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2015 | OriginalPaper | Chapter

Electron Microscopy Protocols for the Study of Hydrocarbon-Producing and Hydrocarbon-Decomposing Microbes: Classical and Advanced Methods

Authors : Kamna Jhamb, Manfred Auer

Publisher: Springer Berlin Heidelberg

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Abstract

One of the fascinating areas of hydrocarbon microbiology biology is the quest for an ultratstructural understanding of (macro)-molecular mechanisms underlying the degradation, synthesis, and intracellular storage of hydrocarbons, which due to their hydrophobic characteristics continuously threaten the integrity of biological membranes. Here we review classical and novel advanced electron microscopy approaches, including correlative light and electron microscopy that in combination with genetics and biochemical experimentation can be utilized to study such hydrocarbon–cell interactions.
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Metadata
Title
Electron Microscopy Protocols for the Study of Hydrocarbon-Producing and Hydrocarbon-Decomposing Microbes: Classical and Advanced Methods
Authors
Kamna Jhamb
Manfred Auer
Copyright Year
2015
Publisher
Springer Berlin Heidelberg
DOI
https://doi.org/10.1007/8623_2015_96