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Ex-Situ Remediation of Heavy Metals Contaminated Soils Using Natural Aluminosilicate Minerals Read chapter Read first chapter

2020 | OriginalPaper | Chapter

Determination of Aflatoxin M1 in Raw Milk by the ELISA Method in the Una-Sana Canton

Authors : Huska Jukić, Samira Dedić, Miloš Rodić, Zlatko Jusufhodžić, Dinko Demirović

Published in: 30th Scientific-Experts Conference of Agriculture and Food Industry

Publisher: Springer International Publishing

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Abstract

The presence of aflatoxin M1 (AFM1) in raw milk should be continuously monitored to protect the population from the risks associated with proven toxicity and carcinogenicity. The ELISA method offers many advantages, including shorter time of analysis, simultaneous analysis of a large number of samples, acceptable as a screening method, limited use of organic solvents, absence of complicated sample preparation steps and simplicity of analytical procedure compared to long-lasting and expensive chromatographic techniques. The test is highly sensitive with a detection limit of 0.01 μg/kg and a quantitative range of 0.01–0.1 μg/kg. In the Una-Sana Canton in 2018, 27 samples of raw cow milk were analysed and all the analyses were carried out twice. The highest concentration of AFM1 was in December and it was 0.032 μg/kg, which is a permissible concentration of AFM1 according to the Regulations on Maximum levels for certain contaminants in food, Official Gazette No. 37/09, which is in compliance with European Union regulations (EU) and the Codex Alimentarius Commission (CAC). In a case of a positive result, the samples are analysed several times and sent to a certified chemical laboratory for the super analysis for the confirmation of the results.

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Metadata
Title
Determination of Aflatoxin M1 in Raw Milk by the ELISA Method in the Una-Sana Canton
Authors
Huska Jukić
Samira Dedić
Miloš Rodić
Zlatko Jusufhodžić
Dinko Demirović
Copyright Year
2020
Book
30th Scientific-Experts Conference of Agriculture and Food Industry

Print ISBN: 978-3-030-40048-4

Electronic ISBN: 978-3-030-40049-1

Copyright Year: 2020

DOI
https://doi.org/10.1007/978-3-030-40049-1_33