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Published in: Cellulose 7/2019

27-03-2019 | Original Research

Enzymatic production of cello-oligomers with endoglucanases

Authors: Stefanie Kluge, Benjamin Bonhage, Jörn Viell, Mari Granström, Alois Kindler, Antje C. Spiess

Published in: Cellulose | Issue 7/2019

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Abstract

Cello-oligomers have a wide application range and are gained from three different cello-oligomer production paths, which show different drawbacks, namely acid or alkaline hydrolysis of cellulose, chemical or enzymatic synthesis, and enzymatic hydrolysis of cellulose using cellulase mixtures. Therefore, this study aims at a novel route towards cello-oligomers using purified endoglucanases. From the renewable raw material cellulose, pretreated by an ionic liquid, cello-oligomers with a weight average degree of polymerization (DPW) of 65 were directly obtained by enzymatic hydrolysis. During hydrolysis, between 14.1 and 24.5 mass percent of monomeric and dimeric sugars were formed as byproduct. A second ionic liquid pretreatment of the cellulose, remaining after the first hydrolysis, and a second enzymatic hydrolysis resulted in cello-oligomers with a DP of 35. XRD deconvolution and Raman analysis confirmed that crystallinity remained unchanged during enzymatic cellulose hydrolysis.

Graphical Abstract

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Appendix
Available only for authorised users
Literature
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go back to reference Caulfield DF, Steffes RA (1969) Water-induced recrystallization of cellulose. Tappi 52:1361–1368 Caulfield DF, Steffes RA (1969) Water-induced recrystallization of cellulose. Tappi 52:1361–1368
go back to reference Granström M, Kindler A, Spiess A, et al (2016) Endoglucanase-induced production of cellulose oligomers. US 20160369314 A1 Granström M, Kindler A, Spiess A, et al (2016) Endoglucanase-induced production of cellulose oligomers. US 20160369314 A1
go back to reference Yamasaki N, Ibuki I, Yaginuma Y, Tamura Y (2006) Cellooligosaccharide-containing composition. US 8349365 B2 Yamasaki N, Ibuki I, Yaginuma Y, Tamura Y (2006) Cellooligosaccharide-containing composition. US 8349365 B2
Metadata
Title
Enzymatic production of cello-oligomers with endoglucanases
Authors
Stefanie Kluge
Benjamin Bonhage
Jörn Viell
Mari Granström
Alois Kindler
Antje C. Spiess
Publication date
27-03-2019
Publisher
Springer Netherlands
Published in
Cellulose / Issue 7/2019
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-019-02390-4

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