Palo Alto Calif) 5:181–205CrossRef Love SA, Maurer-Jones MA, Thompson JW, Lin YS, Haynes CL (2012) Assessing nanoparticle toxicity. Annu Rev Anal Chem (Palo Alto Calif) 5:181–205CrossRef
go back to reference Lukianova-Hleb EY, Ren X, Constantinou PE, Danysh BP, Shenefelt DL, Carson DD, Farach-Carson MC, Kulchitsky VA, Wu X, Wagner DS, Lapotko DO (2012) Improved cellular specificity of plasmonic nanobubbles versus nanoparticles in heterogeneous cell systems. PLoS One 7(4):e34537CrossRef Lukianova-Hleb EY, Ren X, Constantinou PE, Danysh BP, Shenefelt DL, Carson DD, Farach-Carson MC, Kulchitsky VA, Wu X, Wagner DS, Lapotko DO (2012) Improved cellular specificity of plasmonic nanobubbles versus nanoparticles in heterogeneous cell systems. PLoS One 7(4):e34537CrossRef
go back to reference Luo Y, Wang C, Hossain M, Qiao Y, Ma L, An J, Su M (2012) Three-dimensional microtissue assay for high-throughput cytotoxicity of nanoparticles. Anal Chem 84(15):6731–6738CrossRef Luo Y, Wang C, Hossain M, Qiao Y, Ma L, An J, Su M (2012) Three-dimensional microtissue assay for high-throughput cytotoxicity of nanoparticles. Anal Chem 84(15):6731–6738CrossRef
go back to reference Maldonado CR, Salassa L, Gomez-Blanco N, Mareque-Rivas JC (2013) Nano-functionalization of metal complexes for molecular imaging and anticancer therapy. Coord Chem Rev 257(19):2668–2688CrossRef Maldonado CR, Salassa L, Gomez-Blanco N, Mareque-Rivas JC (2013) Nano-functionalization of metal complexes for molecular imaging and anticancer therapy. Coord Chem Rev 257(19):2668–2688CrossRef
go back to reference Mandarano G, Lodhia J, Eu P, Ferris NJ, Davidson R, Cowell SF (2010) Development and use of iron oxide nanoparticles (Part 2): the application of iron oxide contrast agents in MRI. Biomed Imaging Interv J 6(2):e13. doi:10.2349/biij.6.2.e13 CrossRef Mandarano G, Lodhia J, Eu P, Ferris NJ, Davidson R, Cowell SF (2010) Development and use of iron oxide nanoparticles (Part 2): the application of iron oxide contrast agents in MRI. Biomed Imaging Interv J 6(2):e13. doi:10.​2349/​biij.​6.​2.​e13 CrossRef
go back to reference Markovitz-Bishitz Y, Tauber Y, Afrimzon E, Zurgil N, Sobolev M, Shafran Y, Deutsch A, Howitz S, Deutsch M (2010) A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids. Biomaterials 31(32):8436–8444CrossRef Markovitz-Bishitz Y, Tauber Y, Afrimzon E, Zurgil N, Sobolev M, Shafran Y, Deutsch A, Howitz S, Deutsch M (2010) A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids. Biomaterials 31(32):8436–8444CrossRef
go back to reference Martínez-Vera NP, Schmidt R, Langer K, Zlatev I, Wronski R, Auer E, Havas D, Windisch M, von Briesen H, Wagner S, Stab J, Deutsch M, Pietrzik C, Fazekas F, Ropele S (2014) Tracking of magnetite labeled nanoparticles in the rat brain using MRI. PLoS One 9(3):e92068CrossRef Martínez-Vera NP, Schmidt R, Langer K, Zlatev I, Wronski R, Auer E, Havas D, Windisch M, von Briesen H, Wagner S, Stab J, Deutsch M, Pietrzik C, Fazekas F, Ropele S (2014) Tracking of magnetite labeled nanoparticles in the rat brain using MRI. PLoS One 9(3):e92068CrossRef
go back to reference Martins S, Costa-Lima S, Carneiro T, Cordeiro-da-Silva A, Souto EB, Ferreira DC (2012) Solid lipid nanoparticles as intracellular drug transporters: an investigation of the uptake mechanism and pathway. Int J Pharm 430(1):216–227CrossRef Martins S, Costa-Lima S, Carneiro T, Cordeiro-da-Silva A, Souto EB, Ferreira DC (2012) Solid lipid nanoparticles as intracellular drug transporters: an investigation of the uptake mechanism and pathway. Int J Pharm 430(1):216–227CrossRef
go back to reference Meister S, Zlatev I, Stab J, Docter D, Baches S, Stauber RH, Deutsch M, Schmidt R, Ropele S, Windisch M, Langer K, Wagner S, von Briesen H, Weggen S, Pietrzik CU (2013) Nanoparticulate flurbiprofen reduces amyloid-β42 generation in an in vitro blood–brain barrier model. Alzheimers Res Ther 5(6):51. doi:10.1186/alzrt225 CrossRef Meister S, Zlatev I, Stab J, Docter D, Baches S, Stauber RH, Deutsch M, Schmidt R, Ropele S, Windisch M, Langer K, Wagner S, von Briesen H, Weggen S, Pietrzik CU (2013) Nanoparticulate flurbiprofen reduces amyloid-β42 generation in an in vitro blood–brain barrier model. Alzheimers Res Ther 5(6):51. doi:10.​1186/​alzrt225 CrossRef
go back to reference Sahay G, Alakhova DY, Kabanov AV (2010) Endocytosis of nanomedicines. J Control Release 145(3):182–195CrossRef Sahay G, Alakhova DY, Kabanov AV (2010) Endocytosis of nanomedicines. J Control Release 145(3):182–195CrossRef
go back to reference Sebak S, Mirzaei M, Malhotra M, Kulamarva A, Prakash S (2010) Human serum albumin nanoparticles as an efficient noscapine drug delivery system for potential use in breast cancer: preparation and in vitro analysis. Int J Nanomed 5:525–532 Sebak S, Mirzaei M, Malhotra M, Kulamarva A, Prakash S (2010) Human serum albumin nanoparticles as an efficient noscapine drug delivery system for potential use in breast cancer: preparation and in vitro analysis. Int J Nanomed 5:525–532
go back to reference Singhal N, Handa U, Bansal C, Mohan H (2011) Neutrophil phagocytosis by tumor cells: a cytological study. Diagn Cytopathol 39:553–555CrossRef Singhal N, Handa U, Bansal C, Mohan H (2011) Neutrophil phagocytosis by tumor cells: a cytological study. Diagn Cytopathol 39:553–555CrossRef
go back to reference Sriraman SK, Aryasomayajula B, Torchilin VP (2014) Barriers to drug delivery in solid tumors. Tissue Barriers 2(3):e29528CrossRef Sriraman SK, Aryasomayajula B, Torchilin VP (2014) Barriers to drug delivery in solid tumors. Tissue Barriers 2(3):e29528CrossRef
go back to reference Wagner S, Zensi A, Wien SL, Tschickardt SE, Maier W, Vogel T, Worek F, Pietrzik CU, Kreuter J, von Briesen H (2012) Uptake mechanism of ApoE-modified nanoparticles on brain capillary endothelial cells as a blood-brain barrier model. PLoS One 7(3):e32568CrossRef Wagner S, Zensi A, Wien SL, Tschickardt SE, Maier W, Vogel T, Worek F, Pietrzik CU, Kreuter J, von Briesen H (2012) Uptake mechanism of ApoE-modified nanoparticles on brain capillary endothelial cells as a blood-brain barrier model. PLoS One 7(3):e32568CrossRef
go back to reference Weber C, Kreuter J, Langer K (2000) Desolvation process and surface characteristics of HSA-nanoparticles. Int J Pharm 196(2):197–200CrossRef Weber C, Kreuter J, Langer K (2000) Desolvation process and surface characteristics of HSA-nanoparticles. Int J Pharm 196(2):197–200CrossRef
go back to reference Yang Z, Liu ZW, Allaker RP, Reip P, Oxford J, Ahmad Z, Ren G (2010) A review of nanoparticle functionality and toxicity on the central nervous system. J R Soc Interface 7(Suppl 4):S411–S422CrossRef Yang Z, Liu ZW, Allaker RP, Reip P, Oxford J, Ahmad Z, Ren G (2010) A review of nanoparticle functionality and toxicity on the central nervous system. J R Soc Interface 7(Suppl 4):S411–S422CrossRef
go back to reference Zhu M, Nie G, Meng H, Xia T, Nel A, Zhao Y (2013) Physicochemical properties determine nanomaterial cellular uptake, transport, and fate. Acc Chem Res 46(3):622–631CrossRef Zhu M, Nie G, Meng H, Xia T, Nel A, Zhao Y (2013) Physicochemical properties determine nanomaterial cellular uptake, transport, and fate. Acc Chem Res 46(3):622–631CrossRef
go back to reference Zurgil N, Afrimzon E, Deutsch A, Namer Y, Shafran Y, Sobolev M, Tauber Y, Ravid-Hermesh O, Deutsch M (2010) Polymer live-cell array for real-time kinetic imaging of immune cells. Biomaterials 31(18):5022–5029CrossRef Zurgil N, Afrimzon E, Deutsch A, Namer Y, Shafran Y, Sobolev M, Tauber Y, Ravid-Hermesh O, Deutsch M (2010) Polymer live-cell array for real-time kinetic imaging of immune cells. Biomaterials 31(18):5022–5029CrossRef
Metadata
Title
Detection and analysis of human serum albumin nanoparticles within phagocytic cells at the resolution of individual live cell or single 3D multicellular spheroid
Authors
Elena Afrimzon
Naomi Zurgil
Maria Sobolev
Yana Shafran
Klaus Langer
Iavor Zlatev
Robert Wronski
Manfred Windisch
Hagen von Briesen
Reinhold Schmidt
Claus Pietrzik
Mordechai Deutsch
Publication date
01-12-2015
Publisher
Springer Netherlands
Published in
Journal of Nanoparticle Research / Issue 12/2015
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-015-3306-9

Other articles of this Issue 12/2015

Journal of Nanoparticle Research 12/2015 Go to the issue

Premium Partners