The micro-stripe was efficiently transferred by the laser interference-induced forward transfer (LIIFT) technique, composed of Ag nanoparticles (NPs). The effects of the fringe period of the two-beam laser interference on the transferred micro-stripe were discussed. The results showed that the fringe period of the laser interference would affect the particle diameter and spatial distribution of Ag NPs in the micro-stripe transferred. While the fringe period was in the range of 10 to 17 μm, the average diameter of the Ag NPs decreased from 126 to 101 nm with the increase of the period. Moreover, the aggregation of the Ag NPs was improved by increasing the period of the transferred Ag micro-stripe. Finally, the transferred Ag micro-stripe could promote surface-enhanced Raman scattering (SERS). The spectrum intensity of the SERS was significantly enhanced while the transferred Ag micro-stripe was used to the Raman spectrum test of the Rhodamine B (RhB), and the intensity of the Raman spectrum for the RhB was improved with the increase of the Ag NPs micro-stripe period. The results suggested a technique to fabricate the Ag NPs with controllable aggregation efficiently, and further the potential application of the LIIFT technique in the SERS chip of sensitivity detection.