Dynamics of MTBE-degrading activity in porous media using a large-scale laboratory experiment

Methyl tert-butyl ether (MTBE) is an oxygenated organic compound that tends to form large groundwater contamination plumes. If bioaugmentation is used as a remediation technique, the question of the mobility of the bioactive zone (BAZ) with time is of interest. The objective of this experiment was to study the spatial redistribution of MTBE-biodegradation activity through time, following the injection of a bacterial culture in a homogeneous porous media, at high pressures and concentrations. The experiment was performed using a large-scale aquifer physical model, which can incorporate physicochemical heterogeneities similar to those found in the field, under controlled laboratory conditions. The experimental tank was filled with 1.0-mm-diameter glass beads to represent a homogeneous high hydraulic conductivity porous medium. During inoculation, the bacterial culture was distributed in a circular pattern. Initially it appeared that the BAZ was located in the upstream portion of the inoculated zone, where oxygen was available in conjunction with the inoculated bacteria and MTBE. With time, the BAZ moved upgradient through the whole tank towards the inlet. This implies the successful movement of bacteria from the inoculation area against advective flow into previously sterile zones of the tank. A mass balance showed that dissolved oxygen concentrations were likely not a limiting factor during the experiments.