Top

BioControl

Published in:

01-04-2013

Endophytic Bacillus subtilis WH2 containing Pinellia ternata agglutinin showed insecticidal activity against whitebacked planthopper Sogatella furcifera

Authors: Gaofu Qi, XianFang Zhang, Xiuyun Zhao

Published in: BioControl | Issue 2/2013

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Whitebacked planthopper (WBPH; Sogatella furcifera) has become a major threat to rice plants throughout Asia in recent years. In the study reported here, we attempted to biologically control WBPH by using endophytic bacterium strain WH2, which was isolated from rice seedlings and characterized as Bacillus subtilis based on its morphological, physiological, and biochemical characteristics and 16s rRNA gene sequence. Strain WH2 was genetically engineered to express anti-pest Pinellia ternata agglutinin (PTA). The PTA gene (pta) was cloned into plasmid pP43NMK and transformed to strain WH2 for expression, following which one positive transformant was selected by antibiotic resistance and PCR. This strain was denoted WH2::pta. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot analysis demonstrated that recombinant PTA protein was successfully expressed by recombinant strain WH2::pta. After inoculation, the recombinant WH2::pta was able to colonize rice plants and express secreted PTA protein. The results of the anti-planthopper activity assay showed that WH2::pta significantly decreased the survival and fecundity of WBPH fed on rice seedlings inoculated with WH2::pta (p < 0.001). On day 19 post-inoculation, the number of insects on individual rice plants in the control group was 104.7-fold higher than that on plants inoculated with recombinant WH2::pta and 58.2-fold higher than that on plants inoculated with wild-type WH2. By the end of the study period, all rice seedlings in the control group had died, but those inoculated with recombinant or wild-type WH2 were growing well. These results show that recombinant endophytic B. subtilis WH2 expressing PTA protein can endow its host rice plants with resistance against WBPH. This study presents an approach that may be applied in the future for biologically controlling planthopper using recombinant endophytes.

previous article Mycovirus effect on the endophytic establishment of the entomopathogenic fungus Tolypocladium cylindrosporum in tomato and bean plants

next article Field evaluation of entomopathogenic nematodes for biological control of striped flea beetle, Phyllotreta striolata (Coleoptera: Chrysomelidae)

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Azevedo JL Jr, Maccheroni W, Pereira JO, De Araújo WL (2000) Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electron J Biotechnol 3:40–65CrossRef

Barac T, Taghavi S, Borremans B, Provoost A, Oeyen L, Colpaert JV, Vangronsveld J, van der Lelie D (2004) Engineered endophytic bacteria improve phytoremediation of water solution, volatile, organic pollution. Nat Biotechnol 22:583–588PubMedCrossRef

Bong CFJ, Sikorowski PP (1991) Effects of cytoplasmic polyhedrosis virus and bacterial contamination on growth and development of the corn earworm, Helicoverpa zea (Lepidoptera: Noctuidae). J Invertebr Pathol 57:406–412CrossRef

Cai XQ, Fang H, Hu FP (2003) Colonization trends of Bacillus subtilis BS-2 and BS-1 in capsicum with dual-resistant label. J Fujian Agric For Univ 32:41–45

Chen C, Cheng L, Hou RF (1981) Studies on the intracellular yeast-like symbiote in the brown planthopper, Nilaparvata lugens Stål. J Appl Entomol 92:440–449

Chen J, He Y, Zhang Y, Li N, Chen L, Yu X (2009) Effects of insecticides and fungicides on growth of endosymbiotes isolated from the brown planthopper, Nilaparvata lugens. Plant Prot 35:47–51

Choi YJ, Gringorten JL, Belanger L, Morel L, Bourque D, Masson L, Groleau D, Miguez CB (2008) Production of an insecticidal crystal protein from Bacillus thuringiensis by the methylotroph Methylobacterium extorquens. Appl Environ Microbiol 74:5178–5182PubMedCrossRef

Elbeltagy A, Nishioka K, Suzuki H, Sata T, Sato Y, Morosaki H, Mitsui H, Minamisawa K (2000) Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties. Soil Sci Plant Nutr 46:617–629CrossRef

Fahey JW (1988) Endophytic bacteria for the delivery of agrochemicals to plants. In: Cutler HO (ed) Biologically active natural products. American Chemical Society, Washington D.C., USA, pp 120–128

Fahey JW, Dimock MB, Tomasino SF, Taylor JM, Carlson PS (1991) Genetically engineered endophytes as biocontrol agents: a case study in industry. In: Andrews JH, Hirano SS (eds) Microbial ecology of leaves. Springer, New York, USA, pp 402–411

Fitches E, Woodhouse SD, Edwards JP, Gatehouse JA (2001) In vitro and in vivo binding of snowdrop (Galanthus nivalis agglutinin; GNA) and jackbean (Canavalia ensiformis; Con A) lectins within tomato moth (Lacanobia oleracea) larvae; mechanisms of insecticidal action. J Insect Physiol 47:777–787PubMedCrossRef

Foissac X, Loc NT, Christou P, Gatehouse AMR, Gatehouse JA (2000) Resistance to green leafhopper (Nephotettix virescens) and brown planthopper (Nilaparvata lugens) in transgenic rice expressing snowdrop lectin (Galanthus nivalis agglutinin; GNA). J Insect Physiol 46:573–583PubMedCrossRef

Gatehouse AMR, Gatehouse JA, Bharathi M, Spence J, Powell KS (1998) Immunohistochemical and developmental studies to elucidate the mechanism of action of the snowdrop lectin on the rice brown planthopper, Nilaparvata lugens (Stål). J Insect Physiol 44:529–539PubMedCrossRef

Gurulingappa P, Sword GA, Murdoch G, McGee PA (2010) Colonization of crop plants by fungal entomopathogens and their effects on two insect pests when in planta. Biol Control 55:34–41CrossRef

Harman GE, Howell CR, Viterbo A, Chet I, Lorito M (2004) Trichoderma species: opportunistic, avirulent plant symbionts. Nat Rev Microbiol 2:43–56PubMedCrossRef

Heinrichs EA (1994) Biology and management of rice insects. Wiley Eastern, New Delhi, India

Hirai M, Nakamura K, Imai T, Sato T (1993) cDNAs encoding for storage proteins in the tubers of taro (Colocasia esculenta Schott). Jpn J Genet 68:229–236PubMedCrossRef

Huang DF, Pan YH, Zhang SX, Cao J, Yang X, Zhang J, Yi W (1997) The discovery of insecticidal protein against aphids from Pinellia pedatisecta and P. ternata. Sci Agric Sin 30:94

Itoua-Apoyolo C, Drif L, Vassal JM, Debarjac H, Bossy JP, Leclant F, Frutos R (1995) Isolation of multiple subspecies of Bacillus thuringiensis from a population of the European sunflower moth, Homoeosoma nebulella. Appl Environ Microbiol 61:4343–4347PubMed

Kloepper JW, Schroth MN (1978) Plant growth promoting rhizobacteria on radishes. In: Proc 4th Int Conf Plant Pathogenic Bacteria, vol 2. Station de Pathologie Végétale et Phytobactériologie, INRA, Angers, France, pp 879–882

Lampel JS, Canter GL, Dimock MB, Kelly JL, Anderson JJ, Uratani BB, Foulke JS Jr, Turner JT (1994) Integrative cloning, expression, and stability of the cryIA© gene from Bacillus thuringiensis subsp. kurstaki in a recombinant strain of Clavibacter xyli subsp. cynodontis. Appl Environ Microb 60:501–508

Mano H, Tanaka F, Watanabe A, Kaga H, Okunishi S, Morisaki H (2006) Culturable surface and endophytic Bacterial flora of the maturing seeds of rice plants (Oryza sativa) cultivated in a paddy field. Microbes Environ 21:86–100CrossRef

Mukhopadhyay K, Garrison NK, Hinton DM, Bacon CW, Khush GS, Peck HD, Datta N (1996) Identification and characterization of bacterial endophytes of rice. Mycopathologia 134:151–159PubMedCrossRef

Nagadhara D, Ramesh S, Pasalu IC, Rao YK, Krishnaiah NV, Sarma NP, Bown DP, Gatehouse JA, Reddy VD, Rao KV (2003) Transgenic indica rice resistant to sap-sucking insects. Plant Biotechnol J 1:231–240PubMedCrossRef

Nagadhara D, Ramesh S, Pasalu IC, Rao YK, Sarma NP, Reddy VD, Rao KV (2004) Transgenic rice plants expressing the snowdrop lectin gene (gna) exhibit high-level resistance to the whitebacked planthopper (Sogatella furcifera). Theor Appl Genet 109:1399–1405PubMedCrossRef

Nambiar PTC, Ma SW, Iyer VN (1990) Limiting and insect infestation of nitrogen-fixing root nodules of the Pigeon pea (Cajanus cajan) by engineering the expression of an entomocidal gene in its root nodules. Appl Environ Microbiol 56:2866–2869PubMed

Ownley BH, Gwinn KD, Vega FE (2010) Endophytic fungal entomopathogens with activity against plant pathogens: ecology and evolution. BioControl 55:113–128CrossRef

Pan YH, Zhang SX, Cao JP, Yang XM, Zhang J, Ying WZ, Huang DF (1998) The isolation, purification of Pinellia pedatisecta lectin and its activity on aphid-resistance. Prog Nat Sci 8:502–505

Powell KS, Gatehouse AMR, Hilder VA, Gatehouse JA (1993) Antimetabolic effects of plant lectins and plant and fungal enzymes on the nymphal stages of two important rice pest, Nilaparvata lugens and Nephotettix cinciteps. Entomol Exp Appl 66:119–126CrossRef

Powell KS, Gatehouse AMR, Hilder VA, Gatehouse JA (1995) Antifeedant effects of plant lectins and an enzyme on the adult stage of the rice brown planthopper, Nilaparvata lugens. Entomol Exp Appl 75:51–59CrossRef

Qi G, Mao S, Zhu F, Yu Z, Zhao X (2008) In vitro assessment of plant lectins with anti-pinewood nematode activity. J Invertebr Pathol 98:40–45CrossRef

Qi G, Lan N, Ma X, Yu Z, Zhao X (2011) Controlling Myzus persicae with recombinant endophytic fungi Chaetomium globosum expressing Pinellia ternata agglutinin. J Appl Microbiol 110:1314–1322PubMedCrossRef

Qingwen Z, Ping L, Gang W, Qingnian C (1998) On the biochemical mechanism of induced resistance of cotton to cotton bollworm by cutting of young seedling at plumular axis. Acta Phytophy Sin 25:209–212

Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Pare PW, Kloepper JW (2003) Bacterial volatiles promote growth in Arabidopsis. Proc Natl Acad Sci USA 100:4927–4932PubMedCrossRef

Saravanakumar D, Muthumeena K, Lavanya N, Suresh S, Rajendran L, Raguchander T, Samiyappan R (2007) Pseudomonas-induced defence molecules in rice plants against leaffolder (Cnaphalocrocis medinalis) pest. Pest Manag Sci 63:714–721PubMedCrossRef

Saravanakumar D, Lavanya N, Muthumeena B, Raguchander T, Suresh S, Samiyappan R (2008) Pseudomonas fluorescens enhances resistance and natural enemy population in rice plants against leaffolder pest. J Appl Entomol 132:469–479CrossRef

Schroth MN, Hancock JG (1982) Disease-suppressive soil and root-colonizing bacteria. Science 216:1376–1381PubMedCrossRef

Sikora RA, Pocasangre L, zum Felde A, Niere B, Vu TT, Dababat AA (2008) Mutualistic endophytic fungi and in planta suppressiveness to plant parasitic nematodes. Biol Control 46:15–23CrossRef

Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds) (1986) Bergey’s manual of systematic bacteriology, vol 2. Williams & Wilkins, Baltimore, USA

Spizizen J (1958) Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc Natl Acad Sci USA 44:1072–1078PubMedCrossRef

Sumarah MW, Puniani E, Søensen D, Blackwell BA, Miller JD (2010) Secondary metabolites from anti-insect extracts of endophytic fungi isolated from Picea rubens. Phytochemistry 71:760–765PubMedCrossRef

Sun XF, Tang KX, Wan BL, Qi HX, Lu XG (2001) Transgenic rice homozygous lines expressing GNA showed enhanced resistance to rice brown planthopper (in Chinese). Chin Sci Bull 46:1108–1113

Tomasino SF, Leister RT, Dimock MB, Beach RM, Kelly JL (1995) Field performance of Clavibacter xyli subsp. cynodontis expressing the insecticidal protein gene cryIA © of Bacillus thuringiensis against European corn borer in field corn. Biol Control 5:442–448CrossRef

Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354PubMedCrossRef

van Damme EJ, Goossens K, Smeets K, van Leuven F, Verhaet P, Peumans WJ (1995) The major tuber storage protein of Araceae species is a lectin: characterization and molecular cloning of the lectin from Arum maculatum. Plant Physiol 107:1147–1158PubMedCrossRef

Vessey KJ (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255:571–586CrossRef

Vijayasamundeeswari A, Ladhalakshmi D, Sankaralingam A, Samiyappan R (2009) Plant growth promoting rhizobacteria of cotton affecting the developmental stages of Helicoverpa armigera. J Plant Protect Res 49:239–243

Wang PZ, Doi H (1984) Overlapping promoters transcribed by Bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases. J Biol Chem 259:8619–8625PubMed

Widmer F, Seidler RJ, Gillevet PM, Watrud LS, Giovanni GDD (1998) A highly selective PCR protocol for detecting 16S rRNA genes of the genus Pseudomonas (Sensu Stricto) in environmental samples. Appl Environ Microbiol 64:2545–2553PubMed

Wu CF, Khush GS (1985) A new dominant gene for resistance to whitebacked planthopper in rice. Crop Sci 25:505–509CrossRef

Wu XC, Lee W, Tran L, Wong SL (1991) Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases. J Bacteriol 173:4952–4958PubMed

Yao JH, Sun XF, Tang KX (2001) Molecular cloning of lectin gene from Pinellia ternata. J Fudan Univ 40:461–464

Yao JH, Zhao XY, Liao ZH, Lin J, Chen ZH, Chen F, Song J, Sun XF, Tang KX (2003a) Cloning and molecular characterization of a novel lectin gene from Pinellia ternata. Cell Res 13:301–308PubMedCrossRef

Yao JH, Pang YZ, Qi HX, Wan BL, Zhao XY, Kong WW, Sun XF, Tang KX (2003b) Transgenic tobacco expressing Pinellia ternata agglutinin confers enhanced resistance to aphids. Transgenic Res 12:715–722PubMedCrossRef

Zehnder G, Kloepper J, Yao C, Wei G (1997) Induction of systemic resistance in cucumber against cucumber beetles (Coleoptera: Chrysomelidae) by plant growth-promoting rhizobacteria. J Econ Entomol 90:391–396

Zhang HY, Wu XJ, Tang KX, Wang XD, Sun XF, Zhou KD (2003) A primary study of transferring the Pinellia ternata agglutinin (pta) gene into rice and expression (in Chinese). Acta Genet Sin 30:1013–1019

Title: Endophytic Bacillus subtilis WH2 containing Pinellia ternata agglutinin showed insecticidal activity against whitebacked planthopper Sogatella furcifera
Authors: Gaofu Qi
XianFang Zhang
Xiuyun Zhao
Publication date: 01-04-2013
Publisher: Springer Netherlands
Published in: BioControl / Issue 2/2013
Print ISSN: 1386-6141
Electronic ISSN: 1573-8248
DOI: https://doi.org/10.1007/s10526-012-9485-8

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 2/2013

Mycovirus effect on the endophytic establishment of the entomopathogenic fungus Tolypocladium cylindrosporum in tomato and bean plants

Granulovirus formulations efficiently protect stored and field potatoes from Phthorimaea operculella and Tecia solanivora in Costa Rica

Low temperature storage effects on two olive fruit fly parasitoids

Combination of the biocontrol yeast Cryptococcus laurentii with UV-C treatment for control of postharvest diseases of tomato fruit

Starvation and herbivore-induced plant volatiles affect the color preferences of parasitic wasps

Field evaluation of entomopathogenic nematodes for biological control of striped flea beetle, Phyllotreta striolata (Coleoptera: Chrysomelidae)